Functions of miR-1 and miR-133a during the postnatal development of masseter and gastrocnemius muscles

Abstract

The present study investigated the function of miR-1 and miR-133a during the postnatal development of mouse skeletal muscles. The amounts of miR-1 and miR-133a were measured in mouse masseter and gastrocnemius muscles between 1 and 12 weeks after birth with real-time polymerase chain reaction and those of HDACs, MEF2, MyoD family, MCK, SRF, and Cyclin D1 were measured at 2 and 12 weeks with Western blotting. In both the masseter and gastrocnemius muscles, the amount of miR-1 increased between 1 and 12 weeks, whereas the amount of HADC4 decreased between 2 and 12 weeks. In the masseter muscle, those of MEF2, MyoD, Myogenin, and MCK increased between 2 and 12 weeks, whereas, in the gastrocnemius muscle, only those of MRF4 and MCK increased. The extent of these changes in the masseter muscle was greater than that in the gastrocnemius muscle. The amounts of miR-133a, SRF, and Cyclin D1 did not change significantly in the masseter muscle between 1 and 12 weeks after birth. By contrast, in the gastrocnemius muscle, the amounts of miR-133a and Cyclin D1 increased, whereas that of SRF decreased. Our findings suggest that the regulatory pathway of miR-1 via HDAC4 and MEF2 plays a more prominent role during postnatal development in the masseter muscle than in the gastrocnemius muscle, whereas that of miR-133a via SRF plays a more prominent role in the gastrocnemius muscle than in the masseter muscle.

Effects of aerobic and inspiratory training on skeletal muscle microRNA‐1 and downstream‐associated pathways in patients with heart failure

“…Increased levels of microRNA‐1 can be also involved in postnatal myoblast differentiation by suppressing HDAC4, a predicted target gene of this myomiR . HDACs are well known for regulating muscle proliferation, differentiation, and growth through the regulation of histone acetylation.…”

“…In particular, class IIa HDACs (HDACs 4, 5, 7, and 9) plays an important role in the maintenance of muscle mass and protein degradation during muscle wasting . The reduced levels of HDAC4 promote myoblast differentiation, while increased levels of HDAC4 enhances skeletal muscle atrophy . Chen et al .…”

“…Skeletal muscle‐enriched microRNAs (myomiRs) play an important role in the regulation of muscle development, growth, regeneration, and metabolism . The microRNA‐1 and microRNA‐133a can regulate myogenesis by suppressing the predicted target gene histone deacetylase 4 (HDAC4), which upregulates myogenic markers as myocyte enhancer factor 2C (MEF2c), myogenic differentiation factor D (MyoD), and follistatin expression . Whether alterations in microRNA‐1 and microRNA‐133a and downstream‐associated pathways contribute to skeletal myopathy in patients with chronic HFrEF is unknown.…”

miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis

“…Thus far, many miRNAs have been shown to regulate the proliferation and differentiation of myoblasts during muscle myogenesis. Previous studies have reported that several miRNAs, including miR-1 13 , 14 , miR-133 15 , 16 and miR-206 17 , 18 , are muscle-specific miRNAs because their key regulators are involved in muscle myogenesis in humans, mice and cows. Some non-specific miRNAs in muscle, such as miR-24 19 , miR-26a 20 , miR-29 21 , miR-125b 22 , miR-181 23 , miR-214 24 and miR-491 25 , are also essential for the regulation of muscle myogenesis.…”

miR-378a-3p promotes differentiation and inhibits proliferation of myoblasts by targeting HDAC4 in skeletal muscle development

“…These miRNAs also play roles in muscle development in mammals. Previous studies reported that several miRNAs, such as miR-1, 47 miR-133, 31,45,49 and miR-206, 3,19,42 are musclespecific miRNAs because they are key regulators in muscle development in human, pigs, and rats. Others miRNAs may also contribute to muscle development.…”