Functionalization of β-lactam antibiotic on lysozyme capped gold nanoclusters retrogress MRSA and its persisters following awakening

Kalita, Sanjeeb; Kandimalla, Raghuram; Bhowal, Ashim Chandra; Kotoky, Jibon; Kundu, Sarathi

doi:10.1038/s41598-018-22736-5

Cited by 69 publications

(48 citation statements)

References 56 publications

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“…S. Kalita et al . 86 reported that AuNPs are biocompatible towards mammalian cells and have antibacterial properties. As metallic nanoparticles, they are used in small doses because of their controlled release effect, thereby diminish any proposed cytotoxicity by reducing the necessity for high doses.…”

Section: Discussionmentioning

confidence: 99%

Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents

Arafa

El-Kased

Elmazar

2018

Sci Rep

184

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Thermoresponsive gels containing gold nanoparticles (AuNPs) were prepared using Pluronic®127 alone (F1) and with hydroxypropyl methylcellulose (F2) at ratios of 15% w/w and 15:1% w/w, respectively. AuNPs were evaluated for particle size, zeta-potential, polydispersity index (PDI), morphology and XRD pattern. AuNP-containing thermoresponsive gels were investigated for their gelation temperature, gel strength, bio-adhesive force, viscosity, drug content, in vitro release and ex-vivo permeation, in addition to in vitro antibacterial activity against bacteria found in burn infections, Staphylococcus aureus. In vivo burn healing and antibacterial activities were also investigated and compared with those of a commercial product using burn-induced infected wounds in mice. Spherical AuNPs sized 28.9–37.65 nm displayed a surface plasmon resonance band at 522 nm, a PDI of 0.461, and a zeta potential of 34.8 mV with a negative surface charge. F1 and F2 showed gelation temperatures of 37.2 °C and 32.3 °C, bio-adhesive forces of 2.45 ± 0.52 and 4.76 ± 0.84 dyne/cm2, viscosities of 10,165 ± 1.54 and 14,213 ± 2.31 cP, and gel strengths between 7.4 and 10.3 sec, respectively. The in vitro release values of F1 and F2 were 100% and 98.03% after 6 h, with permeation flux values of (J1) 0.2974 ± 2.85 and (J2) 0.2649 ± 1.43 (µg/cm2·h), respectively. The formulations showed antibacterial activity with the highest values for wound healing properties, as shown in vivo and by histopathological studies. This study demonstrates that a smart AuNPs thermoresponsive gel was successful as an antibacterial and wound healing transdermal drug delivery system.

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Section: Discussionmentioning

confidence: 99%

Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents

Arafa

El-Kased

Elmazar

2018

Sci Rep

184

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“…One of the reasons was possibly caused by their antibacterial effect. 52 This antibacterial effect of Lys-AuNCs during the proliferation of osteoblast will be further investigated in the future. Meanwhile, they increased protein expressions such as Col-1 and ALP, which can promote PI3K/Akt signaling pathways.…”

Section: Inhibition Of Osteoclastogenesismentioning

confidence: 96%

<p>Ultra-Small Lysozyme-Protected Gold Nanoclusters as Nanomedicines Inducing Osteogenic Differentiation</p>

Li¹,

Zhuang²,

Tao³

et al. 2020

IJN

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Ultra-small gold nanoclusters (AuNCs), as emerging fluorescent nanomaterials with excellent biocompatibility, have been widely investigated for in vivo biomedical applications. However, their effects in guiding osteogenic differentiation have not been investigated, which are important for osteoporosis therapy and bone regeneration. Herein, for the first time, lysozymeprotected AuNCs (Lys-AuNCs) are used to stimulate osteogenic differentiation, which have the potential for the treatment of bone disease. Methods: Proliferation of MC3T3E-1 is important for osteogenic differentiation. First, the proliferation rate of MC3T3E-1 was studied by Cell Counting Kit-8 (CCK8) assays. Signaling pathways of PI3K/Akt play central roles in controlling proliferation throughout the body. The expression of PI3K/Akt was investigated in the presence of lysozyme, and lysozyme-protected AuNCs (Lys-AuNCs) by Western blot (WB) and intracellular cell imaging to evacuate the osteogenic differentiation mechanisms. Moreover, the formation of osteoclasts (OC) plays a negative role in the differentiation of osteoblasts. Nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) signaling pathways are used to understand the negative influence of the osteogenic differentiation by the investigation of Raw 264.7 cell line. Raw 264.7 (murine macrophage-like) cells and NIH/3T3 (mouse fibroblast) cells were treated with tyloxapol, and the cell viability was assessed. Raw 264.7 cells have also been used for in vitro studies, on understanding the osteoclast formation and function. The induced osteoclasts were identified by TRAP confocal fluorescence imaging. These key factors in osteoclast formation, such as (NFATc-1, c-Fos, V-ATPase-2 and CTSK), were investigated by Western blot. Results: Based on the above investigation, Lys-AuNCs were found to promote osteogenic differentiation and decrease osteoclast activity. It is noteworthy that the lysozyme (protected template), AuNPs, or the mixture of Lysozyme and AuNPs have negligible effects on osteoblastic differentiation compared to Lys-AuNCs. Conclusion: This study opens up a novel avenue to develop a new gold nanomaterial for promoting osteogenic differentiation. The possibility of using AuNCs as nanomedicines for the treatment of osteoporosis can be expected.

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“…In addition, some Au NCs possess inherent antibacterial activity, and may exert certain polyvalent and synergistic effects through antibiotic loading to enhance the antibacterial activity of nanosystems . For example, Kalita et al developed a potent antibacterial hybrid prepared through surface functionalization of lysozyme-capped Au NCs (Lys-Au NCs) with β-lactam antibiotic ampicillin (Lys-Au NCs-Amp) (Kalita et al, 2018). The antibacterial hybrid not only reverses the MRSA resistance toward ampicillin but also exhibits enhanced antimicrobial activity against non-resistant bacterial strains.…”

Section: Antibiotic-au Ncs Systemsmentioning

confidence: 99%

Gold Nanoclusters for Bacterial Detection and Infection Therapy

et al. 2020

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Infections caused by antibiotic-resistant bacteria have become one of the most serious global public health crises. Early detection and effective treatment can effectively prevent deterioration and further spreading of the bacterial infections. Therefore, there is an urgent need for time-saving diagnosis as well as therapeutically potent therapy approaches. Development of nanomedicine has provided more choices for detection and therapy of bacterial infections. Ultrasmall gold nanoclusters (Au NCs) are emerging as potential antibacterial agents and have drawn intense attention in the biomedical fields owing to their excellent biocompatibility and unusual physicochemical properties. Recent significant efforts have shown that these versatile Au NCs also have great application potential in the selective detection of bacteria and infection treatment. In this review, we will provide an overview of research progress on the development of versatile Au NCs for bacterial detection and infection treatment, and the mechanisms of action of designed diagnostic and therapeutic agents will be highlighted. Based on these cases, we have briefly discussed the current issues and perspective of Au NCs for bacterial detection and infection treatment applications.

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Functionalization of β-lactam antibiotic on lysozyme capped gold nanoclusters retrogress MRSA and its persisters following awakening

Cited by 69 publications

References 56 publications

Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents

Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents

<p>Ultra-Small Lysozyme-Protected Gold Nanoclusters as Nanomedicines Inducing Osteogenic Differentiation</p>

Gold Nanoclusters for Bacterial Detection and Infection Therapy

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