Fungal and Bacterial Diversity in the Tuber magnatum Ecosystem and Microbiome

Marozzi, Giorgio; Benucci, Gian Maria Niccolò; Turchetti, Benedetta; Massaccesi, Luisa; Leonardo, Baciarelli Falini; Bonito, Gregory; Buzzini, Pietro; Agnelli, Alberto; Donnini, Domizia; Albertini, Emidio

doi:10.1007/s00248-021-01950-1

Cited by 7 publications

(4 citation statements)

References 90 publications

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“…Most of the known fungi of the genus Plectosphaerella were isolated from soils (Soledad et al 2023). They are pathogens of several plant species and are described as a component of the core fungal genera associated with forests producing white truffles (Giorgio et al 2023). However, previously, representatives of Plectosphaerella were never reported as organisms living inside the ascomata of truffles, and we found no mention of these fungi as pathogens of truffles, especially black truffles.…”

Section: Genus Of Bacteriacontrasting

confidence: 68%

First Report on Truffle-Inhabiting Fungi and Metagenomic Communities of Tuber Aestivum Collected in Russia

Malygina,

Imidoeva,

Morgunova

et al. 2024

J Exp Bio & Ag Sci

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Truffles are one of the least studied groups of fungi in terms of their biological and biotechnological aspects. This study aimed to isolate truffle-inhabiting fungi and assess the metagenomic communities of the most common Russian summer truffle, Tuber aestivum. This study is the first to characterize the biodiversity of prokaryotic and eukaryotic organisms living in the truffle T. aestivum using molecular analysis and sequencing. Plant pathogens involved in a symbiotic relationship with truffles were identified by sequencing the hypervariable fragments of the 16S rRNA and 18S rRNA genes. In addition, some strains of fungal symbionts and likely pathogens were isolated and recognized for the first time from the truffles. This study also compared and characterized the general diversity and distribution of microbial taxa of T. aestivum collected in Russia and Europe. The results revealed that the Russian and European truffle study materials demonstrated high similarity. In addition to the truffles, representatives of bacteria, fungi, and protists were found in the fruiting bodies. Many of these prokaryotic and eukaryotic species inhabiting truffles might influence them, help them form mycorrhizae with trees, and regulate biological processes. Thus, truffles are interesting and promising sources for modern biotechnological and agricultural studies.

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Section: Genus Of Bacteriacontrasting

confidence: 68%

First Report on Truffle-Inhabiting Fungi and Metagenomic Communities of Tuber Aestivum Collected in Russia

Malygina,

Imidoeva,

Morgunova

et al. 2024

J Exp Bio & Ag Sci

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“…Soil samples were taken from a depth of approximately 5-10 cm around the rhizomes, and previous research has shown that comparing depths of 0 cm, 40 cm, 100 cm, and 200 cm revealed no detectable differences in α-diversity or β-diversity. This suggests that the impact of PCH on the soil microbial community is minimal, allowing us to conclude that depth and microbes are not strongly correlated in this experiment (Giorgio et al, 2023). Many microorganisms in the soil and rhizomes exhibit statistical coexistence.…”

Section: Discussionmentioning

confidence: 77%

Quality analysis and function prediction of soil microbial communities of Polygonatum cyrtonema in two indigenous-origins

Yang,

Wulu

et al. 2024

Front. Microbiol.

