Functionalization of Imprinted Nanopores in Nanometer‐Thin Organic Materials

Dergunov, Sergey A.; Pinkhassik, Eugene

doi:10.1002/anie.200803261

Cited by 48 publications

(75 citation statements)

References 37 publications

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“…3,4,6 As we have previously shown, hydrophobic molecules, such as glucose pentaesters, can be dissolved in the bilayer interior together with monomers (Figure 8). …”

Section: Resultsmentioning

confidence: 71%

Scattering Studies of Hydrophobic Monomers in Liposomal Bilayers: An Expanding Shell Model of Monomer Distribution

et al. 2011

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Hydrophobic monomers partially phase-separate from saturated lipids when loaded into lipid bilayers in amounts exceeding 1:1 monomer:lipid molar ratio. This conclusion is based on agreement between two independent methods of examining the structure of monomer-loaded bilayers. Complete phase separation of monomers from lipids would result in increase in bilayer thickness and slight increase in the diameter of liposomes. Homogeneous distribution of monomers within the bilayer would not change the bilayer thickness and would lead to the increase in the liposome diameter. The increase in bilayer thickness, measured by the combination of small angle neutron scattering (SANS) and small angle X-ray scattering (SAXS), was approximately one half of what was predicted for complete phase separation. The increase in liposome diameter, measured by dynamic light scattering (DLS), was in the middle between values predicted for homogeneous distribution and complete phase separation. Combined SANS, SAXS, and DLS data suggest that at 1.2 monomer:lipid ratio, approximately one half of monomers are located in an interstitial layer sandwiched between lipid sheets. These results expand our understanding of using self-assembled bilayers as scaffolds for directed covalent assembly of organic nanomaterials. In particular, partial phase separation of monomers from lipids corroborates successful creation of nanometer-thin polymer materials with uniform imprinted nanopores. Pore-forming templates do not need to span the lipid bilayer to create a pore in the bilayer-templated films.

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“…3,4,6 As we have previously shown, hydrophobic molecules, such as glucose pentaesters, can be dissolved in the bilayer interior together with monomers (Figure 8). …”

Section: Resultsmentioning

confidence: 71%

Scattering Studies of Hydrophobic Monomers in Liposomal Bilayers: An Expanding Shell Model of Monomer Distribution

et al. 2011

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“…Here we used a molecule prepared by the coupling of glucose tetraacetate with 4-vinylbenzoic acid 5,13. 4-Vinylbenzoic moiety co-polymerizes with TBS and DVB.…”

Section: Resultsmentioning

confidence: 99%

“…Synthesis of glucose tetraacetate coupled with 4-vinylbenzoic acid5 and Reactive Blue dye coupled with β–cyclodextrin4 was described previously. All other chemicals and Sephadex G-50 were purchased from Sigma-Aldrich.…”

Section: Methodsmentioning

confidence: 99%

Synthesis, Characterization, and Long-Term Stability of Hollow Polymer Nanocapsules with Nanometer-Thin Walls

et al. 2010

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Hollow polymer nanocapsules are produced by the polymerization within hydrophobic interior of lipid bilayers that act as temporary self-assembled scaffolds. Pore-forming templates are codissolved with monomers in the bilayers to create pores with controlled size and chemical environment. Polymerization was monitored with UV spectroscopy and dynamic light scattering. High resolution magic angle spinning NMR characterization provided detailed structural information about nanocapsules. Spherical shape was confirmed by electron microscopy. Mediumsized molecules can be entrapped within porous nanocapsules. No release of encapsulated molecules was observed within 240 days.

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“…We have used lipid bilayers for the synthesis of rigid organic nanodisks and nanothin membranes containing nanopores with programmed size and chemical environment 1-3. Multiple groups have used liposomes previously to form polymer nanocapsules 4-11.…”

Section: Introductionmentioning

confidence: 99%

Time-Resolved Loading of Monomers into Bilayers with Different Curvature

et al. 2009

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Directed assembly of nanostructures within temporary and recyclable self-assembled scaffolds is emerging as an attractive method for the synthesis of nanomaterials with programmed properties. Understanding interactions of building blocks with amphiphilic scaffolds is critical for rational design of new nanostructures and nanodevices. Here we examine loading of hydrophobic monomers into bilayers with different curvatures. Time-resolved loading was studied by high performance liquid chromatography and dynamic light scattering. Despite differences in initial bilayer geometry, loading rates and maximum bilayer capacity are the same for liposomes with radii ranging from 25 to 100 nm. When using divinylbenzene (DVB) and dimyristoylphosphatidylcholine (DMPC), monomer/lipid loading ratio of 1.2 was achieved within 12 hours. While accommodation of a large amount of monomers is likely to be accompanied with significant changes in bilayer structure, all liposomes in this study including those with smallest size and higher bilayer curvature retain encapsulated content and show no evidence of fusion during monomer loading. These results contribute to our understanding of interactions between hydrophobic molecules and lipid bilayers and expand the scope of the directed assembly method.

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Functionalization of Imprinted Nanopores in Nanometer‐Thin Organic Materials

Cited by 48 publications

References 37 publications

Scattering Studies of Hydrophobic Monomers in Liposomal Bilayers: An Expanding Shell Model of Monomer Distribution

Scattering Studies of Hydrophobic Monomers in Liposomal Bilayers: An Expanding Shell Model of Monomer Distribution

Synthesis, Characterization, and Long-Term Stability of Hollow Polymer Nanocapsules with Nanometer-Thin Walls

Time-Resolved Loading of Monomers into Bilayers with Different Curvature

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