Functionalization of Ti substrate with pH‐responsive naringin‐ZnO nanoparticles for the reconstruction of large bony after osteosarcoma resection

Abstract

After bone tumor resection, the large bony deficits are commonly reconstructed with Ti‐based metallic endoprosthesis, which provide immediate stable fixation and allow early ambulation and weight bearing. However, when used in osteosarcoma resection, Ti implant‐relative infection and tumor recurrence were recognized as the two critical factors for implantation failure. Hence, in this work, a novel zinc oxide nanoparticle decorating with naringin was prepared and immobilized onto Ti substrate. The drugs delivery profiles proved that in the bacterial infection and Warburg effect of osteosarcoma‐induced acidic condition, naringin and Zn2+ can be released easily from the functional Ti substrate. The anti‐osteosarcoma and antibacterial assay showed the delivered naringin and Zn2+ can induce a remarkable increase of oxidative stress in bacteria (Escherichia coli and Staphylococcus aureus) and osteosarcoma (Saos‐2 cells) by producing reactive oxygen species (ROS). Accumulation of ROS results in damage of bacterial biofilm and bacterial membrane, leading to the leakage of bacterial RNA and DNA. Meanwhile, the increase of ROS induces osteosarcoma cell apoptosis by activating ROS/extracellular signal‐regulated kinase signaling pathway. Furthermore, in vitro cellular experiments, including cell viability, alkaline phosphatase activity, collagen secretion, extracellular matrix mineralization level, indicated that the functional Ti substrate exhibited great potential for osteoblasts proliferation and differentiation. Hence, this study provides a simple and promising strategy of developing multifunctional Ti‐based implants for the reconstruction of large bony after osteosarcoma resection.

Zinc oxide nanoparticles conjugated with clinically-approved medicines as potential antibacterial molecules

“…Shifting of peaks was observed at 1639 cm −1 and 1446 cm −1 which are attributed to the stretching frequencies of C = O and –C = C– (aromatic) bond. Peak at 1080 cm −1 is the stretching vibration of –C–O– (polyols) groups of Naringin in NAR-ZnO-BCD NPs (Yang et al 2020 ). The peak shifting observed in the absorption frequencies of C = O and –C–O– bond indicated the interaction of these functional groups with the surface of ZnO-BCD NPs (Fig.…”

“…The data are representative of several experiments performed in duplicate and expressed as the mean ± standard error, where (*) represent when P ≤ 0.05 loaded with β-cyclodextrin and five drugs (Quericitin, Ceftrixon, Naranjin, Amphotericin B and Ampicillin). These formulations were characterized using a range of techniques such as zeta potential analysis and zeta sizer, UV-visible, FTIR, AFM as previously described (Gagoś and Arczewska 2010;Ethiraj et al 2014;Catauro et al 2015;Liu et al 2015;Diaz-Uribe et al 2016;Kalaiselvi et al 2016;Kavitha et al 2017;Mushtaq et al 2017;Reddy et al 2020;Kadam et al 2020;Kaur et al 2020;Yang et al 2020;Sathishkumar et al 2021). ZnO-NPs were synthesized using direct precipitation method and upon successful formation coupled with β-cycldextrin.…”

“…4b). After loading of Naringin over the surface of ZnO-BCD NPs, the FTIR spectra of NAR-ZnO-BCD NPs showed broad peak in the region of 3500-3300 cm −1 corresponding to the -OH group while peak at 2930 cm −1 is due to the stretching vibration of C-H bond (Yang et al 2020) (Fig. 4c).…”

A Systematic Review of the Preventive and Therapeutic Effects of Naringin Against Human Malignancies

“…Ti-naringin-3-carboxyphenylboronic acid (PBA)-zinc oxide (ZnO) nanoparticles reduce infection and tumor recurrence and induce Saos-2 osteosarcoma cells apoptosis through activation of ROS/ERK signaling. In vitro cellular experiments showed that these nanoparticles could promote the proliferation and growth of osteoblasts ( Yang et al, 2020 ). Nevertheless, in vivo experiments are required for understanding the anticancer properties of Ti-Naringin-PBA-ZnO nanoparticles in osteosarcoma.…”

Knockdown of lncRNA FEZF1-AS1 Inhibits metastasis of Osteosarcoma Cells by miR-4456/GALNT10

“…OS is the most frequent bone tumor in children and teenagers with high fatality rates [1]. Despite the much effort spent over the decades, the overall survival of OS patients remains dismal [2].…”