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Polygonatum cyrtonema Hua (PCH), as an important economic crop, is used as raw industrial materials and traditional Chinese medicine. There are significant variations in the quality of PCH from different geographical origins. It can be due to the change of the endophytic fungi and soil microbial communities of PCH. Therefore, the aim of this study is to investigate the composition and functional prediction of the main microbial communities in the rhizomes and soil of PCH and explore their impact on medicinal quality. High-throughput sequencing techniques targeting ITS and 16S rDNA were employed to compare the structure and biodiversity differences of endophytic fungi in the rhizomes and soil microbial communities of PCH from 12 different locations in Sichuan and Guangxi province. Heatmap analysis was used for comprehensive statistics and visualization of the richness of rhizome and soil microbial communities from all locations. Venn analysis was conducted to determine the total number of shared fungi between rhizomes and soil, and GraphPad Prism analysis was employed to predict and compare the microbial communities related to phenotypes at the genus level in Sichuan and Guangxi. Tax4Fun and Fungild were used for metabolic function prediction of microbial communities in the rhizomes and soil of PCH. The results revealed the identification of 19,387 bacterial amplicon sequence variants (ASVs) in the rhizomes and 37,990 bacterial ASVs in the soil, with 6,889 shared bacterial ASVs. In addition, 2,948 fungal ASVs were identified in the rhizomes and 8,868 in the soil, with 1,893 shared fungal ASVs. Microbial sequencing results indicated that the fungal communities between soil and rhizomes were mainly composed of Ascomycota and Basidiomycota, while bacterial communities included Proteobacteria, Acidobacteria, Bacteroidota, Gammatimonadota, and Firmicutes. Dominant bacterial groups such as Nitrospira, Acidibacter, and fungal groups including Mortierella, Ceratobasidium, and Fusarium were identified as potential contributors to the observed traits. In the top 15 microbial genera, both Sichuan and Guangxi contain 15 bacterial genera, but there are differences in their abundance. Guangxi has three unique fungal genera, including the genera Scleroderma, Russula, and Gliocladiopsis. On the other hand, Sichuan has the unique fungal genus Chamaeota. The correlation analysis between the microbiota and the chemical content from 12 different collecting spots was performed by GraphPad Prism. Burkholderia-Caballeronia-Paraburkholderia, Acidibacter, and Amycolatopsis show an inverse proportionality to total polysaccharides and saponins, while Enterobacter shows a direct proportionality to total polysaccharides and inverse proportionality to saponins. The metabolism pathways show a significant positive correlation with PCH polysaccharides and saponins. This study provide new insights into the mechanisms underlying the quality differences between the two major indigenous areas.

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“…Remarkably, the bacterial diversity within the truffles was minimal, ranging from 2–23 OTUs, with just a single Bradyrhizobium OTU predominant among Tuber fruit bodies, regardless of geographical provenance, and not found in the other truffle genera examined [ 64 ]. Clearly, we found a much more diverse bacterial community [ 30 , 34 , 39 , 65 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

The Microbiome Structure of the Symbiosis between the Desert Truffle Terfezia boudieri and Its Host Plant Helianthemum sessiliflorum

Satish

Barak

Keren

et al. 2022

JoF

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The desert truffle Terfezia boudieri is an ascomycete fungus that forms ect-endomycorrhiza in the roots of plants belonging to Cistaceae. The fungus forms hypogeous edible fruit bodies, appreciated as gourmet food. Truffles and host plants are colonized by various microbes, which may contribute to their development. However, the diversity and composition of the bacterial community under field conditions in the Negev desert are still unknown. The overall goal of this research was to identify the rhizosphere microbial community supporting the establishment of a symbiotic association between T. boudieri and Helianthemum sessiliflorum. The bacterial community was characterized by fruiting bodies, mycorrhized roots, and rhizosphere soil. Based on next-generation sequencing meta-analyses of the 16S rRNA gene, we discovered diverse bacterial communities of fruit bodies that differed from those found in the roots and rhizosphere. Families of Proteobacteria, Planctomycetes, and Actinobacteria were present in all four samples. Alpha diversity analysis revealed that the rhizosphere and roots contain significantly higher bacterial species numbers compared to the fruit. Additionally, ANOSIM and PCoA provided a comparative analysis of the bacterial taxa associated with fruiting bodies, roots, and rhizosphere. The core microbiome described consists of groups whose biological role triggers important traits supporting plant growth and fruit body development.

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Fungal and Bacterial Diversity in the Tuber magnatum Ecosystem and Microbiome

Cited by 7 publications

References 90 publications

First Report on Truffle-Inhabiting Fungi and Metagenomic Communities of Tuber Aestivum Collected in Russia

First Report on Truffle-Inhabiting Fungi and Metagenomic Communities of Tuber Aestivum Collected in Russia

Quality analysis and function prediction of soil microbial communities of Polygonatum cyrtonema in two indigenous-origins

The Microbiome Structure of the Symbiosis between the Desert Truffle Terfezia boudieri and Its Host Plant Helianthemum sessiliflorum

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