Functions of the tegument of schistosomes: Clues from the proteome and lipidome

Hellemond, Jaap J. van; Retra, Kim; Brouwers, Jos F.; Balkom, Bas W. M. van; Yazdanbakhsh, Maria; Shoemaker, Charles B.; Tielens, Aloysius G.M.

doi:10.1016/j.ijpara.2006.01.007

Cited by 160 publications

(122 citation statements)

References 70 publications

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“…The blebbing of the worms when treated with bitter cucumber extracts ( Figure 2I) was the same as that of Haplorchis taichui treated with A. lakoocha Roxb. [12] and the effect of praziquantel on Fibricola seoulensis [13]. The functional roles of the tegumental surface are for attachment and nutrient uptake from the host [14].…”

Section: Discussionmentioning

confidence: 99%

In vitro Effects of Some Thai Anthelmintic Plants on Mortality and Change of Tegumental Surface of Stellantchasmus falcatus

Buddhachat¹,

Chantima²

2012

J Bacteriol Parasitol

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IntroductionStellantchasmus falcatus, a species of fluke in the Family Heterophyidae, is a minute intestinal trematode of fish-eating birds and mammals. Human infections with this fluke have been reported in Hawaii [1], Philippines [2], Japan [3], Thailand [4] and Korea [5]. The S. falcatus metacercariae or infective stage is commonly found in freshwater fish such as Mugil sp. [5], Xenetodon cancila [6] and Dermogenus pusillus [7]. In Thailand, five species of flukes i.e. Haplorchis pummilio, H. taichui, H. yokogawai, S. falcatus and Centrocestus caninus were found responsible for human infections [8,9] due to the consumption of raw or undercook fish.Some medical properties of bitter cucumber (Mormordica charantia Linn.) include antiviral, antibacterial, anti-inflammatory, immunestimulant, insecticidal and anthelmintic properties. A bitter cucumber has been used to study about the anthelmintic activity on Ascaridia galli and Plasmodium berghei as antimalarial agent. A bitter cucumber (M. charantia Linn.) was shown that it was safe in experimental animals when ingested at low doses up to 2 months [10]. Previous study has confirmed that various preparations of papaya (Carica papaya Linn.) seeds can against helminthes effectively in vitro and in infected animals. Bioactive compounds responsible for the anthelmintic properties were ascribed to carpaine, carpasemine and benzyl isothiocyanate [11]. Most importantly, both papaya seeds and bitter cucumber fruits are cheap and easily available in tropical countries. However, there is no report that study regarding the effect of both extract of papaya seeds and bitter cucumber fruits on tegumetal surface of helminthes. Thus, this study is the first paper that study anthelmintic activity of both papaya seeds and bitter cucumber fruits on S. falcatus by observation under a Scanning Electron Microscope (SEM). Materials and Methods Preparation of the aqueous extract of papaya seeds and bitter cucumber fruitsThe aqueous extract of papaya seeds and bitter cucumber fruits were prepared according to the Thai Pharmacopoeia. Papaya seeds, 0.75 grams were dried and boiled in distilled water until the volume was 200 ml (3.75 mg/ml) then filtered through a sieved cloth to obtain a 100% stock solution. Twelve grams of bitter cucumber fruits was chopped and boiled in distilled water until the volume was 200 ml (60 mg/ml) and then filtered through a sieved cloth to get a 100% stock solution. The 100% stock solution was diluted in Tyrode's solution to give 12.5% and 50% concentration. Adult Stellantchasmus falcatus preparationThe chicks (Gallus gallus domesticus) were orally force fed with half-beaked fish (Dermogenus pusillus) infected with metacercariae of S. falcatus. Three days later, adult worms were collected from the small intestine of the chicks with Baremann's apparatus and washed with 0.85 % NaCl solution. The worms obtained were stored in Tyrode's solution. Experimental designThirty adult worms were individually incubated in Petri-dish containing 5 ml of Tyrode's solution, 12....

