Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials

Sørensen, Annette; Lübeck, Mette; Lübeck, Peter Stephensen; Ahring, Birgitte Kiær

doi:10.3390/biom3030612

Cited by 220 publications

(156 citation statements)

References 116 publications

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“…β-Glucosidase is utilized in hydrolysis of cellobiose and short oligosaccharides to glucose during cellulose hydrolysis so that glucose can be fermented to ethanol or other biofuel [13,28,128]. Currently, T. reesei is the major source of the commercial cellulase enzyme used for cellulose hydrolysis and biofuel production but it lacks sufficient β-glucosidase activity therefore supplementation of exogenous β-glucosidase from other filamentous fungi such as Aspergillus niger is mandatory [28].…”

Section: Applications Based On Hydrolytic Activitymentioning

confidence: 99%

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

Ahmed¹,

Aslam²,

Ashraf³

et al. 2017

JAEM

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Cellulose is the most abundant biomaterial in the biosphere and the major component of plant biomass.Cellulase is an enzymatic system required for conversion of renewable cellulose biomass into free sugar for subsequent use in different applications. Cellulase system mainly consists of three individual enzymes namely: endoglucanase, exoglucanase and β-glucosidases. β-Glucosidases are ubiquitous enzymes found in all living organisms with great biological significance. β-Glucosidases have also tremendous biotechnological applications such as biofuel production, beverage industry, food industry, cassava detoxification and oligosaccharides synthesis. Microbial β-glucosidases are preferred for industrial uses because of robust activity and novel properties exhibited by them. This review aims at describing the various biochemical methods used for screening and evaluating β-glucosidases activity from microbial sources. Subsequently, it generally highlights techniques used for purification of β-glucosidases. It then elaborates various biochemical and molecular properties of this valuable enzyme such as pH and temperature optima, glucose tolerance, substrate specificity, molecular weight, and multiplicity. Furthermore, it describes molecular cloning and expression of bacterial, fungal and metagenomic β-glucosidases. Finally, it highlights the potential biotechnological applications of β-glucosidases.

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Section: Applications Based On Hydrolytic Activitymentioning

confidence: 99%

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

Ahmed¹,

Aslam²,

Ashraf³

et al. 2017

JAEM

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“…β-glucosidases play an important role in complex biomass hydrolysis from renewable sources for the production of biofuels, food, or food additives [112,113]. However, product inhibition impairing yields, thermal inactivation of enzymes, and the high cost of enzyme production are still the main obstacles to commercial hydrolysis processes.…”

Section: Iridoid De Novo Synthesis: Late Stepsmentioning

confidence: 99%

Deciphering the route to cyclic monoterpenes in Chrysomelina leaf beetles: source of new biocatalysts for industrial application?

Burse

Boland

2017

Zeitschrift Für Naturforschung C

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Abstract:The drastic growth of the population on our planet requires the efficient and sustainable use of our natural resources. Enzymes are indispensable tools for a wide range of industries producing food, pharmaceuticals, pesticides, or biofuels. Because insects constitute one of the most species-rich classes of organisms colonizing almost every ecological niche on earth, they have developed extraordinary metabolic abilities to survive in various and sometimes extreme habitats. Despite this metabolic diversity, insect enzymes have only recently generated interest in industrial applications because only a few metabolic pathways have been sufficiently characterized. Here, we address the biosynthetic route to iridoids (cyclic monoterpenes), a group of secondary metabolites used by some members of the leaf beetle subtribe Chrysomelina as defensive compounds against their enemies. The ability to produce iridoids de novo has also convergently evolved in plants. From plant sources, numerous pharmacologically relevant structures have already been described. In addition, in plants, iridoids serve as building blocks for monoterpenoid indole alkaloids with broad therapeutic applications. As the commercial synthesis of iridoidbased drugs often relies on a semisynthetic approach involving biocatalysts, the discovery of enzymes from the insect iridoid route can account for a valuable resource and economic alternative to the previously used enzymes from the metabolism of plants. Hence, this review illustrates the recent discoveries made on the steps of the iridoid pathway in Chrysomelina leaf beetles. The findings are also placed in the context of the studied counterparts in plants and are further discussed regarding their use in technological approaches.

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“…Issue 7,Vol.6 (October-November 2017) Available online on http://www.rspublication.com/ijeted/ijeted_index.htm ISSN 2249-6149 depletion of these fossil resources from non-renewable energy sources [2]. According to Gu, et al [3], studies have been carried out to find renewable energy sources as fossil fuel replacement.…”

Section: International Journal Of Emerging Trends In Engineering and mentioning

confidence: 99%

Biogas Production using Sugarcane Bagasse

Boamah¹,

Armah²,

Tetteh³

2017

IJETED

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Non-renewable energy sources have been found to pose various environmental problems such as greenhouse gas emissions as they tend to deplete at faster rates. Renewable energy could, however, replace the conventional sources of energy such as fossil fuel and oil, serving as an alternative source of energy. Biological processes such as fermentation and anaerobic digestion for the past decades have given rise to the production of biofuels such as biogas and bioethanol. The inchoate gain in the shift for a renewable source of energy is that the feedstock is often a by-product, a residue or waste product of other processes without the competition of arable land. One of such feedstock is sugarcane bagasse. Biochemical methane potential (BMP) test is generally used to determine the possible methane that can be obtained from feedstocks. This study, however,aims at optimizing the anaerobic digestion of sugarcane bagasse with cow dungin a BMP test and controlled at mesophilic temperature (35 ± 2). Biodigester labelled as 1A (containing only the inoculum) was used as the control for the experiment, biodigester 2A (with 1:1 feedstock to inoculum ratio), biodigester 1C (without purification but a 1:3 feedstock to inoculum ratio) and biodigester 1D (with purification after a 1:3 feedstock to inoculum ratio) were reported in this study. Methane production was measured for a retention time (days) of 21 days using a 1000ml Schott bottles as biodigesters in batch mode. Sugarcanebagassewas characterized in the batch reactor to enable the inoculum activity and the biogas volume reported during the 21 days. The highest yield of methane was found to be 78% whiles the cumulative average biogas yield was 167mlfor the 21 days. However, the use of chemical absorption techniques for carbon dioxide removal in anaerobic digestion for biogas production is recommended as a promising factor.

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Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials

Cited by 220 publications

References 116 publications

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

Deciphering the route to cyclic monoterpenes in Chrysomelina leaf beetles: source of new biocatalysts for industrial application?

Biogas Production using Sugarcane Bagasse

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