Functionalization of polycarbonate with proteins; open-tubular enzymatic microreactors

Ogończyk, Dominika; Jankowski, Paweł; Garstecki, Piotr

doi:10.1039/c2lc40204a

Cited by 19 publications

(22 citation statements)

References 26 publications

(30 reference statements)

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“…Polyethyleneimine (PEI), a functional amine group containing macromolecular ligand, has been utilized for the immobilization of different enzymes and also for invertase with different supports in the literature. Yamasaki et al .…”

Section: Introductionmentioning

confidence: 99%

“…[17] Commercial significance makes invertase an ideal model for immobilization studies; consequently, there are many invertase immobilization studies with different carriers in the literature. [18][19][20] Polyethyleneimine (PEI), a functional amine group containing macromolecular ligand, has been utilized for the immobilization of different enzymes [4,[21][22][23] and also for invertase [24][25][26][27][28] with different supports in the literature. Yamasaki et al figured out a method for the attachment of invertase on cotton flannel covered with PEI.…”

Section: Introductionmentioning

confidence: 99%

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Clinoptilolite particles as a carrier for biocatalysts immobilization: invertase immobilization and characterization

Bahar

2013

Asia-Pacific J Chem Eng

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Clinoptilolite, a natural zeolite, was proposed as a carrier for immobilized biocatalyst systems by polyethyleneimine and glutaraldehyde activation. Invertase (EC 3.2.1.26, beta-fructofuranosidase) as a model biocatalyst was covalently attached onto the particles by direct chemical reaction between aldehyde groups on the particles and amine groups of the enzyme. The effects of polyethyleneimine concentration, activation agent (glutaraldehyde) concentration, pH and invertase concentration on immobilization process were determined. Approximately 7 mg invertase was immobilized on a gram of clinoptilolite particles with 78% retained activity under optimum immobilization conditions. Immobilization did not affect optimum pH of the enzyme, but it brought out a wider pH-activity curve that was accepted as the indication of better pH stability. Immobilization resulted in an increase at the optimum temperature from 55 to 60 C. The K M value of immobilized invertase biosystem was found as approximately 1.5 times higher than that of the free enzyme. Immobilization provided improvement on the thermal stability of invertase, and activity half-life of the immobilized invertase was found as approximately four times longer than that of the free invertase.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Clinoptilolite particles as a carrier for biocatalysts immobilization: invertase immobilization and characterization

Bahar

2013

Asia-Pacific J Chem Eng

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“…32 Physico-chemical immobilization on a layer of polyethyleneimine was found to be best suited and reproducible. In this way, alkaline phosphatase was entrapped into a tubular reactor.…”

Section: Enzyme Immobilization -Technique Optimizationmentioning

confidence: 99%

Biotechnical Micro-Flow Processing at the EDGE – Lessons to be learnt for a Young Discipline

Hessel

Tibhe

Noël

et al. 2014

Chem.Biochem.Eng.Q.

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EDGE denotes "Explain, Demonstrate, Guide, Enable" and comes from a concept for youth leadership development training. Biotechnical and enzymatic micro-flow reactors are a young discipline. Here the enthusiasm level mirrors its technological growth and vice versa. Rather than being a linear function, enthusiasm first goes down after sudden realization of all bottlenecks and technological shortcomings which are to overcome in a Hercules task action. However, with increasing provision of technology and security in its performance, the enthusiasm level rises again. This "valley of death" needs to be crossed to bridge to a market. The enzymatic micro-flow reactors mirror the above depicted development of their counterparts -the chemical microreactors -which is already about 20 years old. This "Déjà vu" forms a feature story which encompasses a review about enzymatic microreactors and their synthetic applications which are shown in all their facets. This compilation is structured in chapters about reactor and enzyme support technology, transport intensification, chemical intensification, process-design intensification, and finally first steps into the bio-based economy.

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“…1.0E-04 0,000E+00 025E-08 050E-08 075E-08 100E-08 125E-08 150E-08 log h (mol/m².s) Finally, from the transport coefficient value (h = 4.3 × 10 −5 m/s) for the non-fouled polymer, the diffusion coefficient of molecules through the ppTMDSO film (thickness L ppTMDSO = 1µm) was calculated using Relation (6):…”

Section: Diffusion Inside the Microsystemmentioning

confidence: 99%

“…The biological function confers new possibilities of applications particularly in biochemistry, medical analysis or for clinical diagnosis [4,5]. Most of the time, the biofunctionalization was performed after microfabrication by exploiting the interaction properties of the support with biomolecules or the binding properties of linkage agents [6]. However, these kinds of immobilization processes often require numbers of preparation steps, a significant consumption of…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Effect of Plasma Polymerized Siloxane Coating for Enzyme Immobilization and Microfluidic Device Conception

et al. 2016

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This paper describes the impact of a physical immobilization methodology, using plasma polymerized 1,1,3,3, tetramethyldisiloxane, on the catalytic performance of β-galactosidase from Aspergillus oryzae in a microfluidic device. The β-galactosidase was immobilized by a polymer coating grown by Plasma Enhanced Chemical Vapor Deposition (PEVCD). Combined with a microchannel patterned in the silicone, a microreactor was obtained with which the diffusion through the plasma polymerized layer and the hydrolysis of a synthetic substrate, the resorufin-β-D-galactopyranoside, were studied. A study of the efficiency of the immobilization procedure was investigated after several uses and kinetic parameters of immobilized β-galactosidase were calculated and compared with those of soluble enzyme. Simulation and a modelling approach were also initiated to understand phenomena that influenced enzyme behavior in the physical immobilization method. Thus, the catalytic performances of immobilized enzymes were directly influenced by immobilization conditions and particularly by the diffusion behavior and availability of substrate molecules in the enzyme microenvironment.

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Functionalization of polycarbonate with proteins; open-tubular enzymatic microreactors

Cited by 19 publications

References 26 publications

Clinoptilolite particles as a carrier for biocatalysts immobilization: invertase immobilization and characterization

Clinoptilolite particles as a carrier for biocatalysts immobilization: invertase immobilization and characterization

Biotechnical Micro-Flow Processing at the EDGE – Lessons to be learnt for a Young Discipline

Investigation of the Effect of Plasma Polymerized Siloxane Coating for Enzyme Immobilization and Microfluidic Device Conception

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