Functionalization of silicon dioxide and silicon nitride surfaces with aminosilanes for optical biosensing applications

Αντωνίου, Μαρία; Tsounidi, Dimitra; Petrou, Panagiota; Beltsios, K.; Kakabakos, Sotirios

doi:10.1002/mds3.10072

Cited by 20 publications

(20 citation statements)

References 32 publications

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“…30,31 Moreover, it has already been demonstrated that the thermal treatment at the temperature of 120 °C is crucial to promote the covalent binding of APTES molecules to the hydrophilic surface. 32 We found that, among the different samples analyzed, the surfaces obtained by solution phase deposition of APTES, followed by annealing, and dip coating deposition of CMD, or vapor phase deposition of APTES, followed by annealing treatment, and spin or dip coating deposition of CMD yielded to highest luminescence values (Figure 2b), indicating that the highest level of protein immobilization was reached with these fabrication workflows. Although dip coating is the preferential approach for complex patterns, spin coating resulted in faster and more reliable CMD deposition, including thickness control on the deposited films.…”

Section: ■ Results and Discussionmentioning

confidence: 90%

“…Moreover, we detected a positive effect of the annealing treatment, performed after the APTES deposition, in all the conditions that we tested. The vacuum thermal process, entailed by the annealing treatment, is capable to promote the selective removal of APTES and OTS molecules that are physiosorbed on the surface but not chemically bound, enhancing the subsequent reproducible deposition of the CMD. , Moreover, it has already been demonstrated that the thermal treatment at the temperature of 120 °C is crucial to promote the covalent binding of APTES molecules to the hydrophilic surface . We found that, among the different samples analyzed, the surfaces obtained by solution phase deposition of APTES, followed by annealing, and dip coating deposition of CMD, or vapor phase deposition of APTES, followed by annealing treatment, and spin or dip coating deposition of CMD yielded to highest luminescence values (Figure b), indicating that the highest level of protein immobilization was reached with these fabrication workflows.…”

Section: Resultsmentioning

confidence: 91%

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Patterned Carboxymethyl-Dextran Functionalized Surfaces Using Organic Mixed Monolayers for Biosensing Applications

Ambrosetti

Conti

Teixeira

et al. 2022

ACS Appl. Bio Mater.

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The deposition of biomolecules on biosensing surface platforms plays a key role in achieving the required sensitivity and selectivity for biomolecular interactions analysis. Controlling the interaction between the surface and biomolecules is increasingly becoming a crucial design tool to modulate the surface properties needed to improve the performance of the assay and the detection outcome. Carboxymethyl-dextran (CMD) coating can be exploited to promote chemical grafting of proteins, providing a hydrophilic, bioinert, nonfouling surface and a high surface density of immobilized proteins. In the present work, we developed and optimized a technique to produce a cost-effective CMD-based patterned surface for the immobilization of biomolecules to be used on standard protocols optimization. They consist of silicon or glass substrates with patterned bioactive areas able to efficiently confine the sampling solution by simply exploiting hydrophilic/hydrophobic patterning of the surface. The fabrication process involves the use of low-cost instruments and techniques, compatible with large scale production. The devices were validated through a chemiluminescence assay we recently developed for the analysis of binding of DNA nanoassemblies modified with an affinity binder to target proteins immobilized on the bioactive areas. Through this assay we were able to characterize the chemical reactivity of two target proteins toward a dextran matrix on patterned surfaces and to compare it with model CMD-based surface plasmon resonance (SPR) surfaces. We found a high reproducibility and selectivity in molecular recognition, consistent with results obtained on SPR sensor surfaces. The suggested approach is straightforward, cheap, and provides the means to assess patterned functionalized surfaces for bioanalytical platforms.

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Section: ■ Results and Discussionmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 91%

Patterned Carboxymethyl-Dextran Functionalized Surfaces Using Organic Mixed Monolayers for Biosensing Applications

Ambrosetti

Conti

Teixeira

et al. 2022

ACS Appl. Bio Mater.

