Fundamentals and exploration of aggregation-induced emission molecules for amyloid protein aggregation

Abstract

The past decade has witnessed the growing interest and advances in aggregation-induced emission (AIE) molecules as driven by their unique fluorescence/optical properties in particular sensing applications including biomolecule sensing/detection, environmental/health...

Biflavones inhibit the fibrillation and cytotoxicity of the human islet amyloid polypeptide

“…The ThT fluorescence assay was performed to observe the change of peptide aggregation in the presence of inhibitors. 42 As shown in Fig. 2, incubation of 5 μM hIAPP resulted in intense fluorescence due to the peptide self-assembly.…”

Deciphering the Structure and Formation of Amyloids in Neurodegenerative Diseases With Chemical Biology Tools

“…Historical fluorescent probes for the detection of amyloids (e.g., ThS, ThT, ANS, Bis-ANS, Congo Red, Nile Red…) ( Figure 8A ) ( Naiki et al, 1989 ; Biancalana and Koide, 2010 ; Krebs et al, 2005 ; Groenning, 2010 ; Amdursky et al, 2012 ) were recently complemented by a wide range of aggregation-induced emission (AIE) molecules ( Tang et al, 2022 ; Tang et al, 2021 ) including hexaphenylsilole (HPS), tetraphenylethylene (TPE) and tetraphenylbutadiene that expand the detection of aggregates formed along the amyloidogenic pathway such as oligomers that are poorly detected by ThT, Congo Red and their derivatives. AIE molecules usually possess twisted structures in solution limiting the fluorescence yield by non-radiative transition ( Kumar et al, 2017 ; Aliyan et al, 2019 ).…”

Conformational-specific self-assembled peptides as dual-mode, multi-target inhibitors and detectors for different amyloid proteins

“…Second, conventional design strategies of amyloid-binding peptides mainly aim to use amyloid fragments derived from their parent amyloid proteins as recognition agents to bind to their parent amyloid proteins for either detecting or inhibiting amyloid aggregation. For example, Ab 31-42 , Ab 39-42 , Ab 16-20 , Ab 17-21 , [18][19][20][21][22] and their analogues 23 demonstrated their inhibitory ability to prevent Ab formation, while hIAPP 20-25 and hIAPP [24][25][26][27][28][29] were identified as amyloid inhibitors to prevent fulllength hIAPP 1-37 aggregation and fibrillization. 24,25 Since these amyloid fragmental peptides are mainly derived from their parent proteins, it is not surprising that they can bind to the homologous sequences of the corresponding amyloid proteins and can interfere with the aggregation of their parent amyloid proteins, but not other amyloid proteins.…”