Functionalized human umbilical cord mesenchymal stem cells and injectable HA/Gel hydrogel synergy in endometrial repair and fertility recovery

Zhang, Donghai; Du, Qianqian; Li, C. K.; Ding, Chuanfeng; Chen, Junfeng; He, Yun; Duan, Tao; Feng, Qian; Yu, Yongsheng; Zhou, Qian

doi:10.1016/j.actbio.2023.06.013

Cited by 13 publications

(4 citation statements)

References 49 publications

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“…Embedded active proteins and estrogen in bio-scaffolds endow them with antioxidant properties that protect against fibrosis within the uterine cavity and encourage the self-repair of the remaining endometrial cells by downregulating excessive oxidative stress and fibrosis-related mediators [ [153] , [154] , [155] , [156] , [157] ]. Administration of both SCs and their derivatives, such as human umbilical cord mesenchymal SCs (UCMSCs), mesenchymal SCs (MSCs), apoptotic bodies, and secretomes, in hydrogels and acellular scaffolds also potentially contribute to satisfactory endothelial regeneration after IUA [ [57] , [158] , [159] ]. Collagen, a practical framework for promoting wound healing and tissue repair, has been applied to acellular scaffolds [ 160 ].…”

Section: Application Of Bioengineering Technology In Endometrial Dise...mentioning

confidence: 99%

Bioengineering approaches for the endometrial research and application

Dai,

Liang,

Guo

et al. 2024

Materials Today Bio

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Section: Application Of Bioengineering Technology In Endometrial Dise...mentioning

confidence: 99%

Bioengineering approaches for the endometrial research and application

Dai,

Liang,

Guo

et al. 2024

Materials Today Bio

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“…Vascular endothelial growth factor (VEGF) signaling plays a key role in blood vessel formation and growth, stimulating angiogenesis, promoting endothelial cell proliferation and migration, and increasing vascular permeability (Zhang et al, 2023). Compared with the control group, all VEGF intensities in the wound dressing group increased to some extent.…”

Section: Angiogenesis and Inflammatory Response In Wound Healingmentioning

confidence: 99%

Antimicrobial peptide-modified AIE visual composite wound dressing for promoting rapid healing of infected wounds

Chen,

Qian,

Peng

et al. 2024

Front. Bioeng. Biotechnol.

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Wound infection is a major problem faced during wound healing. Therefore, it is necessary to develop wound dressings with excellent antimicrobial properties. Here, a smart response system of PVA-TPE/HA-AMP/SF/ALG wound dressing was prepared by a combination of chemical cross-linking and freeze-drying methods. We grafted AMP onto HA to endow the wound dressing with bacterial resistance and slow release of AMP. At the same time, the system detects bacterial activity in real time for precise antimicrobial activity (through the use of PVA-TPE) and modulates inflammation to reduce bacterial infection (through the use of AMP). In addition, the PVA-TPE/HA-AMP/SF/ALG wound dressing has a good three-dimensional mesh structure, which promotes cell proliferation, enhances collagen deposition and angiogenesis, and thus effectively promotes rapid healing of infected wounds. Moreover, it can induce the expression of inflammatory factors such as VEGF, TNF-α, IFN-γ, IL-4 and TGF-β1 in infected wounds through the Wnt/CAMK/p-PKC signaling pathway, inhibit inflammatory responses, promote wound healing and reduce scar formation. Therefore, the PVA-TPE/HA-AMP/SF/ALG wound dressing smart response system shows great promise in infected wound healing.

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“…MSCs have many advantages, including low immunogenicity, homing to the site of injury, anti-inflammatory activity, and paracrine profiles ( 5 , 6 ). Numerous animal experiments have demonstrated that MSCs have a great therapeutic effect in attenuating endometrial fibrosis, increasing the number of endometrial glands, and promoting vascular regeneration ( 7 – 9 ). Furthermore, MSC transplantation for IUA has entered phase I clinical trials.…”

Section: Introductionmentioning

confidence: 99%

“…This microenvironment can simulate natural cell-cell, cellular extracellular matrix, and cell-soluble factor interactions ( 14 ). Current studies have demonstrated that various bio-scaffolds, such as hydrogels, collagen, and poly (glycerol sebacate), combined with MSCs can effectively repair the morphology and function of the endometrium ( 9 , 15 , 16 ). In addition, 3D-printed biomaterials have been widely used to mimic histological architecture and functions of endometrial tissue.…”

Section: Introductionmentioning

confidence: 99%

Validity of stem cell-loaded scaffolds to facilitate endometrial regeneration and restore fertility: a systematic review and meta-analysis

Huang,

Zheng,

Shi

et al. 2024

Front. Endocrinol.

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ObjectiveVarious stem cell-loaded scaffolds have demonstrated promising endometrial regeneration and fertility restoration. This study aimed to evaluate the efficacy of stem cell-loaded scaffolds in treating uterine injury in animal models.MethodsThe PubMed, Embase, Scopus, and Web of Science databases were systematically searched. Data were extracted and analyzed using Review Manager version 5.4. Improvements in endometrial thickness, endometrial glands, fibrotic area, and number of gestational sacs/implanted embryos were compared after transplantation in the stem cell-loaded scaffolds and scaffold-only group. The standardized mean difference (SMD) and confidence interval (CI) were calculated using forest plots.ResultsThirteen studies qualified for meta-analysis. Overall, compared to the scaffold groups, stem cell-loaded scaffolds significantly increased endometrial thickness (SMD = 1.99, 95% CI: 1.54 to 2.44, P < 0.00001; I² = 16%) and the number of endometrial glands (SMD = 1.93, 95% CI: 1.45 to 2.41, P < 0.00001; I² = 0). Moreover, stem cell-loaded scaffolds present a prominent effect on improving fibrosis area (SMD = −2.50, 95% CI: –3.07 to –1.93, P < 0.00001; I² = 36%) and fertility (SMD = 3.34, 95% CI: 1.58 to 5.09, P = 0.0002; I² = 83%). Significant heterogeneity among studies was observed, and further subgroup and sensitivity analyses identified the source of heterogeneity. Moreover, stem cell-loaded scaffolds exhibited lower inflammation levels and higher angiogenesis, and cell proliferation after transplantation.ConclusionThe evidence indicates that stem cell-loaded scaffolds were more effective in promoting endometrial repair and restoring fertility than the scaffold-only groups. The limitations of the small sample sizes should be considered when interpreting the results. Thus, larger animal studies and clinical trials are needed for further investigation.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO, identifier CRD42024493132.

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Functionalized human umbilical cord mesenchymal stem cells and injectable HA/Gel hydrogel synergy in endometrial repair and fertility recovery

Cited by 13 publications

References 49 publications

Bioengineering approaches for the endometrial research and application

Bioengineering approaches for the endometrial research and application

Antimicrobial peptide-modified AIE visual composite wound dressing for promoting rapid healing of infected wounds

Validity of stem cell-loaded scaffolds to facilitate endometrial regeneration and restore fertility: a systematic review and meta-analysis

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