Fundamental Aspects of Microbial Membrane Electroporation

Pagán, Rafael; Mañas, Pilar

doi:10.1007/978-0-387-31122-7_3

Cited by 16 publications

(14 citation statements)

References 80 publications

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“…As with pressure, the occurrence of sublethal injury in yeast after PEF has been shown to be independent of the treatment medium pH (54). The similarity in the effect of pH on inactivation and injury by HHP and PEF may perhaps be related to the fact that the cytoplasmic membrane is a major target for damage in both processes (40).…”

Section: Discussionmentioning

confidence: 99%

Relationship between Sublethal Injury and Microbial Inactivation by the Combination of High Hydrostatic Pressure and Citral or tert -Butyl Hydroquinone

Somolinos¹,

García²,

Pagán³

et al. 2008

Appl Environ Microbiol

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The aim was to investigate (i) the occurrence of sublethal injury in Listeria monocytogenes, Escherichia coli, and Saccharomyces cerevisiae after high hydrostatic pressure (HHP) treatment as a function of the treatment medium pH and composition and (ii) the relationship between the occurrence of sublethal injury and the inactivating effect of a combination of HHP and two antimicrobial compounds, tert-butyl hydroquinone (TBHQ) and citral. The three microorganisms showed a high proportion of sublethally injured cells (up to 99.99% of the surviving population) after HHP. In E. coli and L. monocytogenes, the extent of inactivation and sublethal injury depended on the pH and the composition of the treatment medium, whereas in S. cerevisiae, inactivation and sublethal injury were independent of medium pH or composition under the conditions tested. TBHQ alone was not lethal to E. coli or L. monocytogenes but acted synergistically with HHP and 24-h refrigeration, resulting in a viability decrease of >5 log 10 cycles of both organisms. The antimicrobial effect of citral depended on the microorganism and the treatment medium pH. Acting alone for 24 h under refrigeration, 1,000 ppm of citral caused a reduction of 5 log 10 cycles of E. coli at pH 7.0 and almost 3 log 10 cycles of L. monocytogenes at pH 4.0. The combination of citral and HHP also showed a synergistic effect. Our results have confirmed that the detection of sublethal injury after HHP may contribute to the identification of those treatment conditions under which HHP may act synergistically with other preserving processes.

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Section: Discussionmentioning

confidence: 99%

Relationship between Sublethal Injury and Microbial Inactivation by the Combination of High Hydrostatic Pressure and Citral or tert -Butyl Hydroquinone

Somolinos¹,

García²,

Pagán³

et al. 2008

Appl Environ Microbiol

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show abstract

“…Electric field induces a trans-membrane potential difference across the cell membranes. When this potential difference reaches a critical value of 1 V, localised breakdown occurs, increasing cell membrane permeability (Pagán & Mañas, 2006). Therefore, the increment of the permeability of the grape skin cells by PEF processing seems to facilitate the extraction of polyphenols from the skins during maceration.…”

Section: Evolution Of Folin-ciocalteu Index and Colour Intensity Durimentioning

confidence: 99%

Evolution of polyphenolic compounds in red wine from Cabernet Sauvignon grapes processed by pulsed electric fields during aging in bottle

et al. 2010

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“…The induced damage in a microbial population after a treatment can be due to the loss of integrity and/or functionality of their cellular envelopments (Pagán & Mañas, 2006). The existence of sublethal injury after the treatment is usually determined by pour plating samples on two different media; a non-selective media with suitable conditions, where cells may return to its initial native state by repairing the cellular damage; and a selective media, where injured cells cannot be repaired, as a consequence, those cells die (Mackey, 2000).…”

Section: Membrane Damage Of Salmonella Typhimurium and S Aureus Cellmentioning

confidence: 99%

Inactivation of Salmonella Typhimurium and Staphylococcus aureus by pulsed electric fields in liquid whole egg

Monfort

Gayán

Saldaña

et al. 2010

Innovative Food Science & Emerging Technologies

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Fundamental Aspects of Microbial Membrane Electroporation

Cited by 16 publications

References 80 publications

Relationship between Sublethal Injury and Microbial Inactivation by the Combination of High Hydrostatic Pressure and Citral or tert -Butyl Hydroquinone

Relationship between Sublethal Injury and Microbial Inactivation by the Combination of High Hydrostatic Pressure and Citral or tert -Butyl Hydroquinone

Evolution of polyphenolic compounds in red wine from Cabernet Sauvignon grapes processed by pulsed electric fields during aging in bottle

Inactivation of Salmonella Typhimurium and Staphylococcus aureus by pulsed electric fields in liquid whole egg

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