Functionalizing Framework Nucleic‐Acid‐Based Nanostructures for Biomedical Application

Zhang, Tao; Tian, Taoran; Lin, Yunfeng

doi:10.1002/adma.202107820

Cited by 160 publications

(133 citation statements)

References 187 publications

(249 reference statements)

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“…In recent years, there has been rapid progress in the development of DNA nanomaterials for biomedical applications due to their excellent editability. 9 Among these DNA nanomaterials, tetrahedral framework nucleic acids (tFNAs), which can be easily synthesized and edited, have become a major research focus owing to their 3D polyhedral structure and broad biological features. 10 Extensive studies have revealed that tFNAs alone can regulate several cellular processes, such as migration, proliferation, differentiation, and autophagy.…”

Section: Introductionmentioning

confidence: 99%

Treatment effect of DNA framework nucleic acids on diffuse microvascular endothelial cell injury after subarachnoid hemorrhage

Chen

Wen

et al. 2022

Cell Proliferation

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Objectives The purpose of this study was to investigate the treatment effect and molecular mechanism of tetrahedral framework nucleic acids (tFNAs), novel self‐assembled nucleic acid nanomaterials, in diffuse BMEC injury after SAH. Materials and Methods tFNAs were synthesized from four ssDNAs. The effects of tFNAs on SAH‐induced diffuse BMEC injury were explored by a cytotoxicity model induced by hemin, a breakdown product of hemoglobin, in vitro and a mouse model of SAH via internal carotid artery puncture in vivo. Cell viability assays, wound healing assays, transwell assays, and tube formation assays were performed to explore cellular function like angiogenesis. Results In vitro cellular function assays demonstrated that tFNAs could alleviate hemin‐induced injury, promote angiogenesis, and inhibit apoptosis in hemin cytotoxicity model. In vivo study using H&E and TEM results jointly indicated that the tFNAs attenuate the damage caused by SAH in situ, showing restored number of BMECs in the endothelium layer and more tight intercellular connectivity. Histological examination of SAH model animals confirmed the results of the in vitro study, as tFNAs exhibited treatment effects against diffuse BMEC injury in the cerebral microvascular bed. Conclusions Our study suggests the potential of tFNAs in ameliorating diffuse injury to BMECs after SAH, which laid theoretical foundation for the further study and use of these nucleic acid nanomaterials for tissue engineering vascularization.

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Section: Introductionmentioning

confidence: 99%

Treatment effect of DNA framework nucleic acids on diffuse microvascular endothelial cell injury after subarachnoid hemorrhage

Chen

Wen

et al. 2022

Cell Proliferation

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“…Histone deacetylase 5 (HDAC5) in bone marrow mesenchymal stem cells play an important role in controlling bone remodeling. Recent studies used the tetrahedral nucleic acids framework (tFNAs) to transport miR-2861, which could inhibit HDAC5 expression, promoting osteogenic differentiation (Li et al, 2021a;Zhang et al, 2021). Sclerostin, an inhibitor of the Wnt/β-catenin signaling pathway that regulates bone growth, has emerged as an attractive therapeutic target for the treatment of osteoporosis (Suen and Qin, 2016).…”

Section: Introductionmentioning

confidence: 99%

Cell-Free Fat Extract Prevents Tail Suspension–Induced Bone Loss by Inhibiting Osteocyte Apoptosis

Cui

et al. 2022

Front. Bioeng. Biotechnol.

