Fungal and Bacterial Communities in Tuber melanosporum Plantations from Northern Spain

Aza, Celia Herrero de; Armenteros, Sergio; McDermott, James; Mauceri, Stefano; Olaizola, Jaime; Hernández-Rodríguez, Mónica; Mediavilla, Olaya

doi:10.3390/f13030385

Cited by 12 publications

(6 citation statements)

References 73 publications

(92 reference statements)

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“…In detail, significant promotion was detected using the M2 formulation based on Pseudomonas sp. Recently, a comprehensive study of the microbiomes in truffle orchards showed that the Pseudomonas and Bradyrhizobium genera, which are known as “helper” bacteria, were present in high numbers [ 43 , 44 ]. Previous works indicated that these bacteria are able to promote root mycorrhization [ 36 ] and play a key role in establishing plant–fungal symbiosis [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Bacteria Inoculation on Colonization of Roots by Tuber melanosporum and Growth of Quercus ilex Seedlings

Giorgi,

Amicucci,

Landi

et al. 2024

Plants

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Tuber melanosporum is an ascomycete that forms ectomycorrhizal (ECM) symbioses with a wide range of host plants, producing edible fruiting bodies with high economic value. The quality of seedlings in the early symbiotic stage is important for successful truffle cultivation. Numerous bacterial species have been reported to take part in the truffle biological cycle and influence the establishment of roots symbiosis in plant hosts and the development of the carpophore. In this work, three different bacteria formulations were co-inoculated in Quercus ilex L. seedlings two months after T. melanosporum inoculation. At four months of bacterial application, the T. melanosporum ECM root tip rate of colonization and bacterial presence were assessed using both morphological and molecular techniques. A 2.5-fold increase in ECM colonization rate was found in the presence of Pseudomonas sp. compared to the seedlings inoculated only with T. melanosporum. The same treatment caused reduced plant growth either for the aerial and root part. Meanwhile, the ECM colonization combined with Bradyrhizobium sp. and Pseudomonas sp. + Bradyrhizobium sp. reduced the relative density of fibrous roots (nutrient absorption). Our work suggests that the role of bacteria in the early symbiotic stages of ECM colonization involves both the mycorrhizal symbiosis rate and plant root development processes, both essential for improve the quality of truffle-inoculated seedlings produced in commercial nurseries.

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Section: Discussionmentioning

confidence: 99%

Effect of Bacteria Inoculation on Colonization of Roots by Tuber melanosporum and Growth of Quercus ilex Seedlings

Giorgi,

Amicucci,

Landi

et al. 2024

Plants

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“…Microorganisms that are symbiotic with truffles are understudied, and their biological and biomedical potential has not been described. Several studies on the composition of truffle-associated microbial communities have assessed truffles collected in European countries (Vahdatzadeh et al 2019;Monaco et al 2020;Siebyła andSzyp-Borowska 2022;Herero de Aza et al 2022). However, in Russia, studies on this topic are now being reported for the first time.…”

Section: Discussionmentioning

confidence: 99%

First Report on Truffle-Inhabiting Fungi and Metagenomic Communities of Tuber Aestivum Collected in Russia

Malygina,

Imidoeva,

Morgunova

et al. 2024

J Exp Bio & Ag Sci

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Truffles are one of the least studied groups of fungi in terms of their biological and biotechnological aspects. This study aimed to isolate truffle-inhabiting fungi and assess the metagenomic communities of the most common Russian summer truffle, Tuber aestivum. This study is the first to characterize the biodiversity of prokaryotic and eukaryotic organisms living in the truffle T. aestivum using molecular analysis and sequencing. Plant pathogens involved in a symbiotic relationship with truffles were identified by sequencing the hypervariable fragments of the 16S rRNA and 18S rRNA genes. In addition, some strains of fungal symbionts and likely pathogens were isolated and recognized for the first time from the truffles. This study also compared and characterized the general diversity and distribution of microbial taxa of T. aestivum collected in Russia and Europe. The results revealed that the Russian and European truffle study materials demonstrated high similarity. In addition to the truffles, representatives of bacteria, fungi, and protists were found in the fruiting bodies. Many of these prokaryotic and eukaryotic species inhabiting truffles might influence them, help them form mycorrhizae with trees, and regulate biological processes. Thus, truffles are interesting and promising sources for modern biotechnological and agricultural studies.

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“…Mycorrhization helper bacteria (MHB) are widespread around the roots of different plant–fungal mycorrhizae. Past literature has suggested that Agrobacterium [ 28 ], Burkholderia [ 29 ], Pseudomonas [ 30 ], Bacillus [ 31 ], Paenibacillus [ 32 ], and the actinomycete Streptomyces [ 33 ] are mycorrhization helper bacteria. In our findings, the mycorrhizal rhizosphere exhibited a lower Simpson index and higher Shannon and ACE indices ( Table 1 ), which indicated that this area possessed higher soil bacterial diversity and evenness.…”

Section: Discussionmentioning

confidence: 99%

Responses of Bacterial Community Structure, Diversity, and Chemical Properties in the Rhizosphere Soil on Fruiting-Body Formation of Suillus luteus

et al. 2022

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Mycorrhiza helper bacteria (MHB) play an important role in driving mycorrhizal formation. There are few reports on the relationship between bacteria and fruiting growths. Taking mycorrhizal rhizosphere soil from sporocarps of the S. luteus and non-mycorrhizal rhizosphere soil of the host plant (Larix gmelinii), we measured the bacterial community structure and diversity and chemical properties to clarify the effect of bacteria on fruiting-body formation. The bacterial diversity was significantly higher in mycorrhizal rhizosphere soil (p < 0.05) than that in non-mycorrhizal rhizosphere soil. The relative abundance of Burkholderia, Bradyrhizobium, Pseudomonas, and Rhizobium was significantly higher (p < 0.05) in mycorrhizal rhizosphere soil than in non-mycorrhizal rhizosphere soil. The soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), ammonium nitrogen (AN), available phosphorus (AP), available potassium (AK), and the activity of catalase, urease, and phosphatase in mycorrhizal rhizosphere soil were significantly higher (p < 0.05) than those in non-mycorrhizal rhizosphere soil. A redundancy analysis (RDA) showed that dominant bacteria are closely related to soil enzyme activity and physicochemical properties (p < 0.05). The boletus recruits a large number of bacteria around the plant roots that speed up nutrient transformation and increase the soil nutrient content, providing an important guarantee for mycelium culture and fruiting-body formation. These findings provide ideas for the nutritional supply of boletus sporocarps and lay the theoretical foundation for the efficient artificial cultivation of boletus.

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Fungal and Bacterial Communities in Tuber melanosporum Plantations from Northern Spain

Cited by 12 publications

References 73 publications

Effect of Bacteria Inoculation on Colonization of Roots by Tuber melanosporum and Growth of Quercus ilex Seedlings

Effect of Bacteria Inoculation on Colonization of Roots by Tuber melanosporum and Growth of Quercus ilex Seedlings

First Report on Truffle-Inhabiting Fungi and Metagenomic Communities of Tuber Aestivum Collected in Russia

Responses of Bacterial Community Structure, Diversity, and Chemical Properties in the Rhizosphere Soil on Fruiting-Body Formation of Suillus luteus

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