This study analyses the effects of the 'Fostering Leading Universities for Start-ups' (FLUS) project, which is one of the Korean government's support programmes for nurturing entrepreneurial universities. This study uses the difference-in-differences (DID) methodology based on the fixed-effects model and the random-effects model with panel data. The results show that the net effects of the FLUS project were not directly significant on the universities' entrepreneurial culture. However, they positively contributed to the universities' entrepreneurial activities and performance concerning sales and job creation through start-ups. Thus, this study could provide implications for establishing university start-up policies in countries.