Fundamentals of In Situ Tissue Regeneration

Lee, S.J.; Yoo, James J.; Atala, Anthony

doi:10.1016/b978-0-12-802225-2.00001-5

Cited by 16 publications

(50 citation statements)

References 92 publications

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“…This process is aided through the exploitation of a tissue-specific scaffold that can control the microenvironment at the injured site, that will enable the mobilization of host stem/progenitor cells to these tissues and ensures their subsequent proliferation [19]. In doing so, it eliminates the need for in vitro cell manipulation and offers an alternative therapy path that decreases the time, and resources required to successfully regenerate a tissue or organ [21,26]. It takes advantage of the natural presence of the biochemical and biophysical cues at the injured site of the living tissue by employing the living body as a more effective bioreactor that can regenerate and maintain new tissue at the site of injury in a scalable and cost-effective way [25].…”

Section: From In Vitro To In Situmentioning

confidence: 99%

Biomaterials for In Situ Tissue Regeneration: A Review

2019

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This review focuses on a somewhat unexplored strand of regenerative medicine, that is in situ tissue engineering. In this approach manufactured scaffolds are implanted in the injured region for regeneration within the patient. The scaffold is designed to attract cells to the required volume of regeneration to subsequently proliferate, differentiate, and as a consequence develop tissue within the scaffold which in time will degrade leaving just the regenerated tissue. This review highlights the wealth of information available from studies of ex-situ tissue engineering about the selection of materials for scaffolds. It is clear that there are great opportunities for the use of additive manufacturing to prepare complex personalized scaffolds and we speculate that by building on this knowledge and technology, the development of in situ tissue engineering could rapidly increase. Ex-situ tissue engineering is handicapped by the need to develop the tissue in a bioreactor where the conditions, however optimized, may not be optimum for accelerated growth and maintenance of the cell function. We identify that in both methodologies the prospect of tissue regeneration has created much promise but delivered little outside the scope of laboratory-based experiments. We propose that the design of the scaffolds and the materials selected remain at the heart of developments in this field and there is a clear need for predictive modelling which can be used in the design and optimization of materials and scaffolds.

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Section: From In Vitro To In Situmentioning

confidence: 99%

Biomaterials for In Situ Tissue Regeneration: A Review

2019

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“…The use of natural and artificial 3D architectures as scaffold for as a support, with or without cells, is one of the most used strategies in tissue engineering [140,141]. Although this strategy has been validated and has provided relevant advances in the field, cell-based therapies have a limited pathway due to the availability of tissue from the donor.…”

Section: Biomaterials For Signalling Release Platforms: In Situ Tissumentioning

confidence: 99%

Instructive microenvironments in skin wound healing: Biomaterials as signal releasing platforms

Castaño

Pérez‐Amodio

Nieva

et al. 2018

Advanced Drug Delivery Reviews

150

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Skin wound healing aims to repair and restore tissue through a multistage process that involves different cells and signalling molecules that regulate the cellular response and the dynamic remodelling of the extracellular matrix. Nowadays, several therapies that combine biomolecule signals (growth factors and cytokines) and cells are being proposed. However, a lack of reliable evidence of their efficacy, together with associated issues such as high costs, a lack of standardization, no scalable processes, and storage and regulatory issues, are hampering their application. In situ tissue regeneration appears to be a feasible strategy that uses the body's own capacity for regeneration by mobilizing host endogenous stem cells or tissue-specific progenitor cells to the wound site to promote repair and regeneration. The aim is to engineer instructive systems to regulate the spatio-temporal delivery of proper signalling based on the biological mechanisms of the different events that occur in the host microenvironment. This review describes the current state of the different signal cues used in wound healing and skin regeneration, and their combination with biomaterial supports to create instructive microenvironments for wound healing.

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“…Regardless of the in situ fabrication strategy used, biomaterials have a key role in the regeneration process. Initially, in situ regeneration approaches focused on cell recruitment to the injury site through biomaterials and/or biological cues (Ko et al, 2013;Lee et al, 2016). However, it is well known that a non-target-specific scaffolding system lacks with the formation functional tissue .…”

Section: Materials For In Situ Tissue Regenerationmentioning

confidence: 99%

“…Commonly, in situ biomaterials are categorized as natural biomaterials, synthetic polymers, bioceramics, and ECMbased materials Murdock and Stephen, 2017). Natural biomaterials including polysaccharides (such as cellulose, alginate, hyaluronic acid, starch, dextran, heparin, chitin, and chitosan) and proteins (collagen, gelatin, and fibrin) have been broadly used in in situ regeneration due to their ECM similarity and recognition sites Lee et al, 2016). Contrary to natural biomaterials, synthetic polymers present good mechanical properties and can be manufactured easily with high precision Murdock and Stephen, 2017).…”

Section: Materials For In Situ Tissue Regenerationmentioning

confidence: 99%

“…Despite ECM-based materials can be derived from decellularized tissues that are similar to the native tissue present as major limitation the donor shortage (Crapo et al, 2011;Li et al, 2015). Nevertheless, due to the technological advances, it is possible to create an ECM-based materials that mimic the native tissues in terms of its architecture and biomechanical properties and allows cells and biomolecules to be incorporated Lee et al, 2016). It is important to highlight that in most of the in situ approaches the use of biomaterials is required (regardless of their shape or size), either being by injection, surgery, or insertion into the defect.…”

Section: Materials For In Situ Tissue Regenerationmentioning

confidence: 99%

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