Fundamental Limits in Measuring the Anisotropic Rotational Diffusion of Single Molecules
Weiyan Zhou,
Tingting Wu,
Matthew D. Lew
Abstract:Many biophysical techniques, such as singlemolecule fluorescence correlation spectroscopy, Forster resonance energy transfer, and fluorescence anisotropy, measure the translation and rotation of biomolecules to quantify molecular processes at the nanoscale. These methods often simplify data analysis by assuming isotropic rotational diffusion, e.g., that molecules wobble within a circular cone. This simplification ignores the anisotropy present in many biological contexts that may cause molecules to exhibit dif… Show more
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