Functionalized silica nanoplatform as a bimodal contrast agent for MRI and optical imaging

Abstract

The preparation of an efficient bimodal single probe for magnetic resonance (MRI) and optical imaging (OI) is reported. Paramagnetic properties have been obtained by the non-covalent encapsulation of the clinically...

Doubly Strapped Zwitterionic NIR‐I and NIR‐II Heptamethine Cyanine Dyes for Bioconjugation and Fluorescence Imaging

“…One particularly promising conjugate, with an appended cyclic‐RGD peptide motif that targets cancerous tissue, has progressed to clinical trials [20, 21] . There are also a growing number of reports that use ZW800‐1 or close structural analogues to label macromolecules for in vivo imaging and histology applications, [17, 22–24] or biomaterials such as cell surfaces, hydrogels, nanoparticles, 3D printed biocomposites, or extracellular matrix biopolymers for tissue engineering applications [25–32] . A necessary dye performance property for most of these applications is long‐term chemical stability of the fluorescent bioconjugate and this requirement highlights a potential chemical reactivity problem with ZW800‐1 ; namely, linker cleavage due to nucleophilic substitution of the dye's 4′‐phenoxy group by biological nucleophiles (illustrated by the pink arrow in Scheme 1).…”

Preparation and Application of a Bioorganic Nanoparticle-Enhanced PDL1-Targeted Small-Molecule Probe

“…Several studies have shown that nanoimaging probes can improve tumor targeting and the residence time of small molecules, thereby expanding their potential uses in imaging and therapeutic efficacy. 23,24 Melanin is a naturally occurring substance. The surface of melanin nanoparticles (MNPs) contains many functional groups that can be modified, allowing them to be labeled directly with various metal ions and metallic isotopes without coupling agents via electrophilic substitution of radioiodine or exploiting the metal chelating function.…”

“…Nanoparticles encompass a rich array of types and functions and can greatly improve drug delivery efficiency and retention times via passive targeting. Several studies have shown that nanoimaging probes can improve tumor targeting and the residence time of small molecules, thereby expanding their potential uses in imaging and therapeutic efficacy. , Melanin is a naturally occurring substance. The surface of melanin nanoparticles (MNPs) contains many functional groups that can be modified, allowing them to be labeled directly with various metal ions and metallic isotopes without coupling agents via electrophilic substitution of radioiodine or exploiting the metal chelating function. , Melanin nanoparticles are safe, biocompatible, and can be generated by the synthesis of natural melanin or dopamine polymerization. − In our previous research, we synthesized second-generation melanin nanoparticles (MNPs) and developed a safe, biocompatible, and stable multimodal imaging and therapy probe targeting PSMA, SSTR2, or HER2 in vivo. − We hypothesized that a combination of WL12 and melanin nanoparticles could be used to improve the specificity and functionality of PDL1 PET imaging.…”

Doubly Strapped Zwitterionic NIR‐I and NIR‐II Heptamethine Cyanine Dyes for Bioconjugation and Fluorescence Imaging

“…[20,21] There are also a growing number of reports that use ZW800-1 or close structural analogues to label macromolecules for in vivo imaging and histology applications, [17,[22][23][24] or biomaterials such as cell surfaces, hydrogels, nanoparticles, 3D printed biocomposites, or extracellular matrix biopolymers for tissue engineering applications. [25][26][27][28][29][30][31][32] A necessary dye performance property for most of these applications is long-term chemical stability of the fluorescent bioconjugate and this requirement highlights a potential chemical reactivity problem with ZW800-1; namely, linker cleavage due to nucleophilic substitution of the dye's 4'-phenoxy group by biological nucleophiles (illustrated by the pink arrow in Scheme 1). [33] Studies that have incubated samples of antibodies or nanoparticles labeled with ZW800-1 in serum for 24 hours have observed substantial loss of fluorescent signal.…”

“…One particularly promising conjugate, with an appended cyclic‐RGD peptide motif that targets cancerous tissue, has progressed to clinical trials [20, 21] . There are also a growing number of reports that use ZW800‐1 or close structural analogues to label macromolecules for in vivo imaging and histology applications, [17, 22–24] or biomaterials such as cell surfaces, hydrogels, nanoparticles, 3D printed biocomposites, or extracellular matrix biopolymers for tissue engineering applications [25–32] . A necessary dye performance property for most of these applications is long‐term chemical stability of the fluorescent bioconjugate and this requirement highlights a potential chemical reactivity problem with ZW800‐1 ; namely, linker cleavage due to nucleophilic substitution of the dye's 4′‐phenoxy group by biological nucleophiles (illustrated by the pink arrow in Scheme 1).…”