Functionalized TiO2 Nanotube Platform for Gliadin Electroanalysis

Abstract

The present paper presents a gliadin detection method. This method is based on a modified Ti electrode. Modification was performed by a simple and cheap anodization. Then, a layer of graphene oxide was added, and gliadin antibody was fixed on the electrode surface. Using this complex system, electrochemical impedance spectroscopy was used for gliadin detection. Solutions with known gliadin (a fraction from gluten) content were used for analysis. Impedance measured at a certain frequency and coating resistance were analyzed. Better results (good linearity and lower detection limit) were obtained by plotting impedance at a certain frequency versus gliadin concentration. Coating resistance was proved to be in linear dependency with gliadin concentration only at lower concentrations. This system based on titanium nanostructured electrode has the potential to be used for gluten contamination detection from foods.

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

“…Pirvu and co-workers [ 78 ] developed a label-free immunosensor based on TiO 2 nanotubes (TiO 2 NTs), GO, and gliadin antibodies. It is well known that TiO 2 NTs were used in sensor applications because they can be easily prepared with high reproducibility and low cost, they have a large surface area, and they are non-toxic, hydrophilic, and biocompatible [ 78 , 79 ]. These nanostructures also have other important characteristics: they have antibacterial activities and confer UV protection [ 80 , 81 ].…”