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“…In this regard, hydroxyl based surface tethers have been well studied. However, nitrogen rich surfaces may need the creation of an oxidation layer or silanol groups through oxygen plasma treatment or an alternative tether layer such as positive synthetic amino acid chain like Poly-L-Lysin (PLL) with amine or epoxide based functional groups on the 5' end of the aptamer such that it may react with the amino acid [28], [29].…”

Section: Discussionmentioning

confidence: 99%

Ultra-Low Power Schottky Barrier TFT-Based Neurotransmitter Detection and Regenerative Studies

et al. 2022

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There is limited work on understanding Schottky barrier (SB) field-effect transistor (FET) behavior, and subsequent evolution of sensitivity metrics under wet detection conditions for neurotransmitter sensing applications. In this work, we report low-power deep subthreshold (DST) characteristics of SB InGaZnO4 (IGZO) thin film transistors (TFTs) and implement this regime for sensitive and selective ex vivo detection of Dopamine against Adenosine Triphosphate (ATP). Device regeneration was performed under liquid solution environment over 34 days and sensor response was observed to drift less than two orders of magnitude. Nevertheless, the quantified trends due to buffer solution baseline versus target conjugation were virtually preserved across the TFTs. Widely improved detection metrics were extracted from the detection tests, namely the limit of detection (LoD) in the 100fM-100pM range, a binding affinity (kD) between 2.03×10 -11 -3.02×10 -9 M limits, and high sensitivity around 31-42mV/decade of target concentration. Although a steady drift with clear, repeatable differences between the device state at the start and end of the 34-day period were obtained, these devices were functional even after the regeneration test period. Such results can be used to estimate the regenerative lifetime of TFT biosensors under aptamer-immobilized solution environment detection conditions.

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“…Novel silicon nitride-based nano-photonic waveguides find increasingly application in Raman spectroscopy to enhance signal strength [90] and to miniaturize chips for SERS (surface-enhanced Raman spectroscopy) [91]. Chemical activation of silicon nitride surfaces with either aqueous or organic solutions of 3-aminopropyl triethoxysilane (APTES) led to the development of an optical biosensor allowing for immobilization of biomolecules such as proteins by covalent bonding [92]. Protein immobilization was followed by incubation with murine γ-globulin and reaction with fluorescently labelled goat antimouse IgG antibody.…”

Section: Medical Diagnosticsmentioning

confidence: 99%

Silicon Nitride, a Close to Ideal Ceramic Material for Medical Application

Heimann¹

2021

Preprint

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This topical review describes the results of recent research into and development of silicon nitride, a ceramic material with unique properties. The outcome of this ongoing research strongly encourages the use of monolithic silicon nitride and coatings as contemporary and future biomaterial for a variety of medical applications. Crystallographic structure, synthesis and processing of monolithic structures and coatings, and examples of their medical applications are covered that relate to spinal, orthopedic and dental implants, bone grafts and scaffolds, platforms for intelligent synthetic neural circuits, antibacterial and antiviral particles and coatings, optical biosensors, and nano-photonic waveguides for sophisticated medical diagnostic devices. The examples provided show convincingly that silicon nitride is destined to become a leader to replace titanium and other entrenched biomaterials in many fields of medicine.

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Functionalization of silicon dioxide and silicon nitride surfaces with aminosilanes for optical biosensing applications

Cited by 20 publications

References 32 publications

Patterned Carboxymethyl-Dextran Functionalized Surfaces Using Organic Mixed Monolayers for Biosensing Applications

Patterned Carboxymethyl-Dextran Functionalized Surfaces Using Organic Mixed Monolayers for Biosensing Applications

Ultra-Low Power Schottky Barrier TFT-Based Neurotransmitter Detection and Regenerative Studies

Silicon Nitride, a Close to Ideal Ceramic Material for Medical Application

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