Experimental aerosol generation methods aim to represent natural processes; however, the complexity is not always captured and unforeseen variability may be introduced into the data. The current practices for natural and experimental aerosol generation techniques are reviewed here. Recommendations for best practice are presented, and include characterization of starting material and spray fluid, rational selection of appropriate aerosol generators, and physical and biological characterization of the output aerosol. Reporting of bioaerosol research should capture sufficient detail to aid data interpretation, reduce variation, and facilitate comparison between research laboratories. Finally, future directions and challenges in bioaerosol generation are discussed.