Functionalized nanoparticles enable tracking the rapid entry and release of doxorubicin in human pancreatic cancer cells

Arachchige, Maheshika Palihawadana; Laha, Suvra S.; Naik, Akshata R.; Lewis, Kenneth T.; Naik, R.; Jena, Bhanu P.

doi:10.1016/j.micron.2016.10.005

Cited by 40 publications

(24 citation statements)

References 29 publications

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“…We chose DOX as our drug of choice due to the ease of analysis by the virtue of its absorption and bright fluorescence properties, and our data support its good antitumor activity in line with other published studies. [35][36][37] We observed that all the tested cell types were capable of shedding of DOX-loaded Exos; however, the highest efficacy was observed for PCCs. This superior efficiency of PCCs can be attributed to the efficiency of cancer cells to pumpout drugs in a more effective way than other cell types.…”

Section: Discussionmentioning

confidence: 85%

Drug-loaded exosomal preparations from different cell types exhibit distinctive loading capability, yield, and antitumor efficacies: a comparative analysis

Kanchanapally¹,

Deshmukh²,

Chavva³

et al. 2019

IJN

108

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BackgroundDespite tremendous advancement, cancer still remains one of the leading causes of death worldwide. Inefficiency of current drug delivery regimens is one important factor that limits the therapeutic efficacy of existing drugs, thus contributing to cancer mortality. To address this limitation, synthetic nanotechnology-based delivery systems have been developed; however, they raise concern of inducing adverse immunogenic reactions. Exosomes (Exos) are nonimmunogenic nanosized vesicles that have received significant attention as efficient drug delivery system.MethodsDrug loading in Exos were achieved by incubating different cell types viz pancreatic cancer cells (PCCs), pancreatic stellate cells (PSCs), and macrophages (MØs) with Doxorubicin (DOX). Differential ultracentrifugation was performed to isolate exosome and their size was determined by dynamic light scattering analysis. The efficacy of drug packaging into Exos was evaluated by HPLC. Flow cytometry was performed to examine the apoptosis. Cell viability was determined using the WST-1 assay.ResultsPCCs shed the most Exos and were the most efficient in drug loading followed by MØs and PSCs as examined by HPLC quantification. However, when compared for antitumor efficacy, MØ-derived Exos loaded with DOX (MØ-Exo-DOX) showed highest activity followed by PSCs and PCCs.ConclusionThese varying antitumor activities likely resulted from nondrug contents of Exos since we did not observe any significant differences in their uptake by the cancer cells. Altogether, our data suggest that donor cell-specific differences exist in Exos, which could influence their utility as drug carrier for therapeutic purposes.

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Section: Discussionmentioning

confidence: 85%

Drug-loaded exosomal preparations from different cell types exhibit distinctive loading capability, yield, and antitumor efficacies: a comparative analysis

Kanchanapally¹,

Deshmukh²,

Chavva³

et al. 2019

IJN

108

View full text Add to dashboard Cite

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“…A pancreatic cancer research shows that the chemotherapeutic adriamycin could be effectively transported and released in cancer cells by using superparamagnetic iron oxide NPs as carriers [18]. More inspiringly, a new administration route is discovered in brain tumor animal experiments.…”

Section: Evaluation Of Peg-mzf-np Gene Transfectionmentioning

confidence: 99%

Biological Characteristics and Carrier Functions of Pegylated Manganese Zinc Ferrite Nanoparticles

Guo

Dou

Lin

et al. 2019

Journal of Nanomaterials

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This study was performed to investigate the biocompatibility (BC), magnetothermal effect, and DNA binding biological characteristics of manganese zinc ferrite nanoparticles (Mn0.6Zn0.4Fe2O4-NPs (MZF-NPs)) coated with pegylated manganese (PEG-MZF-NPs). Their functions as gene transfer carrier for gene therapy and magnetic medium for tumor hyperthermia were also explored. The manganese zinc ferrite nanoparticles were synthesized through high temperature cracking, and their characterizations were discovered. Hemolysis test and MTT assay were performed to evaluate biocompatibility, and their self-heating effects in the alternating magnetic field were investigated. PEG-MZF-NPs with different concentrations were measured by using 7.0 T Micro-MR scanner (MRI) to calculate the T2 value and r2 relaxation rate of each sample. The CD44-shRNA plasmids were constructed, and their ability to bind PEG-MZF-NPs were examined. The DNA release from PEG-MZF-NP/DNA complex and protection of DNA from nuclease digestion were also detected. After CD44-shRNA-EGFP were transfected into the ovarian cancer SK-OV-3 cells by using PEG-MZF-NPs as carriers, the transfection efficiency was detected by a flow cytometer and expression of CD44 mRNA and protein in cells was detected using RT-PCR and Western blot, respectively. We successfully prepared PEG-MZF-NPs with favorable dispersity, magnetic responsiveness, and BC. Typically, the excellent magnetothermal effect can be used for a tumor magnetothermal therapeutic study. In vitro MRI showed the application potential for being magnetic resonance T2 relaxation contrast agents and the possibility to achieve goal of integration of targeting diagnosis and treatment. The CD44-shRNA plasmids have been successfully constructed and concluded that PEG-MZF-NPs may serve as gene transfer carriers for gene therapy.

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“…Moreover, they can produce the heating effect and kill 95% cells [49]. A research on pancreatic cancer showed that chemotherapeutic DOX can be rapidly transferred and released in pancreatic cancer cells by using superparamagnetic iron oxide (SPIO) NPs as the vectors monitored under electron microscope, X-ray, and optical microscope [50]. As we all know, most chemotherapeutics cannot pass the blood brain barrier (BBB).…”

Section: Research On Magnetic Nps As Vectorsmentioning

confidence: 99%

The Recent Advances of Magnetic Nanoparticles in Medicine

Guo

Lin

Huang

et al. 2018

Journal of Nanomaterials

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With the progress of nanotechnology and molecular biology, nanoparticles have been widely studied and applied in biomedicine. Particularly, characterized by unique magnetic property, targeting, and biocompatibility, magnetic nanoparticles have become one of the research hotspots in the nanomedical field. Herein, we summarized the recent advances of magnetic nanoparticles in medicine, including the property, carrier function, MRI, and tumor magnetic inductive hyperthermia of magnetic nanoparticles.

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Functionalized nanoparticles enable tracking the rapid entry and release of doxorubicin in human pancreatic cancer cells

Cited by 40 publications

References 29 publications

Drug-loaded exosomal preparations from different cell types exhibit distinctive loading capability, yield, and antitumor efficacies: a comparative analysis

Drug-loaded exosomal preparations from different cell types exhibit distinctive loading capability, yield, and antitumor efficacies: a comparative analysis

Biological Characteristics and Carrier Functions of Pegylated Manganese Zinc Ferrite Nanoparticles

The Recent Advances of Magnetic Nanoparticles in Medicine

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