Functionalized nanoparticle probes for protein detection

Park, Do Hyun; Lee, Jae Seung

doi:10.1007/s13391-014-4383-0

Cited by 8 publications

(5 citation statements)

References 84 publications

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“…PrSCs inherited the architecture of dye-sensitized solar cells (DSSCs), [15][16][17] most of which include a mesoscopic metal oxide layer (e.g., TiO 2 ), so the structure of early-stage PrSCs was similar to those of DSSCs, 14,18 and many studies still present mesoscopic PrSCs with very high PCEs. However, this type of PrSC cannot be developed as a flexible device because formation of mesoporous structure requires high temperature T > 450 °C, which damages most flexible plastic substrates.…”

Section: Introductionmentioning

confidence: 99%

Planar heterojunction organometal halide perovskite solar cells: roles of interfacial layers

Kim

Lim

Lee

2016

Energy Environ. Sci.

465

327

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Organometal halide perovskites are promising photo-absorption materials in solar cells due to high extinction coefficient, broad light absorption range and excellent semiconducting properties. The highest power conversion efficiency (PCE) of perovskite solar cells (PrSCs) is now 20.1%. However, a high-temperature processed mesoscopic metal oxide (e.g., TiO 2 ) must be removed to realize flexible PrSCs on plastic substrates using low temperature processes. Although the planar heterojunction (PHJ) structure can be considered as the most appropriate structure for flexible PrSCs, they have shown lower PCEs than those with a mesoscopic metal oxide layer. Therefore, development of interfacial layers is essential for achieving highly efficient PHJ PrSCs, and necessary in fabrication of flexible PrSCs. This review article gives an overview of progress in PHJ PrSCs and the roles of interfacial layers in the device, and suggests a practical strategy to fabricate highly efficient and flexible PHJ PrSCs. We conclude with our technical suggestion and outlook for further research direction.

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Section: Introductionmentioning

confidence: 99%

Planar heterojunction organometal halide perovskite solar cells: roles of interfacial layers

Kim

Lim

Lee

2016

Energy Environ. Sci.

465

327

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“…Nowadays, gold nanoparticles are extensively applied in chemical, biological, and biomedical research . The concept of nanoparticle‐assisted sample preparation, pre‐concentration, separation, and sensing plays important roles in many analytical procedures.…”

Section: Introductionmentioning

confidence: 99%

Functionalized gold nanoparticles for sample preparation: A review

Liu

Lämmerhofer

2019

Electrophoresis

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Sample preparation is a crucial step for the reliable and accurate analysis of both small molecule and biopolymers which often involves processes such as isolation, pre‐concentration, removal of interferences (purification), and pre‐processing (e.g., enzymatic digestion) of targets from a complex matrix. Gold nanoparticle (GNP)‐assisted sample preparation and pre‐concentration has been extensively applied in many analytical procedures in recent years due to the favorable and unique properties of GNPs such as size‐controlled synthesis, large surface‐to‐volume ratio, surface inertness, straightforward surface modification, easy separation requiring minimal manipulation of samples. This review article primarily focuses on applications of GNPs in sample preparation, in particular for bioaffinity capture and biocatalysis. In addition, their most common synthesis, surface modification and characterization methods are briefly summarized. Proper surface modification for GNPs designed in accordance to their target application directly influence their functionalities, e.g., extraction efficiencies, and catalytic efficiencies. Characterization of GNPs after synthesis and modification is worthwhile for monitoring and controlling the fabrication process to ensure proper quality and functionality. Parameters such as morphology, colloidal stability, and physical/chemical properties can be assessed by methods such as surface plasmon resonance, dynamic light scattering, ζ‐potential determinations, transmission electron microscopy, Taylor dispersion analysis, and resonant mass measurement, among others. The accurate determination of the surface coverage appears to be also mandatory for the quality control of functionality of the nanoparticles. Some promising applications of (functionalized) GNPs for bioanalysis and sample preparation are described herein.

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“…While small molecules confer such surface passivation, they typically offer limited control over the assembly structures of NPs. − Functionalizing NPs with biopolymers, particularly those having a degree of programmability, affords the capability to generate specific interactions. Complementary strands of DNA have been used to mediate assembly of metal NPs into well-defined small clusters, macroscopic aggregates, layered structures, − three-dimensional superlattices, , and complex nanostructures. − Of specific interest has been the control of NP spacing and reversible assembly. ,− DNA linkers can yield a broad range of interparticle separation distances ,, with assembly triggered by changes in system conditions. − The control over separation provided by DNA has been used to study distance dependent phenomena such as energy transfer between nanoparticles , and sensing DNA-binding proteins. − …”

Section: Introductionmentioning

confidence: 99%

Controlling Association and Separation of Gold Nanoparticles with Computationally Designed Zinc-Coordinating Proteins

et al. 2017

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Functionalization of nanoparticles with biopolymers has yielded a wide range of structured and responsive hybrid materials. DNA provides the ability to program length and recognition using complementary oligonucleotide sequences. Nature more often leverages the versatility of proteins, however, where structure, assembly, and recognition are more subtle to engineer. Herein, a protein was computationally designed to present multiple Zn coordination sites and cooperatively self-associate to form an antiparallel helical homodimer. Each subunit was unstructured in the absence of Zn or when the cation was sequestered with a chelating agent. When bound to the surface of gold nanoparticles via cysteine, the protein provided a reversible molecular linkage between particles. Nanoparticle association and changes in interparticle separation were monitored by redshifts in the surface plasmon resonance (SPR) band and by transmission electron microscopy (TEM). Titrations with Zn revealed sigmoidal transitions at submicromolar concentrations. The metal-ion concentration required to trigger association varied with the loading of the proteins on the nanoparticles, the solution ionic strength, and the cation employed. Specifying the number of helical (heptad) repeat units conferred control over protein length and nanoparticle separation. Two different length proteins were designed via extension of the helical structure. TEM and extinction measurements revealed distributions of nanoparticle separations consistent with the expected protein structures. Nanoparticle association, interparticle separation, and SPR properties can be tuned using computationally designed proteins, where protein structure, folding, length, and response to molecular species such as Zn can be engineered.

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Functionalized nanoparticle probes for protein detection

Cited by 8 publications

References 84 publications

Planar heterojunction organometal halide perovskite solar cells: roles of interfacial layers

Planar heterojunction organometal halide perovskite solar cells: roles of interfacial layers

Functionalized gold nanoparticles for sample preparation: A review

Controlling Association and Separation of Gold Nanoparticles with Computationally Designed Zinc-Coordinating Proteins

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