Fungal biotransformation of short-chain n-alkylcycloalkanes

Schlüter, Rabea; Dallinger, Anja; Kabisch, Jan; Duldhardt, Ilka; Schauer, Frieder

doi:10.1007/s00253-019-09749-4

Cited by 7 publications

(4 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Scheffersomyces stipitis is also intriguing in the bioethanol space for being a natural xylose fermenter (Song et al, 2022 ). On the other hand, while C. maltosa has been studied for potential applications involving utilization of phenols and alkanes, it remains undercharacterized relative to Y. lipolytica, S. stipitis , and R. toruloides (Chrzanowski et al, 2008 ; Schlüter et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Survey of nonconventional yeasts for lipid and hydrocarbon biotechnology

Ocasio

Khalid

Truka

et al. 2022

Journal of Industrial Microbiology and Biotechnology

View full text Add to dashboard Cite

Non-conventional yeasts have an untapped potential to expand biotechnology and enable process development necessary for a circular economy. They are especially convenient for the field of lipid and hydrocarbon biotechnology because they offer faster growth than plants, easier scalability than microalgae and exhibit increased tolerance relative to some bacteria. The ability of industrial organisms to import and metabolically transform lipids and hydrocarbons are crucial in such applications. Here, we assessed the ability of 14 yeasts to utilize 18 model lipids and hydrocarbons from six functional groups and three carbon chain lengths. The studied strains covered 12 genera from nine families. Nine non-conventional yeast performed better than Saccharomyces cerevisiae, the most common industrial yeast. Rhodotorula toruloides, Candida maltosa, Scheffersomyces stipis and Yarowia lipolytica were observed to grow significantly better and on more types of lipid and lipid-molecules than other strains. They were all able to utilize mid to long-chain fatty acids, fatty alcohols, alkanes, alkenes and dicarboxylic acids, including 28 previously unreported substrates across the four yeasts. Interestingly, a phylogenetic analysis showed a short evolutionary distance between the R. toruloides and C. maltosa and S. stipitis, even though R. toruloides is classified under a different phylum. This work provides valuable insight into the lipid substrate range of non-conventional yeasts that can inform species selection decisions and viability of lipid feedstocks.

show abstract

Section: Resultsmentioning

confidence: 99%

Survey of nonconventional yeasts for lipid and hydrocarbon biotechnology

Ocasio

Khalid

Truka

et al. 2022

Journal of Industrial Microbiology and Biotechnology

View full text Add to dashboard Cite

show abstract

“…Recently, special attention has been paid to the possibility of using fungi for bioremediation (mycoremediation). Fungi are able to survive in adverse environmental conditions as well as to transform pollutants that are inaccessible or hardly accessible to soil bacteria [ 10 , 11 , 12 , 13 ]. There are a number of works demonstrating the successful application of mycoremediation [ 14 , 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

The Effectiveness of Biostimulation, Bioaugmentation and Sorption-Biological Treatment of Soil Contaminated with Petroleum Products in the Russian Subarctic

et al. 2021

View full text Add to dashboard Cite

The effectiveness of different bioremediation methods (biostimulation, bioaugmentation, the sorption-biological method) for the restoration of soil contaminated with petroleum products in the Russian Subarctic has been studied. The object of the study includes soil contaminated for 20 years with petroleum products. By laboratory experiment, we established five types of microfungi that most intensively decompose petroleum hydrocarbons: Penicillium canescens st. 1, Penicillium simplicissimum st. 1, Penicillum commune, Penicillium ochrochloron, and Penicillium restrictum. One day after the start of the experiment, 6 to 18% of the hydrocarbons decomposed: at 3 days, this was 16 to 49%; at 7 days, 40 to 73%; and at 10 days, 71 to 87%. Penicillium commune exhibited the greatest degrading activity throughout the experiment. For soils of light granulometric composition with a low content of organic matter, a more effective method of bioremediation is sorption-biological treatment using peat or granulated activated carbon: the content of hydrocarbons decreased by an average of 65%, which is 2.5 times more effective than without treatment. The sorbent not only binds hydrocarbons and their toxic metabolites but is also a carrier for hydrocarbon-oxidizing microorganisms and prevents nutrient leaching from the soil. High efficiency was noted due to the biostimulation of the native hydrocarbon-oxidizing microfungi and bacteria by mineral fertilizers and liming. An increase in the number of microfungi, bacteria and dehydrogenase activity indicate the presence of a certain microbial potential of the soil and the ability of the hydrocarbons to produce biochemical oxidation. The use of the considered methods of bioremediation will improve the ecological state of the contaminated area and further the gradual restoration of biodiversity.

