Fungal biotransformation of crude glycerol into malic acid

West, Thomas P.

doi:10.1515/znc-2015-0115

Cited by 20 publications

(6 citation statements)

References 12 publications

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“…It has been shown that crude glycerol derived from the production of biodiesel was capable of supporting fungal malic acid synthesis [1,2,30]. In a recent study, A. niger ATCC 9142, ATCC 10577 and ATCC 12846 were found to be capable of producing malic acid on 10% (v/v) crude glycerol after 192 h at 25 • C [34]. Of the three strains, A. niger ATCC 12846 produced the highest malic acid concentration from crude glycerol [34].…”

Section: Malic Acid Production From Biofuel-related Coproductsmentioning

confidence: 98%

“…In a recent study, A. niger ATCC 9142, ATCC 10577 and ATCC 12846 were found to be capable of producing malic acid on 10% (v/v) crude glycerol after 192 h at 25 • C [34]. Of the three strains, A. niger ATCC 12846 produced the highest malic acid concentration from crude glycerol [34]. The ability to produce malic acid from crude glycerol did not seem to be highly correlated with cellular biomass since ATCC 10577 produced a higher biomass level than did ATCC 12846 after 192 h at 25 • C [34].…”

Section: Malic Acid Production From Biofuel-related Coproductsmentioning

confidence: 99%

“…Of the three strains, A. niger ATCC 12846 produced the highest malic acid concentration from crude glycerol [34]. The ability to produce malic acid from crude glycerol did not seem to be highly correlated with cellular biomass since ATCC 10577 produced a higher biomass level than did ATCC 12846 after 192 h at 25 • C [34]. Although A. oryzae DSM 1863 was also capable of producing 45 g/L malic acid from 84 g/L (w/v) glycerol after 353 h at 35 • C, its ability to utilize crude glycerol as a substrate for malic acid production has not been investigated [19].…”

Section: Malic Acid Production From Biofuel-related Coproductsmentioning

confidence: 99%

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Microbial Production of Malic Acid from Biofuel-Related Coproducts and Biomass

West

2017

Fermentation

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The dicarboxylic acid malic acid synthesized as part of the tricarboxylic acid cycle can be produced in excess by certain microorganisms. Although malic acid is produced industrially to a lesser extent than citric acid, malic acid has industrial applications in foods and pharmaceuticals as an acidulant among other uses. Only recently has the production of this organic acid from coproducts of industrial bioprocessing been investigated. It has been shown that malic acid can be synthesized by microbes from coproducts generated during biofuel production. More specifically, malic acid has been shown to be synthesized by species of the fungus Aspergillus on thin stillage, a coproduct from corn-based ethanol production, and on crude glycerol, a coproduct from biodiesel production. In addition, the fungus Ustilago trichophora has also been shown to produce malic acid from crude glycerol. With respect to bacteria, a strain of the thermophilic actinobacterium Thermobifida fusca has been shown to produce malic acid from cellulose and treated lignocellulosic biomass. An alternate method of producing malic acid is to use agricultural biomass converted to syngas or biooil as a substrate for fungal bioconversion. Production of poly(β-L-malic acid) by strains of Aureobasidium pullulans from agricultural biomass has been reported where the polymalic acid is subsequently hydrolyzed to malic acid. This review examines applications of malic acid, metabolic pathways that synthesize malic acid and microbial malic acid production from biofuel-related coproducts, lignocellulosic biomass and poly(β-L-malic acid).

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Section: Malic Acid Production From Biofuel-related Coproductsmentioning

confidence: 98%

Section: Malic Acid Production From Biofuel-related Coproductsmentioning

confidence: 99%

Section: Malic Acid Production From Biofuel-related Coproductsmentioning

confidence: 99%

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Microbial Production of Malic Acid from Biofuel-Related Coproducts and Biomass

West

2017

Fermentation

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“…A. niger strains ATCC 9142 and ATCC 10577 were investigated for the production of malic acid from thin stillage, and achieved product titers of 17 and 19 g/L, respectively ( West, 2011 ). When using crude glycerol as feedstock, A. niger ATCC 12486 could produce 23 g/L malic acid after 192 h at 25°C ( West, 2015 ).…”

Section: Malic Acid Production Using Wild Type Microorganismsmentioning

confidence: 99%

Microbial Biosynthesis of L-Malic Acid and Related Metabolic Engineering Strategies: Advances and Prospects

Wei

et al. 2021

Front. Bioeng. Biotechnol.

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Malic acid, a four-carbon dicarboxylic acid, is widely used in the food, chemical and medical industries. As an intermediate of the TCA cycle, malic acid is one of the most promising building block chemicals that can be produced from renewable sources. To date, chemical synthesis or enzymatic conversion of petrochemical feedstocks are still the dominant mode for malic acid production. However, with increasing concerns surrounding environmental issues in recent years, microbial fermentation for the production of L-malic acid was extensively explored as an eco-friendly production process. The rapid development of genetic engineering has resulted in some promising strains suitable for large-scale bio-based production of malic acid. This review offers a comprehensive overview of the most recent developments, including a spectrum of wild-type, mutant, laboratory-evolved and metabolically engineered microorganisms for malic acid production. The technological progress in the fermentative production of malic acid is presented. Metabolic engineering strategies for malic acid production in various microorganisms are particularly reviewed. Biosynthetic pathways, transport of malic acid, elimination of byproducts and enhancement of metabolic fluxes are discussed and compared as strategies for improving malic acid production, thus providing insights into the current state of malic acid production, as well as further research directions for more efficient and economical microbial malic acid production.

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“…As global biodiesel production continues to increase, a surplus of the biodiesel coproduct crude glycerol has resulted and lowered the price of crude glycerol to about $0.01/pound [6]. A recent study has found that crude glycerol can be utilized by three strains of Aspergillus niger to produce malic acid [7]. Other fungi or yeast may also be able to utilize crude glycerol to synthesize malic acid.…”

Section: Introductionmentioning

confidence: 99%

Microbial Malic Acid Production: Exploring New Avenues of Synthesizing a Commercially-Valuable Chemical

West¹

2016

J Microb Biochem Technol

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Fungal biotransformation of crude glycerol into malic acid

Cited by 20 publications

References 12 publications

Microbial Production of Malic Acid from Biofuel-Related Coproducts and Biomass

Microbial Production of Malic Acid from Biofuel-Related Coproducts and Biomass

Microbial Biosynthesis of L-Malic Acid and Related Metabolic Engineering Strategies: Advances and Prospects

Microbial Malic Acid Production: Exploring New Avenues of Synthesizing a Commercially-Valuable Chemical

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