Fungal community analysis in seawater of the Mariana Trench as estimated by Illumina HiSeq

Wang, Zhipeng; Liu, Zeng-Zhi; Wang, Yilin; Bi, Wanghua; Liu, Lu; Wang, Haiying; Zheng, Yuan; Zhang, Lin-Lin; Hu, Shu-Gang; Xu, Shanshan; Zhang, Peng

doi:10.1039/c8ra10142f

Cited by 21 publications

(15 citation statements)

References 37 publications

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“…These results are consistent with those obtained, at the phylum level, in deep-sea sediments from places such as the Western and Central Pacific, the Mediterranean Sea or the São Paulo Plateau, which show Ascomycota as the most abundant group, together with the presence of Basidiomycota and Chytridiomycota in lower proportions ( Li et al, 2016 , 2019 ; Xu et al, 2016 , 2019 ; Zhang et al, 2016 ; Nagano et al, 2017 ; Barone et al, 2018 ; Wang et al, 2019 ). However, this is the first work that shows, to our knowledge, the fungal class Saccharomycetes as the most abundant, and also highly correlated with Chytridiomycetes, in deep-sea sediments.…”

Section: Resultssupporting

confidence: 92%

“…We also report another group of co-occurring taxa that includes Cladosporium (Dothideomycetes), Aspergillus and Exophiala (Eurotiomycetes), and Armillaria (Agaricomycetes). The co-occurrence and high abundance of Cladosporium and Aspergillus is relatively common in deep-sea sediments (Li et al, 2019;Wang et al, 2019;Xu et al, 2019). However, with the available information it is difficult to determine whether the positive co-occurrence can be coincidental or can indicate a true positive interaction.…”

Section: Resultsmentioning

confidence: 99%

“…In general, Ascomycota and Basidiomycota are the most abundant groups in deep-sea ecosystems, representing between 70–80% and 10–20% of the sequences, respectively. Some of the most abundant filamentous fungal genera include Penicillium , Aspergillus , Cladosporium , and Fusarium , while some of the most abundant yeasts include Rhodotorula , Cryptococcus , Candida , Rhodosporidium , and Metschnikowia ( Li et al, 2016 , 2019 ; Xu et al, 2016 , 2019 ; Zhang et al, 2016 ; Nagano et al, 2017 ; Barone et al, 2018 ; Wang et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Fungal Communities in Sediments Along a Depth Gradient in the Eastern Tropical Pacific

et al. 2020

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Deep waters represent the largest biome on Earth and the largest ecosystem of Costa Rica. Fungi play a fundamental role in global biogeochemical cycling in marine sediments, yet, they remain little explored. We studied fungal diversity and community composition in several marine sediments from 16 locations sampled along a bathymetric gradient (from a depth of 380 to 3,474 m) in two transects of about 1,500 km length in the Eastern Tropical Pacific (ETP) of Costa Rica. Sequence analysis of the V7-V8 region of the 18S rRNA gene obtained from sediment cores revealed the presence of 787 fungal amplicon sequence variants (ASVs). On average, we detected a richness of 75 fungal ASVs per sample. Ascomycota represented the most abundant phylum with Saccharomycetes constituting the dominant class. Three ASVs accounted for ca. 63% of all fungal sequences: the yeast Metschnikowia (49.4%), Rhizophydium (6.9%), and Cladosporium (6.7%). We distinguished a cluster composed mainly by yeasts, and a second cluster by filamentous fungi, but we were unable to detect a strong effect of depth and the overlying water temperature, salinity, dissolved oxygen (DO), and pH on the composition of fungal communities. We highlight the need to understand further the ecological role of fungi in deep-sea ecosystems.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fungal Communities in Sediments Along a Depth Gradient in the Eastern Tropical Pacific

et al. 2020

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“…Fungi perform vital functions as decomposers, driving nutrient cycles in detritus environments, and as parasites and symbionts in the ecosystems ( Webster and Weber, 2007 ). Similar to previous studies ( Xu et al, 2018 ; Wang et al, 2019 ), the relative abundance of fungi (mainly branched with Ascomycete) was highest in the bathypelagic zone ( Figure 2 ). In addition, a fungi-like group labyrinthulomycetes was also enriched in the bathypelagic zone ( Figure 2 ).…”

