Functions and mechanisms of the CBL–CIPK signaling system in plant response to abiotic stress

Li, Ruifen; Zhang, Junwen; Wei, Jianhua; Wang, Hongzhi; Wang, Yanzhen; Ma, Ruijuan

doi:10.1016/j.pnsc.2008.06.030

Cited by 63 publications

(51 citation statements)

References 50 publications

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“…Calcium-dependent protein kinases (CDPKs) can bind and sense calcium ions directly to induce the downstream signaling in response to different stresses [11]. The CBL-CIPK signaling system, a newly emerging plant-specific and Ca 2+ -dependent network, mediates abiotic stress tolerance, for substances such as salt [60]. In this study, MAPK and CDPK signaling pathways were up-regulated under salt stress, which were similar to the result of Zhang et al [32].…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptional Profiling of Two Contrasting Barley Genotypes under Salinity Stress during the Seedling Stage

Gao

Guo³

et al. 2013

International Journal of Genomics

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Salinity is one of the major abiotic stresses that affect crop productivity. Identification of the potential novel genes responsible for salt tolerance in barley will contribute to understanding the molecular mechanism of barley responses to salt stress. We compared changes in transcriptome between Hua 11 (a salt-tolerant genotype) and Hua 30 (a salt sensitive genotype) in response to salt stress at the seedling stage using barley cDNA microarrays. In total, 557 and 247 salt-responsive genes were expressed exclusively in the shoot and root tissue of the salt-tolerant genotype, respectively. Among these genes, a number of signal-related genes, transcription factors and compatible solutes were identified and some of these genes were carefully discussed. Notably, a LysM RLK was firstly found involved in salt stress response. Moreover, key enzymes in the pathways of jasmonic acid biosynthesis, lipid metabolism and indole-3-acetic acid homeostasis were specifically affected by salt stress in salt tolerance genotype. These salt-responsive genes and biochemical pathways identified in this study could provide further information for understanding the mechanisms of salt tolerance in barley.

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Section: Discussionmentioning

confidence: 99%

Comparative Transcriptional Profiling of Two Contrasting Barley Genotypes under Salinity Stress during the Seedling Stage

Gao

Guo³

et al. 2013

International Journal of Genomics

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“…Similarly, in maize ZmCIPKs, microarray data and reverse transcription polymerase chain reaction (RT-PCR) assay results showed that genes were transcriptionally responsive to abiotic stresses and that 19, 20, 24, and 31 ZmCIPK genes were upregulated by salt, drought, heat, and cold stresses, respectively (Chen et al 2011b). Other studies have shown that the CBL-CIPK system plays key roles in plant responses to abiotic stresses (Albrecht et al 2003;Gong et al 2002a, b;Li et al 2006Li et al , 2009Mahajan et al 2008;Nozawa et al 2001;Pandey et al 2004;Vranova et al 2002;Xu et al 2006). However, the function of additional CIPK proteins remains to be revealed.…”

Section: Introductionmentioning

confidence: 97%

Overexpression of BrCIPK1 Gene Enhances Abiotic Stress Tolerance by Increasing Proline Biosynthesis in Rice

et al. 2015

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The calcineurin B-like protein (CBL)-CBLinteracting protein kinase (CIPK) pathway is emerging as a major signaling pathway in plants. To understand the function of CIPK, the gene named BrCIPK1 from Brassica rapa were introduced into rice. Characterization of BrCIPK1 gene showed a 1982 bp, containing 1509 bp coding region and 502 amino acids. Green fluorescent protein (GFP)-tagged BrCIPK1 was observed exclusively in the cytoplasmic and peripheral regions in the plant cell. Gene expression showed that its messenger RNA (mRNA) transcription in B. rapa was differentially accumulated in the presence of cold, salinity, and drought, indicating its biological roles in multiple stress response pathways in plants. Furthermore, Ubi-1::BrCIPK1 rice lines showed significantly higher biomass, water content, and proline and free sugar content relative to those in the wild-type Gopum. The BrCIPK1 interacted with rice calcineurin B-like protein 1 and 5 (OsCBL1, OsCBL5), suggesting that it is activated by Ca 2+ -bound CBLs in the cytosol by calcium spiking and regulates its downstream target proteins in these regions to increase abiotic stress tolerance. The results imply that BrCIPK1 gene may be involved in stress adaptations through the activation of pyrroline-5-carboxylate synthase in the proline biosynthetic pathway. In this paper, a hypothetical mechanism of elevated tolerance to cold, drought, and salinity is presented.

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“…Most stresses elicit temporal and spatial changes in cytosolic free Ca 2+ concentrations (Sanders et al, 2002). signals are decoded by Ca 2+ sensors, and transduced through interactions with their protein kinases to regulate the expression of stress-responsive genes (Li et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum

et al. 2014

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ABSTRACT. CBL-interacting protein kinases (CIPKs) mediate many plant responses to abiotic stress. However, their functions are poorly understood in halophytes. In this study, we isolated a CIPK gene, NtCIPK2, from the halophyte Nitraria tangutorum. By sequence alignment and the construction of a phylogenetic tree, we found that NtCIPK2 is similar to CIPK2 proteins from other plants, and contains conserved domains and motifs. The promoter of NtCIPK2 harbors many cis-acting elements that might be recognized and bound by transcription factors that are related to hormones and stress responses. NtCIPK2 was ubiquitously and robustly expressed in all tested organs, and was induced by salinity, drought, heat, and cold stress. The overexpression of NtCIPK2 in Escherichia coli caused better growth against high salinity, alkalinity, and osmotic conditions, dehydration, and extreme temperatures (i.e., heat and cold) compared to the control. 4717©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 4716-4728 (2014) Cloning and characterization of the NtCIPK2 gene Thus, NtCIPK2 is a candidate gene that might improve the stress tolerance of crops and herbs through genetic manipulation.

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Functions and mechanisms of the CBL–CIPK signaling system in plant response to abiotic stress

Cited by 63 publications

References 50 publications

Comparative Transcriptional Profiling of Two Contrasting Barley Genotypes under Salinity Stress during the Seedling Stage

Comparative Transcriptional Profiling of Two Contrasting Barley Genotypes under Salinity Stress during the Seedling Stage

Overexpression of BrCIPK1 Gene Enhances Abiotic Stress Tolerance by Increasing Proline Biosynthesis in Rice

Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum

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