Functions and mechanisms of the Ubc13-UEV complex and lysine 63-linked polyubiquitination in plants

Yang, Kun; Xiao, Wei

doi:10.1093/jxb/erac239

Cited by 4 publications

(2 citation statements)

References 171 publications

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“…Subsequent studies reveal that AtUBC13 s function in apical dominance [ 23 ], DDR [ 22 ], iron metabolism [ 24 ], auxin signaling [ 25 ], low temperature stress response [ 26 ] and plant immunity [ 26 , 27 ]. In addition, systematic studies in Arabidopsis revealed additional target proteins modified by K63-linked polyubiquitination [ 28 , 29 ], indicating that plant Ubc13s play multiple roles in plant development and stress responses.…”

Section: Introductionmentioning

confidence: 99%

Systematic characterization of Brassica napus UBC13 genes involved in DNA-damage response and K63-linked polyubiquitination

et al. 2023

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Background Ubc13 is the only known ubiquitin conjugating enzyme (Ubc/E2) dedicated to promoting Lys (K)63-linked polyubiquitination, and this process requires a Ubc/E2 variant (UEV). Unlike conventional K48-linked polyubiquitination that targets proteins for degradation, K63-linked polyubiquitination, which is involved in several cellular processes, does not target proteins for degradation but alter their activities. Results In this study we report the identification and functional characterization of 12 Brassica napus UBC13 genes. All the cloned UBC13 gene products were able to physically interact with AtUev1D, an Arabidopsis UEV, to form stable complexes that are capable of catalyzing K63-linked polyubiquitination in vitro. Furthermore, BnUBC13 genes functionally complemented the yeast ubc13 null mutant defects in spontaneous mutagenesis and DNA-damage responses, suggesting that BnUBC13s can replace yeast UBC13 in mediating K63-linked polyubiquitination and error-free DNA-damage tolerance. Conclusion Collectively, this study provides convincing data to support notions that B. napus Ubc13s promote K63-linked polyubiquitination and are probably required for abiotic stress response. Since plant Ubc13-UEV are also implicated in other developmental and stress responses, this systematic study sets a milestone in exploring roles of K63-linked polyubiquitination in this agriculturally important crop.

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Section: Introductionmentioning

confidence: 99%

Systematic characterization of Brassica napus UBC13 genes involved in DNA-damage response and K63-linked polyubiquitination

et al. 2023

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“… 14 Single ubiquitin molecules have seven surface Lys residues (K6, K11, K27, K29, K33, K48, and K63), and ubiquitin molecules can be sequentially added to one of their own Lys residues, known as polyubiquitination. 15 Various types of chains play different roles in different cellular processes, the most widely studied of which are K48, K63, and linear ubiquitin chains. As TRIM55 is an E3 ubiquitin ligase, the specific mechanism of ubiquitination is unknown.…”

Section: Introductionmentioning

confidence: 99%

TRIM55 Promotes Proliferation of Hepatocellular Carcinoma Through Stabilizing TRIP6 to Activate Wnt/β-Catenin Signaling

Yuan¹,

Cai²,

Rao³

et al. 2023

JHC

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Purpose Tripartite motif containing 55 (TRIM55) is a member of the TRIM family and functions as an E3 ubiquitin ligase. It acts as a cancer promoter or suppressor in the malignant processes of multiple cancers. However, its proliferative function in hepatocellular carcinoma (HCC) has been poorly studied, and its underlying molecular mechanism remains unclear. In the present study, we investigated the role of TRIM55 in HCC and its mechanism of promoting HCC proliferation. Materials and Methods Protein expression levels of TRIM55 were measured in paired HCC and normal tissue samples using immunohistochemical (IHC) staining. The correlation between TRIM55 and clinical features was evaluated by statistical analysis. At the same time, overexpression and knockdown experiments, cycloheximide (CHX) interference experiments, ubiquitination, co-immunoprecipitation and immunofluorescence staining experiments, as well as animal experiments were used to evaluate the potential mechanism that TRIM55 promotes proliferation of hepatocellular carcinoma in vitro and in vivo. Results TRIM55 expression in HCC specimens was higher compared with the corresponding non-tumor tissues. The overall survival and disease-free survival time of patients with high TRIM55 expression were shorter than those with low expression of TRIM55. Functionally, TRIM55 promoted the proliferation of HCC cells and accelerated the growth of HCC xenografts. Mechanistically, TRIM55 interacted with thyroid receptor interacting protein 6 (TRIP6) and regulate its stability by influencing the ubiquitination process, thereby affecting the Wnt signaling pathway. Conclusion Our results indicate that TRIM55 promotes HCC proliferation by activating Wnt signaling pathways by stabilizing TRIP6. Therefore, targeting TRIM55 may be an effective therapeutic strategy to inhibit HCC growth.

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Genetic analysis of DNA-damage tolerance pathways in Arabidopsis

et al. 2022

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Functions and mechanisms of the Ubc13-UEV complex and lysine 63-linked polyubiquitination in plants

Cited by 4 publications

References 171 publications

Systematic characterization of Brassica napus UBC13 genes involved in DNA-damage response and K63-linked polyubiquitination

Systematic characterization of Brassica napus UBC13 genes involved in DNA-damage response and K63-linked polyubiquitination

TRIM55 Promotes Proliferation of Hepatocellular Carcinoma Through Stabilizing TRIP6 to Activate Wnt/β-Catenin Signaling

Genetic analysis of DNA-damage tolerance pathways in Arabidopsis

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