Functionalized collagen-silver nanocomposites for evaluation of the biocompatibility and vascular differentiation capacity of mesenchymal stem cells

Chang, Kai-Bo; Shen, Chiung‐Chyi; Hsu, Shan‐hui; Tang, Cheng‐Ming; Yang, Yi‐Chin; Liu, Sin-Ying; Ku, Tian Ren; Kung, Mei-Lang; Hsieh, Hsien-Hsu; Hung, Huey-Shan

doi:10.1016/j.colsurfa.2021.126814

Cited by 9 publications

(6 citation statements)

References 60 publications

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“…For instance, Ag nanoparticles have verified to be a potential anti-microbial nanomaterial [ 43 ]. Our research team have developed the nanocomposites fabricating Ag nanoparticles with Col, which demonstrated the longer stress fiber extension for MSCs adhesion and migration [ 44 ]. Additionally, Au nanoparticles were proved to be non-cytotoxic, which owned superior biocompatibility for biomedical applications such as vascular regeneration [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Biocompatibility and Endothelial Differentiation Capacity of Mesenchymal Stem Cells by Polyethylene Glycol Nanogold Composites

et al. 2021

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Cardiovascular Diseases (CVDs) such as atherosclerosis, where inflammation occurs in the blood vessel wall, are one of the major causes of death worldwide. Mesenchymal Stem Cells (MSCs)-based treatment coupled with nanoparticles is considered to be a potential and promising therapeutic strategy for vascular regeneration. Thus, angiogenesis enhanced by nanoparticles is of critical concern. In this study, Polyethylene Glycol (PEG) incorporated with 43.5 ppm of gold (Au) nanoparticles was prepared for the evaluation of biological effects through in vitro and in vivo assessments. The physicochemical properties of PEG and PEG–Au nanocomposites were first characterized by UV-Vis spectrophotometry (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), and Atomic Force Microscopy (AFMs). Furthermore, the reactive oxygen species scavenger ability as well as the hydrophilic property of the nanocomposites were also investigated. Afterwards, the biocompatibility and biological functions of the PEG–Au nanocomposites were evaluated through in vitro assays. The thin coating of PEG containing 43.5 ppm of Au nanoparticles induced the least platelet and monocyte activation. Additionally, the cell behavior of MSCs on PEG–Au 43.5 ppm coating demonstrated better cell proliferation, low ROS generation, and enhancement of cell migration, as well as protein expression of the endothelialization marker CD31, which is associated with angiogenesis capacity. Furthermore, anti-inflammatory and endothelial differentiation ability were both evaluated through in vivo assessments. The evidence demonstrated that PEG–Au 43.5 ppm implantation inhibited capsule formation and facilitated the expression of CD31 in rat models. TUNEL assay also indicated that PEG–Au nanocomposites would not induce significant cell apoptosis. The above results elucidate that the surface modification of PEG–Au nanomaterials may enable them to serve as efficient tools for vascular regeneration grafts.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Biocompatibility and Endothelial Differentiation Capacity of Mesenchymal Stem Cells by Polyethylene Glycol Nanogold Composites

et al. 2021

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“…The human umbilical cord Wharton’s jelly mesenchymal stem cells (MSCs) were applied for the present research, which were also selected in our previous articles [ 9 , 34 ]. The cells were stored after being cultured in H-DMEM medium (Invitrogen, Waltham, MA, USA) containing 10% fetal bovine serum (FBS) and 1% ( v/v ) antibiotics (100 U/mL penicillin/streptomycin).…”

