Functionalization of micro-Hall effect sensors for biomedical applications utilizing superparamagnetic beads

High efficiency Hall effect micro-biosensor platform for detection of magnetically labeled biomolecules

“…Problems related to the insufficient (for micro-scale experiments with the sensors) surface coverage were encountered in the studies reported previously (Lapicki et al, 2005). On the other hand, studies on the sensitivity of Hall sensors clearly indicate that the opposite would be more desirable in order for high sensitivity, where the size of the beads and sensor should be more or less equal for best results (Sandhu et al, 2004).…”

“…A detailed description of the fabrication and characteristics of the Hall sensors was published elsewhere . The micro-current lines were designed for sensors with 5 m × 5 m active areas, and consisted of 100 nm thick films of Ti and Au, capped with 200 nm silicon nitride layer deposited by RF-magnetron sputtering of Si 3 N 4 (Lapicki et al, 2005). An additional Ti/Au bilayer was deposited to facilitate chemical bonding of thiolated probe oligonucleotides with the gold surface for immobilization experiments using hybridizing oligonucleotides.…”

Novel Nano-Heterostructure Materials and Related Devices

“…Recent applications of Hall effect devices include scanning Hall probe microscopy (SHPM) for magnetic imaging ferromagnetic domains [77][78][79] and more recently biosensing platforms incorporating superparamagnetic labels [80][81][82][83].…”

Monitoring DNA Hybridization by Quantification of Nitrogen Content Using X-Ray Photoelectron Spectroscopy

“…Biosensor systems with magnetic markers offer a potential for quantitative analysis and additional benefits in facilitating manipulation by means of applied magnetic fields. 2,3) However, labeling imposes additional time and cost constraints, and may interfere with the biorecognition interactions. Thus methods that do not require labeling such as optical biosensors, acoustic systems, microcalorimetry, field-effect transistors, and the electron spectroscopy are of great interest.…”