“…Thus, different cationic lipids such as lipophosphonates [ 15 , 16 , 17 ] and lipophosphoramidates [ 12 , 13 , 18 , 19 , 20 , 21 ] have been produced, and their high ability to transfect cells in culture and lungs mice were demonstrated [ 13 , 22 , 23 , 24 , 25 , 26 , 27 ]. However, the plasmid type [ 27 , 28 , 29 ], the vector [ 18 , 26 , 29 , 30 , 31 , 32 ] as well as the nucleic acids (NA) or synthetic carrier’s labeling techniques [ 8 , 30 , 33 , 34 , 35 ], impact greatly the distribution in tissues as well as the transgene expression. Labeling liposomes with a fluorescent probe appears as a relevant procedure to establish biodistribution profiles of lipoplexes and their pharmacokinetics, irrespective of administration route [ 26 , 27 , 31 , 32 , 33 , 34 , 36 , 37 , 38 ].…”