Functionalized Collagen Scaffold Neutralizing the Myelin-Inhibitory Molecules Promoted Neurites Outgrowth in Vitro and Facilitated Spinal Cord Regeneration in Vivo

Tang

et al. 2016

Sci. China Life Sci.

Self Cite

The objective of this clinical study was to assess the safety and feasibility of the collagen scaffold, NeuroRegen scaffold, one year after scar tissue resection and implantation. Scar tissue is a physical and chemical barrier that prevents neural regeneration. However, identification of scar tissue is still a major challenge. In this study, the nerve electrophysiology method was used to distinguish scar tissue from normal neural tissue, and then different lengths of scars ranging from 0.5-4.5 cm were surgically resected in five complete chronic spinal cord injury (SCI) patients. The NeuroRegen scaffold along with autologous bone marrow mononuclear cells (BMMCs), which have been proven to promote neural regeneration and SCI recovery in animal models, were transplanted into the gap in the spinal cord following scar tissue resection. No obvious adverse effects related to scar resection or NeuroRegen scaffold transplantation were observed immediately after surgery or at the 12-month follow-up. In addition, patients showed partially autonomic nervous function improvement, and the recovery of somatosensory evoked potentials (SSEP) from the lower limbs was also detected. The results indicate that scar resection and NeuroRegen scaffold transplantation could be a promising clinical approach to treating SCI.NeuroRegen scaffold, chronic spinal cord injury, scar resection, collagen scaffold transplantation, bone marrow mononuclear cells, tissue regeneration Citation:Xiao, Z., Tang, F., Tang, J., Yang, H., Zhao, Y., Chen, B., Han, S., Wang, N., Li, X., Cheng, S., Han, G., Zhao, C., Yang, X., Chen, Y., Shi, Q., Hou, S., Zhang, S., and Dai, J. (2016). One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients. Sci China Life Sci 59, 647 -655.

Section: Discussionmentioning

confidence: 99%

One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients

Tang

et al. 2016

Sci. China Life Sci.

Self Cite

“…CBD is a heptapeptide (TKKTLRT) derived from collagenase and can specifically bind to collagen I (de Souza & Brentani, ). CBD‐fused proteins have been expressed to bind collagen scaffolds to facilitate spinal cord regeneration and to bind to collagen gel to recruit endogenous stem cells for myocardial infarction treatment (Li et al, ; Sun et al, ). The immunoblotting assay proved the high expression levels of collagen I and other main ECM components such as fibronectin and laminin in liquid ECM we prepared.…”

Section: Discussionmentioning

confidence: 99%

The extracellular matrix enriched with membrane metalloendopeptidase and insulin‐degrading enzyme suppresses the deposition of amyloid‐beta peptide in Alzheimer's disease cell models

Zhang

et al. 2019

J Tissue Eng Regen Med

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Amyloid plaque is a typical feature of Alzheimer's disease (AD) and is one of the targets for AD therapy. Membrane metalloendopeptidase (MME) and insulin‐degrading enzyme (IDE) are two types of proteases that could cleave beta‐amyloid (Aβ) peptides generated by neuron cells of AD patients. Extracellular matrix (ECM) plays a crucial role in regulating tissue‐specific functions and is an ideal biomaterial for tissue repair. In this study, we extracted the liquid ECM enriched with collagen‐binding‐domain‐fused IDE or MME from human foreskin fibroblast cells. We found that these ECM biomaterials reduced the aggregation of Aβ peptides, prevented the formation of amyloid plaques, and also suppressed phosphorylation of Tau protein in AD cell models. Overall, our research provides a novel ECM biomaterial that can be potentially used for AD therapy.

“…A CBD derived from collagenase was used for enhancing the binding ability of EGFR‐Fab with collagen scaffold. In the past several years, multiple growth factors were fused with CBD to achieve controlled release from collagen scaffold . Our previous study also demonstrated that CBD‐EGFR‐Fab increased the binding ability with collagen for target cancer therapy .…”

Section: Discussionmentioning

confidence: 99%

A Dual Functional Scaffold Tethered with EGFR Antibody Promotes Neural Stem Cell Retention and Neuronal Differentiation for Spinal Cord Injury Repair

Bai

Zhao

Adv Healthcare Materials

et al. 2017

Self Cite

workers fabricate a dual functional collagen scaffold tethered with a collagen-binding epi-dermal growth factor receptor (EGFR) antibody in article number 1601279. This scaffold can simultaneously attract neural stem cells (NSCs) and promote their neuronal differentiation after spinal cord injury by specifically binding to EGFR molecule expressed on NSCs and attenuating EGFR signaling, which is responsible for the inhibition of differentiation of NSCs toward neurons. Blood Oxygen Measurement In article number 1601013, Xue Feng and co-workers report a systematic strategy to design an epidermal inorganic optoelectronic device by using specific strain-isolation design, nano-diamond thinning and hybrid transfer printing. This device has similar mechanical properties to epidermis and it is totally conformal to skin and keeps the constant light transmission between emitting element and photodetector as well as the signal stability due to skin deformation. It is also utilized to quantitatively detect human peripheral capillary oxygen saturation and cardiac pulse rate in vitro experiment. This strategy opens a route for traditional inorganic optoelectronics to achieve flexibility and stretchability and improve the performance of optoelec-tronics for biomedical application. Small is the very best interdisciplinary forum for all experimental and theoretical aspects of fundamental and applied research at the micro and nano length scales. With an ISI Impact Factor of 8.315 and publishing every week with papers online in advance of print, Small is your first-choice venue for top-quality communications, detailed full papers, cutting-edge concepts, and in-depth reviews of all things micro and nano. www.small-journal.com In article number 1700002, Giada Graziana Genti, Gianni Ciofani, and co-workers describe the most recent and exciting developments related to the field of nanomedi-cine that relies on the control of cell functions through physical remote manipulation mediated by smart nano-particles. Piezoelectric materials with micrometric and nanometric surface structuring provide smart interfaces to actively tune biological environment responses towards a favourite phenotype. Here, high-pressure hydrothermal conversion of corrugated titanium dioxide into barium titanate produces repeated microstructures mimicking Phalaenopsis genus morphology, working as anchoring points for neurons and active sites for voltage-gated calcium channel opening under mechanical stimulation. Particulate embolization is used to block blood vessels, particularly within hyper-vascularized tumors, as they are available in calibrated sizes and can be delivered distally via microcatheters for precise occlusion with optional locoregional drug delivery. The current progress in development of bespoke techniques incorporating physical handling, fluid dynamics, occlusive behavior, and sustained drug elution kinetics within vascular systems is reviewed. Smart nanoparticles are exploited for remote cell stimulation. The most recent and exciting findings about...