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Introduction: As the space field has developed and our population ages, people engaged in space travel and those on prolonged bed rest are at increasing risk for bone loss and fractures. Disuse osteoporosis occurs frequently in these instances, for which the currently available anti-osteoporosis agents are far from satisfactory and have undesirable side effects. CEFFE is a cell-free fraction isolated from nanofat that is enriched with a variety of growth factors, and we aim to investigate its potential therapeutic effects on disuse osteoporosis.Methods: A tail suspension–induced osteoporosis model was applied in this study. Three weeks after tail suspension, CEFFE was intraperitoneally injected, and PBS was used as a control. The trabecular and cortical bone microstructures of the tibia in each group were assessed by μCT after 4 weeks of administration. Osteocyte lacunar-canalicularity was observed by HE and silver staining. In vitro, MLO-Y4 cell apoptosis was induced by reactive oxygen species (ROSUP). TUNEL staining and flow cytometry were used to detect apoptosis. CCK-8 was used to detect cell proliferation, and Western blotting was used to detect MAPK signaling pathway changes.Results: CEFFE increased the bone volume (BV/TV) and trabecular number (Tb.N) of the trabecular bone and increased the thickness of the cortical bone. HE and silver staining results showed that CEFFE reduced the number of empty lacunae and improved the lacuna-canalicular structure. CEFFE promoted osteocyte proliferative capacity in a dose-dependent manner. CEFFE protected MLO-Y4 from apoptosis by activating the serine/threonine-selective protein kinase (ERK) signaling pathways.Conclusion: CEFFE attenuated immobilization-induced bone loss by decreasing osteocyte apoptosis. CEFFE increased the survival of osteocytes and inhibited osteocyte apoptosis by activating the ERK signaling pathway in vitro.

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“…For the foreseeable future, the application of multiomics technologies, such as transcriptomics, proteomics, and metabonomics, can systematically study the interaction between intracellular signaling pathways and metabolic pathways in osteogenesis from different dimensions. The development of corresponding targeted drugs against targets of energy metabolism during osteogenesis to promote bone regeneration is also worth investigating, such as the application of tFNA, and there are current studies that target mir-2861 to BMSCs to promote osteogenesis by inhibiting the expression of histone deacetylase 5 (hdac5) to maintain histone acetylation (Li et al, 2021;Zhang et al, 2021). The in-depth exploration of the interaction between intracellular energy metabolism and signaling pathways during osteogenesis will promote the development of basic biology and provide new therapeutic targets and strategies for osteoporosis.…”

Section: Discussionmentioning

confidence: 99%

The Interaction Between Intracellular Energy Metabolism and Signaling Pathways During Osteogenesis

Xiao

Wang

et al. 2022

Front. Mol. Biosci.

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Osteoblasts primarily mediate bone formation, maintain bone structure, and regulate bone mineralization, which plays an important role in bone remodeling. In the past decades, the roles of cytokines, signaling proteins, and transcription factors in osteoblasts have been widely studied. However, whether the energy metabolism of cells can be regulated by these factors to affect the differentiation and functioning of osteoblasts has not been explored in depth. In addition, the signaling and energy metabolism pathways are not independent but closely connected. Although energy metabolism is mediated by signaling pathways, some intermediates of energy metabolism can participate in protein post-translational modification. The content of intermediates, such as acetyl coenzyme A (acetyl CoA) and uridine diphosphate N-acetylglucosamine (UDP-N-acetylglucosamine), determines the degree of acetylation and glycosylation in terms of the availability of energy-producing substrates. The utilization of intracellular metabolic resources and cell survival, proliferation, and differentiation are all related to the integration of metabolic and signaling pathways. In this paper, the interaction between the energy metabolism pathway and osteogenic signaling pathway in osteoblasts and bone marrow mesenchymal stem cells (BMSCs) will be discussed.

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Functionalizing Framework Nucleic‐Acid‐Based Nanostructures for Biomedical Application

Cited by 160 publications

References 187 publications

Treatment effect of DNA framework nucleic acids on diffuse microvascular endothelial cell injury after subarachnoid hemorrhage

Treatment effect of DNA framework nucleic acids on diffuse microvascular endothelial cell injury after subarachnoid hemorrhage

Cell-Free Fat Extract Prevents Tail Suspension–Induced Bone Loss by Inhibiting Osteocyte Apoptosis

The Interaction Between Intracellular Energy Metabolism and Signaling Pathways During Osteogenesis

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