Section: Methodsmentioning

confidence: 99%

“…Inorganic nanomaterials such as graphene oxide [ 8 ], gold, silver [ 9 ] and copper [ 10 ] nanoparticles have been widely investigated in biomedicine studies. Gold nanoparticle (AuNP) has been proved to be a biocompatible nano-metallic material, which has been widely applied in various fields, including bioimaging, drug delivery core systems and surface modification techniques [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Applications of Mesenchymal Stem Cell Loaded with Gold Nanoparticles for Regenerative Medicine

et al. 2023

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In the present study, the various concentrations of AuNP (1.25, 2.5, 5, 10 ppm) were prepared to investigate the biocompatibility, biological performances and cell uptake efficiency via Wharton’s jelly mesenchymal stem cells and rat model. The pure AuNP, AuNP combined with Col (AuNP-Col) and FITC conjugated AuNP-Col (AuNP-Col-FITC) were characterized by Ultraviolet–visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR) and Dynamic Light Scattering (DLS) assays. For in vitro examinations, we explored whether the Wharton’s jelly MSCs had better viability, higher CXCR4 expression, greater migration distance and lower apoptotic-related proteins expression with AuNP 1.25 and 2.5 ppm treatments. Furthermore, we considered whether the treatments of 1.25 and 2.5 ppm AuNP could induce the CXCR4 knocked down Wharton’s jelly MSCs to express CXCR4 and reduce the expression level of apoptotic proteins. We also treated the Wharton’s jelly MSCs with AuNP-Col to investigate the intracellular uptake mechanisms. The evidence demonstrated the cells uptake AuNP-Col through clathrin-mediated endocytosis and the vacuolar-type H+-ATPase pathway with good stability inside the cells to avoid lysosomal degradation as well as better uptake efficiency. Additionally, the results from in vivo examinations elucidated the 2.5 ppm of AuNP attenuated foreign body responses and had better retention efficacy with tissue integrity in animal model. In conclusion, the evidence demonstrates that AuNP shows promise as a biosafe nanodrug delivery system for development of regenerative medicine coupled with Wharton’s jelly MSCs.

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“…Composite gels consisting of collagen and silver help to eliminate infection of skin wounds and promote the survival and growth of skin-related cells (fibroblasts, endothelial cells, keratinocytes, etc.). Previous studies have introduced Col-Ag nanocomposites through surface modification for the clinical treatment of vascular regeneration [65](Fig. 2).…”

Section: Combination With Inorganic Antimicrobialsmentioning

confidence: 99%

Recent advances of collagen composite biomaterials for biomedical engineering: antibacterial functionalization and 3D-printed architecturalization

Zheng,

Tseomashko,

Voronova

et al. 2024

Collagen & Leather

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Collagen possesses high biocompatibility with all tissue and cell types in the body, enabling the creation of multifunctional composite materials for medical applications. In biomedical engineering, naturally-sourced collagen is often combined with diverse organic and inorganic bioactive components to eliminate defects and disorders in fields including orthopedics, dermatology, and more. At the same time, medical-related infection issues and the precise treatment needs of patients require collagen composite biomaterials to have antibacterial properties and customized structures. This paper reviews the antibacterial functionalization of collagen composite biomaterials in recent years, including the combination with inorganic or organic antibacterial agents, which is beneficial for preventing and controlling biological contamination in medical applications. Then, the existing problems and future development directions for the architecturalization of collagen composite materials with 3D printing were discussed, providing guidance for personalized customization of multifunctional materials to meet the specific needs of patients in the future. Graphical Abstract

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Functionalized collagen-silver nanocomposites for evaluation of the biocompatibility and vascular differentiation capacity of mesenchymal stem cells

Cited by 9 publications

References 60 publications

Evaluation of the Biocompatibility and Endothelial Differentiation Capacity of Mesenchymal Stem Cells by Polyethylene Glycol Nanogold Composites

Evaluation of the Biocompatibility and Endothelial Differentiation Capacity of Mesenchymal Stem Cells by Polyethylene Glycol Nanogold Composites

Therapeutic Applications of Mesenchymal Stem Cell Loaded with Gold Nanoparticles for Regenerative Medicine

Recent advances of collagen composite biomaterials for biomedical engineering: antibacterial functionalization and 3D-printed architecturalization

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