Fungal cellulases: protein engineering and post-translational modifications

Engineering cellulases for conversion of lignocellulosic biomass

“…For the period before 2015, such studies are extensively reviewed in Payne et al (2015) . Recent reviews with more or less comprehensive overviews of more recent cellulase engineering studies date from 2020 ( Contreras et al , 2020a ) and 2021 ( Zhang et al , 2021 ). These include tables that list, and shortly describe, individual studies published, primarily, in the period of 2010–2020.…”

“…Fungal cellulases and their engineering have been reviewed by Payne et al (2015) in an impressively comprehensive review that addresses many (general) aspects of cellulase structure and function in much detail. Other useful reviews focusing on engineering cellulases include ( Bommarius et al , 2014 , Greene et al , 2015 , Contreras et al , 2020a , Zhang et al , 2021 ). In this short review, we discuss engineering strategies used for increasing the industrial performance of cellulases, presenting a few selected highlights from the past and recent advancements.…”

Engineering Enzymes for Environmental Sustainability

“…Hence, engineering individual lignocellulose deconstructing enzymes has been a topic of intense research. For instance, cellulases have been optimised for improved activity, thermostability and pH tolerance; the details of these efforts are outside the scope of this piece, but many of these efforts are covered in excellent reviews [86–89] . Engineered enzyme cocktails have also been developed, with multiple synergistic biocatalysts working together to deconstruct several biomass components in parallel [90] .…”

“…For instance, cellulases have been optimised for improved activity, thermostability and pH tolerance; the details of these efforts are outside the scope of this piece, but many of these efforts are covered in excellent reviews. [ 86 , 87 , 88 , 89 ] Engineered enzyme cocktails have also been developed, with multiple synergistic biocatalysts working together to deconstruct several biomass components in parallel. [90] These cocktails typically consist of cellulases, cellobiohydrolases, endoglucanases, β‐glucosidases, hemicellulases, and lytic polysaccharide monooxygenases.…”

Effect of cellulase on antioxidant activity and flavor of Rosa roxburghii Tratt

“…Cellulase acts on the β-1,4-glucosidic bond and is a multicomponent enzyme with multiple action sites ( Zhang, Cao, Bi, & Li, 2022 ). The enzymatic method has several advantages over chemical and physical methods, including low addition, low energy consumption, low pollution levels, and high safety ( de Souza & Kawaguti, 2021 ).…”