Section: Discussionsupporting

confidence: 89%

Shift and Metabolic Potentials of Microbial Eukaryotic Communities Across the Full Depths of the Mariana Trench

et al. 2021

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Microbial eukaryotes are widespread and play important roles in marine ecosystems. However, their ecological characteristics in the deep sea (>1,000 m), especially hadal trenches, were largely unknown. Here, we investigated the diversity and metabolic potentials of microbial eukaryotes along the whole water column of the Mariana Trench by metagenomics. Our results showed clear depth-related distribution of microbial eukaryotic community and associated metabolic potentials. Surface seawater was dominated by phototrophic/mixotrophic groups (e.g., Dinoflagellata) and genes involved in biosynthesis (photosynthesis and fatty acid biosynthesis), while deep (bathypelagic and/or hadal) seawaters were enriched with heterotrophic groups (e.g., Bicoecea) and genes related to digestion (lysosomal enzymes and V-type ATPase) and carbohydrate metabolism. Co-occurrence analysis revealed high intra-domain connectivity, indicating that microbial eukaryotic composition was more influenced by microbial eukaryotes themselves than bacteria. Increased abundance of genes associated with unsaturated fatty acid biosynthesis likely plays a role in resisting high hydrostatic pressure. Top1 and hupB genes, responsible for the formation and stabilization of DNA structure, were unique and abundant in the hadal zone and thus may be helpful to stabilize DNA structure in the deep sea. Overall, our results provide insights into the distribution and potential adaptability of microbial eukaryotes in the hadal zone.

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“…Other less frequent fungal groups observed in this ecosystem were, Blastocladiomycota, LKM11, LKM15, Mucoromycota, and Zoopagomycota ( Figure 2B ). These results are consistent with earlier results obtained in deep-sea sediments from several parts of the planet confirming the general dominance of Ascomycota in deep-sea sediments together with the presence of Basidiomycota and Chytridiomycota in lower proportions (Li et al, 2016, 2019; Xu et al, 2016, 2019; Zhang et al, 2016; Nagano et al, 2017; Barone et al, 2018; Wang et al, 2019).…”

Section: Resultssupporting

confidence: 93%

Fungal communities in sediments along a depth gradient in the Eastern Tropical Pacific

Rojas-Jiménez

Grossart

Cordes

et al. 2020

Preprint

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Deep waters represent the largest biome on Earth and the largest ecosystem of Costa Rica. Fungi play a fundamental role in global biogeochemical cycling in marine sediments, yet, they remain little explored. We studied fungal diversity and community composition in several marine sediments from 16 locations sampled along a bathymetric gradient (from a depth of 380 to 3474 m) in two transects of about 1500 km length in the Eastern Tropical Pacific (ETP) of Costa Rica. Sequence analysis of the V7-V8 region of the 18S rRNA gene obtained from sediment cores revealed the presence of 787 fungal amplicon sequence variants (ASVs). On average, we detected a richness of 75 fungal ASVs per sample. Ascomycota represented the most abundant phylum with Saccharomycetes constituting the dominant class. Three ASVs accounted for ca. 63% of all fungal sequences: the yeast Metschnikowia (49.4%), Rhizophydium (6.9%), and Cladosporium (6.7%). Although we distinguished a cluster dominated by yeasts and a second cluster dominated by filamentous fungi, we were unable to detect a strong effect of depth, temperature, salinity, dissolved oxygen, and pH on the composition of fungal communities. We highlight the need to understand further the ecological role of fungi in deep-sea ecosystems.

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Fungal community analysis in seawater of the Mariana Trench as estimated by Illumina HiSeq

Abstract: Using Illumine Hiseq sequencing assessed the diversity and distribution of fungal communities in seawater of the Mariana Trench, with depth ranging 1000–4000 meters.

Cited by 21 publications

References 37 publications

Fungal Communities in Sediments Along a Depth Gradient in the Eastern Tropical Pacific

Fungal Communities in Sediments Along a Depth Gradient in the Eastern Tropical Pacific

Shift and Metabolic Potentials of Microbial Eukaryotic Communities Across the Full Depths of the Mariana Trench

Fungal communities in sediments along a depth gradient in the Eastern Tropical Pacific

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