Functionalization of gold surfaces with copoly(DMA-NAS-MAPS) by dip coating: Surface characterization and hybridization tests

Abstract

a b s t r a c tIn this work, a new method to functionalize a gold surface by dip coating with a functional copolymer is presented. The coating procedure is simple, robust and can be accomplished in less than one hour. Atomic force microscopy (AFM) scratch tests reveal the presence of a homogeneous polymer coating with a thickness of 2.5 nm. X-ray photoemission spectroscopy spectra from C1s, N1s and O1s levels present the typical fingerprints of the polymeric overlayer, i.e. the characteristic peaks from CNC O and NC O groups.Surface plasmon resonance (SPR) binding assays were used to check the coating functional properties. Immobilization of heparin to SPR gold surfaces functionalized with copoly(DMA-NAS-MAPS)-followed by binding analysis with the well known heparin binding protein fibroblast growth factor 2 yield binding kinetic parameters comparable to those obtained with commercially available carboxymethyl dextranfunctionalized sensorchips, thus confirming the great potential of the proposed technique.

Click Chemistry Immobilization of Antibodies on Polymer Coated Gold Nanoparticles

“…15 This polymer is extremely versatile, and a wide number of functional monomers can be added to the basic structure of poly(DMA-MAPS) by random radical polymerization, in order to confer to it specific properties; examples of various monomers that have been used include active ester, oxyrane, 21 and ionizable groups. 22 The members of this polymer family adhere to a variety of different materials including glass, silicon oxide, 17 gold, 23 PDMS, and thermoplastics 24 by a combination of a chemi-and physisorption mechanism. Thanks to the MAPS monomer that promotes silanol condensation with hydroxyl groups introduced onto the surface by an oxygen plasma treatement, the weak noncovalent interactions between the AuNPs surface and the DMA segment are reinforced leading to the formation of an extremely stable nanometric layer.…”

“…Different strategies of 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 different materials including glass, silicon oxide 17 , gold 23 , PDMS and thermoplastics 24 by a combination of a chemi-and physisorption mechanism. Thanks to the MAPS monomer that promotes silanol condensation with hydroxyl groups introduced onto the surface by an oxygen plasma treatement, the weak non-covalent interactions between the AuNPs surface and the DMA segment are reinforced leading to the formation of an extremely stable nanometric layer.…”

Reactive Microcontact Printing of DNA Probes on (DMA-NAS-MAPS) Copolymer-Coated Substrates for Efficient Hybridization Platforms

“…30 The homogeneity of the copolymer coating is remarkable, as shown by atomic force microscopy (AFM) images of the glass-coated sample (Figure S2). The surface roughness (R RMS ) is 0.754 nm, to be compared with the nominal thickness of the copolymer layer (2.5 nm), 29 and there is no trace of uncovered areas.…”

Integrated platform for detecting pathogenic DNA via magnetic tunneling junction-based biosensors

“…First, the chips were coated with a functional copolymer (DMA-NAS-MAPS) [36] in order to provide active ester moieties suitable for immobilization of amino modified oligonucleotides and, at the same time, prevent non-specific adsorption of biological fluids components. Copoly(DMA-NAS-MAPS) has been extensively used as a functional coating in DNA microarrays [37][38][39][40][41] and various biosensing applications [22,42,43] for its favorable characteristics in terms of simplicity of use, high probe binding capacity and excellent anti-fouling properties. The probe oligonucleotides (20 µM, from MWG Biotech AG Germany), complementary or uncorrelated to the target DNA strands and modified with an amino group for allowing the covalent binding to the polymer, were then spotted on the different sensors surface, so as to implement positive and control bioassays on the very same chip.…”