show abstract

“…As recently as 2019, Prenafeta-Boldú et al wrote that the literature on the fungal metabolism of alicyclic compounds is almost non-existent [ 18 ]. In addition to some previously described studies [ 14 , 19 , 27 , 74 ] and in order to complement research in this field, cyclohexanone was used as an isolation substrate in this study. The biodegradation capacity of the two F. oxysporum strains St4 and St5 was evaluated in the presence of cyclohexanone, which was degraded via ε-caprolactone, 6-hydroxyhexanoic and hexanedioic acid ( Figure 7 , pathway A) following the classical descriptions of bacterial cyclohexane degradation via cyclohexanone [ 75 ].…”

Section: Discussionmentioning

confidence: 99%

“…Cyclohexanol and cyclohexanone were detected as the only products with no further degradation of cyclohexanone. Methylcyclohexane and longer n -alkylcyclohexanes, on the other hand, can be completely degraded via side chain degradation, aromatization and aromatic ring cleavage reactions [ 19 , 74 ]. However, both Fusarium strains St4 and St5 presented in this work were able to metabolize cyclohexanone to ε-caprolactone demonstrating that cyclohexanone is not a dead-end product.…”

Section: Discussionmentioning

confidence: 99%

Comparative Bioremediation of Tetradecane, Cyclohexanone and Cyclohexane by Filamentous Fungi from Polluted Habitats in Kazakhstan

Gaid,

Jentzsch,

Beermann

et al. 2024

JoF

View full text Add to dashboard Cite

Studying the fates of oil components and their interactions with ecological systems is essential for developing comprehensive management strategies and enhancing restoration following oil spill incidents. The potential expansion of Kazakhstan’s role in the global oil market necessitates the existence of land-specific studies that contribute to the field of bioremediation. In this study, a set of experiments was designed to assess the growth and biodegradation capacities of eight fungal strains sourced from Kazakhstan soil when exposed to the hydrocarbon substrates from which they were initially isolated. The strains were identified as Aspergillus sp. SBUG-M1743, Penicillium javanicum SBUG-M1744, SBUG-M1770, Trichoderma harzianum SBUG-M1750 and Fusarium oxysporum SBUG-1746, SBUG-M1748, SBUG-M1768 and SBUG-M1769 using the internal transcribed spacer (ITS) region. Furthermore, microscopic and macroscopic evaluations agreed with the sequence-based identification. Aspergillus sp. SBUG-M1743 and P. javanicum SBUG-M1744 displayed remarkable biodegradation capabilities in the presence of tetradecane with up to a 9-fold biomass increase in the static cultures. T. harzianum SBUG-M1750 exhibited poor growth, which was a consequence of its low efficiency of tetradecane degradation. Monocarboxylic acids were the main degradation products by SBUG-M1743, SBUG-M1744, SBUG-M1750, and SBUG-M1770 indicating the monoterminal degradation pathway through β-oxidation, while the additional detection of dicarboxylic acid in SBUG-M1768 and SBUG-M1769 cultures was indicative of the fungus’ ability to undertake both monoterminal and diterminal degradation pathways. F. oxysporum SBUG-M1746 and SBUG-M1748 in the presence of cyclohexanone showed a doubling of the biomass with the ability to degrade the substrate almost completely in shake cultures. F. oxysporum SBUG-M1746 was also able to degrade cyclohexane completely and excreted all possible metabolites of the degradation pathway. Understanding the degradation potential of these fungal isolates to different hydrocarbon substrates will help in developing effective bioremediation strategies tailored to local conditions.

show abstract

Fungal biotransformation of short-chain n-alkylcycloalkanes

Cited by 7 publications

References 66 publications

Survey of nonconventional yeasts for lipid and hydrocarbon biotechnology

Survey of nonconventional yeasts for lipid and hydrocarbon biotechnology

The Effectiveness of Biostimulation, Bioaugmentation and Sorption-Biological Treatment of Soil Contaminated with Petroleum Products in the Russian Subarctic

Comparative Bioremediation of Tetradecane, Cyclohexanone and Cyclohexane by Filamentous Fungi from Polluted Habitats in Kazakhstan

Contact Info

Product

Resources

About