Functionalized large pore mesoporous silica nanoparticles for gene delivery featuring controlled release and co-delivery

Hartono, Sandy Budi; Truong, Nghia P.; Yu, Meihua; Jia, Zhongfan; Monteiro, Michael J.; Qiao, Shi Zhang; Yu, Chengzhong

doi:10.1039/c3tb21015d

Cited by 100 publications

(74 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…34 In follow-up studies they adsorbed the endosomolytic agent chloroquine into the pores while siRNA was attached via poly(2-dimethylaminoethyl methacrylate) (PDMAEMA) on the outside of the silica particles. 19 Good efficacies were still only reached when the LP-MSNs were equipped with covalently bound PEI instead of PDMAEMA. 35 In light of these collective findings we suggest that not only the pore size and surface charge are important in determining the loading capacity, but that the pore morphology might play a pivotal role in efficient siRNA release.…”

Section: Sirna Adsorption and Release Studies With Lp-msnmentioning

confidence: 99%

“…18 Other examples that used large-pore carriers for siRNA delivery needed the assistance of PEI to show good efficacy in gene knockdown. 19,20 Lin et al used 10 nm LP-MSN and cross-linked PDMAEMA via disulfide bonds to the host in order to achieve intracellular cleavage of the polymer and triggered siRNA delivery. They achieved silencing of luciferase and of the endogenous protein Lamin A/C of about 60% with low siRNA loadings of about 3 wt%.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Highly efficient siRNA delivery from core–shell mesoporous silica nanoparticles with multifunctional polymer caps

et al. 2016

View full text Add to dashboard Cite

A new general route for siRNA delivery is presented combining porous core-shell silica nanocarriers with a modularly designed multifunctional block copolymer. Specifically, the internal storage and release of siRNA from mesoporous silica nanoparticles (MSN) with orthogonal core-shell surface chemistry was investigated as a function of pore-size, pore morphology, surface properties and pH. Very high siRNA loading capacities of up to 380 µg per mg MSN were obtained with charge-matched amino-functionalized mesoporous cores, and release profiles show up to 80% siRNA elution after 24 h. We demonstrate that adsorption and desorption of siRNA is mainly driven by electrostatic interactions, which allow for high loading capacities even in medium-sized mesopores with pore diameters down to 4 nm in a stellate pore morphology. The negatively charged MSN shell enabled the association with a block copolymer containing positively charged artificial amino acids and oleic acid blocks, which acts simultaneously as capping and endosomal release agent. The potential of this multifunctional delivery platform is demonstrated by highly effective cell transfection and siRNA delivery into KB-cells. A luciferase reporter gene knock-down of up to 80-90% was possible using extremely low cell exposures with only 2.5 µg MSN containing 0.5 μg siRNA per 100 µL well.

show abstract

Section: Sirna Adsorption and Release Studies With Lp-msnmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Highly efficient siRNA delivery from core–shell mesoporous silica nanoparticles with multifunctional polymer caps

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Optimization of the synthetic parameters achieved varied controlled release kinetics of vancomycin enabling selection of the desired therapeutic profile (160 (176) and when using degradable poly (2-dimethylaminoethyl acrylate) controlled release of siRNA in to cells is achievable (174). However, the loading of large bimolecular genes into meso-pores of MSN is not straightforward, and many studies have shown DNA adsorption on the outer surface rather than encapsulation within pores (175,177).…”

Section: Ms Based Materials In Drug Deliverymentioning

confidence: 99%

Porous Inorganic Drug Delivery Systems—a Review

et al. 2017

View full text Add to dashboard Cite

Innovative methods and materials have been developed to overcome limitations associated with current drug delivery systems. Significant developments have led to the use of a variety of materials (as excipients) such as inorganic and metallic structures; marking a transition from conventional polymers. Inorganic materials, especially those possessing significant porosity, are emerging as good candidates for the delivery of a range of drugs (anti-biotics, anti-cancer and anti-inflammatories), providing several advantages in formulation and engineering (encapsulation of drug in amorphous form, controlled delivery and improved targeting). This review focuses on key selected developments in porous drug delivery systems. The review provides a short broad overview of porous polymeric materials for drug delivery before focusing on porous inorganic materials (e.g. Santa Barbara Amorphous (SBA) and Mobil Composition of Matter (MCM)) and their utilisation in drug dosage form development. Methods for their preparation and drug loading thereafter are detailed. Several examples of porous inorganic materials, drugs used and outcomes are discussed providing the reader with an understanding of advances in the field and realistic opportunities.

show abstract

“…Again, as expected, they obtained a similar behaviour with enhanced antiproliferative effect against KHOS cell line with combinations of CLQ and PLK or S10 siRNAs (Entry 7, Table 2). 163 Apart from intracellular Bcl-2 or ABC-transporters (such as Pgp) siRNAs, there have been also reported for interference therapies on angiogenesis. This, aimed to produce nutrient outage on the tumour areas, is also an interesting approach for future combination therapies.…”

Section: Please Do Not Adjust Marginsmentioning

confidence: 99%

Recent applications of the combination of mesoporous silica nanoparticles with nucleic acids: development of bioresponsive devices, carriers and sensors

Castillo¹,

Baeza²

2017

Biomater. Sci.

View full text Add to dashboard Cite

The discovery and control of the biological roles mediated by nucleic acids have turned them into a powerful tool for the development of advanced biotechnological materials. Much is the importance of those gene-keeping biomacromolecules that even nanomaterials have succumbed to the claimed benefits of DNA and RNA. Currently, there could be found in the literature a practically intractable number of examples which report the use in combination of nanoparticles with nucleic acids, which demands boundedness. Following this premise, this revision will only cover the most recent and powerful strategies developed to exploit the possibilities of nucleic acids as biotechnological materials when in combination with mesoporous silica nanoparticles. The extensive research done on nucleic acids has significantly incremented the technological possibilities for those biomacromolecules, which could be employed in many different applications; where substrate or sequence recognition or modulation of biological pathways due to its coding role in living cells are the most promising. In the present revision, the chosen counterpart, mesoporous silica nanoparticles, also with unique properties, became a reference material for drug delivery and biomedical applications due to their high biocompatibility and porous structure suitable for hosting and delivering small molecules. Although most of revisions deal with significant advances in the use of nucleic acid and mesoporous silica nanoparticles in biotechnological applications, a rationale classification of those new generation hybrid materials is still uncovered. Along this review there will be covered promising strategies for living cell and biological sensors, DNA-based molecular gates with targeting, transfection or silencing properties which could provide a significant advance of current nanomedicine.

show abstract

Functionalized large pore mesoporous silica nanoparticles for gene delivery featuring controlled release and co-delivery

Cited by 100 publications

References 33 publications

Highly efficient siRNA delivery from core–shell mesoporous silica nanoparticles with multifunctional polymer caps

Highly efficient siRNA delivery from core–shell mesoporous silica nanoparticles with multifunctional polymer caps

Porous Inorganic Drug Delivery Systems—a Review

Recent applications of the combination of mesoporous silica nanoparticles with nucleic acids: development of bioresponsive devices, carriers and sensors

Contact Info

Product

Resources

About