Functionalized tricalcium phosphate and poly(3-hydroxyoctanoate) derived composite scaffolds as platforms for the controlled release of diclofenac

Physicochemical and Biological Characterisation of Diclofenac Oligomeric Poly(3-hydroxyoctanoate) Hybrids as β-TCP Ceramics Modifiers for Bone Tissue Regeneration

“…From the analysis, it can be concluded that the amount of active substance released during the 24 h of incubation does not cause a cytotoxic effect when in contact with the MC3T3-E1 cell line. In the case of β-TCP/P(3HO) composites containing physically bounded DIC, analogous phenomenon was reported (99.20 ± 5.61%) [41]. Moreover, a similar effect was observed by Sidney et al after a 14-day incubation of the mouse preosteoblast cells in the culture medium and further addition of 1 mg of sodium DIC per scaffold (8fold lower than that used in our study) [5].…”

“…Additionally, the release of polymer decomposition products—( R )-3-hydroxyacids—stimulates cell viability [ 58 ]. The lack of cytotoxicity of materials made of β-TCP is confirmed by the results obtained by Skibiński et al Indirect cytotoxicity tests of β-TCP sponges performed in the MC3T3-E1 model showed high cell viability (117.11 ± 5.59%) [ 41 ]. In the case of composites infiltrated with blends containing different amounts of Fin-Dic-oliP(3HO), no cytotoxic effects with respect to the negative control are observed.…”

“…To obtain polymeric coatings, three blends of P(3HO) with drug-modified oligomers were prepared: two (2Dic-oliP(3HO)), one (1Dic-oliP(3HO)) and a half (0.5Dic-oliP(3HO)) of a therapeutic DIC dose [ 40 ] (1 dose = 0.19 g DIC per 1 g of polymer) ( Table 1 ). In this order, an appropriate amount of P(3HO) was dissolved in 50 mL of dichloromethane at 60 °C, which followed the addition of an appropriate amount of oligo-DIC [ 41 ]. The constituents were mixed for 3 h at an ambient temperature after the solvent was evaporated.…”

“…The indirect cytotoxicity studies were carried out to evaluate the presence of toxic compounds in the extracts obtained from the scaffolds, following the ISO 10993-5, using a protocol described by Skibiński et al [ 41 ].…”

Development of highly porous calcium phosphate bone cements applying nonionic surface active agents

“…Different techniques are used to obtain highly porous CaPs scaffolds. The method of replication of porous organic matrix [4][5][6][7] is a well-known fabrication technique for the ceramic bone scaffolds. During this process, a polymer sponge (usually polyurethane) is soaked in a ceramic slurry, dried and nally submitted to thermal treatment and sintering.…”