Functioning of the mercury resistance operon at extremely high Hg(II) loads in a chemostat: A proteome analysis

Leonhäuser, J.; Wang, Wei; Deckwer, Wolf‐Dieter; Wagner‐Döbler, Irene

doi:10.1016/j.jbiotec.2007.08.018

Cited by 10 publications

(4 citation statements)

References 26 publications

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“…However, a cation efflux transporter was also strongly upregulated and an outer membrane porin strongly downregulated, which probably served to further reduce Hg 2+ toxicity, thereby complementing the effects of the mercury‐resistance genes. No indication of any higher demand for energy was detected, perhaps due to the highly efficient reduction of Hg 2+ to Hg 0 .…”

Section: Response To Environmental Stressmentioning

confidence: 95%

The contribution of proteomics to the unveiling of the survival strategies used by Pseudomonas putida in changing and hostile environments

Moreno

Rojo

2013

Proteomics

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Pseudomonas putida is a ubiquitous, metabolically very versatile, Gram-negative bacterium adapted to habitats as diverse as soil, water and the rhizosphere. Most strains are nonpathogenic, many are used as experimental models, and many others have biotechnological applications in the areas of agriculture, bioremediation, biocatalysis, and the production of bioplastics. This review summarizes the contribution of proteomic technologies to our understanding of how P. putida responds to different carbon sources, how it adapts to living at suboptimal temperatures or attached to surfaces, and how it responds to the presence of toxic compounds such as aromatic molecules and heavy metals. The examples described illustrate the value of proteomics in furthering our knowledge of the physiology and behavior of bacteria, knowledge that is important for understanding how they behave in their natural habitats and for optimizing their behavior in biotechnological applications.

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Section: Response To Environmental Stressmentioning

confidence: 95%

The contribution of proteomics to the unveiling of the survival strategies used by Pseudomonas putida in changing and hostile environments

Moreno

Rojo

2013

Proteomics

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“…A merA PCR product was obtained in 22 of the initially isolated strains, including all highly and moderately resistant strains except TR63 (Table 1). Interestingly, a merA gene was also detected in three strains classified as sensitive, suggesting either that other mer genes in the operon were missing or that the presence of a mer operon does not guarantee high levels of Hg resistance, and therefore, the involvement of additional factors is required in the resistance mechanism (Leonhäuser et al 2007). The importance of such additional resistance mechanisms against Hg toxicity in the absence of a merA gene is specially suggested in Bacillus toyonensis strain TR63, which shows resistance to high levels of HgCl 2 despite not showing a merA copy in its genome.…”

Section: Presence and Diversity Of Hg Resistance Genesmentioning

confidence: 99%

Highly mercury-resistant strains from different Colombian Amazon ecosystems affected by artisanal gold mining activities

Cardona

Escobar

Acosta-González

et al. 2022

Appl Microbiol Biotechnol

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Two sites of the Colombian Amazon region with different levels of human intervention and mercury pollution were selected for the collection of samples of river and lake water, sediments, and associated forest soils. The Tarapacá region, affected mainly by barrage mining, showed low mercury concentrations, whilst in the Taraira region, affected by underground mining, there were several points with high mercury pollution levels. A collection of 72 bacterial and 10 yeast strains with different levels of mercury resistance was isolated and characterized. Most of the highly resistant bacterial strains (MIC > 40 mg L−1 HgCl2) were isolated from soil and sediment samples and belonged to either Pseudomonas (60%) or Bacillus (20%). Most of highly resistant bacterial strains were positive for the presence of the merA gene, suggesting an active mercury resistance mechanism. This was confirmed in the two most resistant strains, Pseudomonas sp. TP30 and Burkholderia contaminans TR100 (MIC = 64 and 71 mg L−1 HgCl2, respectively), which in the presence of increasing mercury concentrations expressed the merA gene at increasing levels, concomitant with a significant mercury reduction activity. Analysis of the MerA sequences present in the different isolates suggested a high gene conservation within the taxonomic groups but also several horizontal gene transfer events between taxonomically distant genera. We also observed a positive correspondence between the presence of the merA gene and the number of antibiotics to which the strains were resistant to. The most resistant strains are good candidates for future applications in the bioremediation of mercury-contaminated sites in the Amazon.Key points• Amazon sediments affected by underground gold mining have higher Hg levels.• Highly Hg-resistant isolates belonged to Pseudomonas and Bacillus genera.• TR100 and TP30 strains showed remediation potential to be used in the Amazon region. Graphical abstract

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“…P. pseudoalcaligenes S1 was preliminarily investigated by us ( 4 ) for its bioaccumulation and attractive mercury resistance which has the highest value reported so far ( 5 , 6 ). It has generally been accepted that the mercury resistance of microorganisms depends on the intracellular expression of MerA, a mercuric reductase conferring cells to reduce Hg 2+ to Hg 0 ( 7 ). In this case, microbial cells will not exhibit high Hg 2+ accumulation capacity, because Hg 0 is subject to volatilization into the air.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of a Marine Bacterium, Pseudomonas pseudoalcaligenes Strain S1, with High Mercury Resistance and Bioaccumulation Capacity

Liu

Bian²,

Huang

et al. 2016

Genome Announc

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Functioning of the mercury resistance operon at extremely high Hg(II) loads in a chemostat: A proteome analysis

Cited by 10 publications

References 26 publications

The contribution of proteomics to the unveiling of the survival strategies used by Pseudomonas putida in changing and hostile environments

The contribution of proteomics to the unveiling of the survival strategies used by Pseudomonas putida in changing and hostile environments

Highly mercury-resistant strains from different Colombian Amazon ecosystems affected by artisanal gold mining activities

Complete Genome Sequence of a Marine Bacterium, Pseudomonas pseudoalcaligenes Strain S1, with High Mercury Resistance and Bioaccumulation Capacity

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