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Section: Discussionmentioning

confidence: 99%

In vitro Effects of Some Thai Anthelmintic Plants on Mortality and Change of Tegumental Surface of Stellantchasmus falcatus

Buddhachat¹,

Chantima²

2012

J Bacteriol Parasitol

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“…The tegument constitutes the parasitehost interface (Jones et al 2004;Van Hellemond et al 2006). Many proteins identified in the tegument of S. japonicum, S. mansoni, and F. gigantica were potentially molecules for vaccine development and for immunological diagnosis (Jeffs et al 1991;Waine et al 1994;Xavier et al 1998).…”

Section: Discussionmentioning

confidence: 99%

“…The outermost surface of intramammalian stages of platyhelminth parasite, the tegument, is generally viewed as the most susceptible target for vaccines and drugs because it is importance for host response and parasite survival (Van Hellemond et al 2006). In the present study, we described sequence analysis, expression, and characterization of one tegumental protein of C. sinensis, tegumental protein 31.8 kDa (CsTP31.8).…”

Section: Introductionmentioning

confidence: 99%

A novel tegumental protein 31.8 kDa of Clonorchis sinensis: sequence analysis, expression, and immunolocalization

Huang

Zhou

et al. 2007

Parasitol Res

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The complementary DNA (cDNA) plasmid libraries of adult worm, metacercariae and egg of Clonorchis sinensis (C. sinensis) were constructed for researches on genomics and proteomics of C. sinensis. The full-length cDNA sequence encoding tegumental protein 31.8 kDa (CsTP31.8) was identified from the adult cDNA library. The cDNA sequence has been submitted to the GeneBank Database with accession number ABK60086. This novel cDNA sequence contains 828 bp with a putative open reading frame of 275 amino acids. The deduced amino acid sequence shows identity to membrane-associate antigens or tegumental antigens of other species. There were conserved calcium-binding EF hand and dynein light chain type 1 in the sequence. CsTP31.8 transcripts were detected in cDNA libraries of adult worm and metacercariae but not in that of egg. Recombinant CsTP31.8 was expressed and purified from Escherichia coli BL21 (DE3). CsTP31.8 was immunolocalized at the tegument of adult C. sinensis by using antirecombinant CsTP31.8 sera.

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“…The tegumental outer surface of platyhelminth parasites is a unique double-membrane structure that is of crucial importance for modulation of the host response and parasite survival (Van Hellemond et al 2006). Tegumental proteins are structural proteins expressed in tegumental of different developmental stages.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and identification of a novel Clonorchis sinensis gene encoding a tegumental protein

Zhou

Huang

et al. 2007

Parasitol Res

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The tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. A cDNA encoding a novel tegumental protein 20.8 kDa (TP20.8) was found by large-scale sequencing of a Clonorchis sinensis cDNA library. This new cDNA was 755 bp long containing an open reading frame of 555 bp, which encoded a 20.8-kDa protein with an isoelectric point of 4.33. The deduced amino acid sequence exhibits 40 and 37% identity to Schistosoma japonicum sj20.8 and Schistosoma mansoni Sm 20.8, respectively. TP20.8 transcripts were detected in the adult worm and metacercariae cDNA libraries of C. sinensis but not in the egg. Recombinant C. sinensis TP20.8 protein was produced and purified from Escherichia coli BL21. Using specific anti-recombinant TP20.8 protein sera, the TP20.8 protein was immunohistochemically localized to the outer-surface membrane of C. sinensis. The specificity and sensitivity of the recombinant antigen for serologic diagnosis was assessed by enzyme-linked immunosorbent assay using serum from 100 patients with clonorchiasis, 20 patients with schistosomiasis, and 30 negative controls. The sensitivity was 68%, and the specificity was 84%. The antigen was less useful for the serodiagnosis of clonorchiasis with IgG.

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Functions of the tegument of schistosomes: Clues from the proteome and lipidome

Cited by 160 publications

References 70 publications

In vitro Effects of Some Thai Anthelmintic Plants on Mortality and Change of Tegumental Surface of Stellantchasmus falcatus

In vitro Effects of Some Thai Anthelmintic Plants on Mortality and Change of Tegumental Surface of Stellantchasmus falcatus

A novel tegumental protein 31.8 kDa of Clonorchis sinensis: sequence analysis, expression, and immunolocalization

Molecular cloning and identification of a novel Clonorchis sinensis gene encoding a tegumental protein

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