Fundamental differences between the nonlinearities of pattern and focal electroretinograms

Abstract

We directly compared nonlinear kernels of normal human pattern electroretinograms (PERGs) and corresponding localized flash ERGs (FERGs). The FERG was triphasic and resembled an adaptive process because it decayed slowly without changing shape over several kernel orders and interpulse intervals. The PERG was biphasic in the slice nearest the diagonal of the second-order kernel, similar to the FERG in slices farther from this diagonal, and without power in higher-order kernels. The unique PERG features were short-term effects that immediately followed a contrast transition. The appearance-disappearance PERG had a triphasic first-order kernel and a biphasic second-order kernel. The latter was similar to, but half the size of, that for the contrast-reversal PERG. When the first off-diagonal slices of the two PERG second-order kernels were analyzed in detail, we found in both that the first positive peak was larger than the FERG at intermediate spatial frequencies. Both PERG peaks in the slice had a low contrast threshold and were linear with contrast. The three FERG peaks of the corresponding FERG slice had a higher threshold and were saturated with increasing contrast. These observations show that the PERG contains substantial pattern specific nonlinear components and cannot be dismissed as merely the nonlinear subcomponents of the corresponding FERG.