Functionalization of AlN surface and effect of spacer density on Escherichia coli pili-antibody molecular recognition

Cao, Ting; Wang, Anfeng; Liang, Xuemei; Tang, Haiying; Auner, Gregory W.; Salley, Steven O.; Ng, Koon‐Kwan

doi:10.1016/j.colsurfb.2007.11.023

Cited by 15 publications

(4 citation statements)

References 39 publications

(52 reference statements)

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“…6,7 The development of detection methods that are specific for targeted biomolecules and are easy to carry out is necessary for medical diagnostics, clinical analysis, or even field tests. [8][9][10] Biosensors containing organic molecules offer promising solutions due to their speed, simplicity, and continuous monitoring capability. 11 Such sensors could comprise antibodies immobilized via organic molecules on glass sur-face, e.g., of a microstructured fiber, 12 the Au surface of a semiconductor surface plasmon resonance device, 13 or a Si surface.…”

Section: Introductionmentioning

confidence: 99%

Specific immobilization of influenza A virus on GaAs (001) surface

et al. 2009

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In the quest for the development of an all-optical biosensor for rapid detection and typing of viral pathogens, we investigate biosensing architectures that take advantage of strong photoluminescence emission from III-V quantum semiconductors (QS). One of the key elements in the development of such a biosensor is the ability to attach various analytes to GaAs--a material of choice for capping III-V QS of our interest. We report on the study of biofunctionalization of GaAs (001) with polyethylene-glycol (PEG) thiols and the successful immobilization of influenza A virus. A diluted solution of biotinylated PEG thiols in OH-terminated PEG thiols is used to form a network of sites for the attachment of neutravidin. Biotinylated polyclonal influenza A antibodies are applied to investigate the process of the immobilization of inactivated influenza A virus. The successful immobilization is demonstrated using atomic force microscopy and fluorescence microscopy measurements.

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Section: Introductionmentioning

confidence: 99%

Specific immobilization of influenza A virus on GaAs (001) surface

et al. 2009

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“…There are two types of the immobilisation of the biomolecular physical adsorption and chemical immobilisation. Physical adsorption is very sensitive to environmental condition and the second method is very stable due to the creation of covalent bonds during functionalisation [1]. The most common methods used for chemical immobilisation are antibody [2][3][4][5][6][7] and polymerase chain reaction (PCR) [8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Surface functionalisation of TiO2 evanescent waveguide sensor for E.coli monitoring

Purniawan

Pandraud

Vakalopoulos³

et al. 2012

Optical Sensing and Detection II

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This work reports the surface functionalisation of evanescent waveguide sensors to immobilise E. coli. In biosensors, the surface functionalisation is an important treatment to ensure that the sensor properly detects the cells of interest. In this paper, we study the thin film surface functionalisation of a TiO 2 evanescent waveguide sensor and their effect on light transmission for the early detection of E. coli in post colon surgery. TiO 2 deposited using atomic layer deposition (ALD) is used as waveguide material. Four layers are used in the functionalisation : the self-assembled monolayer (SAM), the protein, 1-ethyl-3-(3-dimethylaminopropyl) (EDC) and the antibodies. Aminopropyltriethoxysilane (APTES) is used as SAM and reacts with -OH group (hydroxyl). The -OH group must be provided on substrate. In order to have the proper -OH group we deposited 10 nm SiO 2 on the waveguides using PECVD and then treated the samples in oxygen plasma chamber for 2 minutes to create the groups. Afterward APTES is immediately applied on the surface after every layers of the functionalisation process. The second layer (Protein A) of the functionalisation is then put on APTES as interlayer. EDC is used as crosslink agent between APTES and antibodies. The light of Superluminescent light emitting diodes (SLEDs) (λ = 1.3 μm, 400 mA) is channelled using an optical fibre into the functionalised waveguides. The transmitted light is measured with a photodiode. The sensitivity of the sensor was evaluated using several different drain fluid concentrations in medium.

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“…The design and microfabrication of functional molecular surfaces are the key steps in the development of such nitride-based microdevices. At present, organosilane self-assembled monolayers (SAMs) have been intensely used as the cross-linkers to immobilize capture or probe molecules onto group-III nitride surfaces [4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Formation of Antibody Microarrays on Aluminum Nitride Surface Using Patterned Organosilane Self-Assembled Monolayers

2009

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Surface biofunctionalization of group-III nitride semiconductors has recently attracted much interest due to their biocompatibility, nontoxicity, and long-term chemical stability under demanding physiochemical conditions for chemical and biological sensing. Among III-nitrides, aluminum nitride (AlN) and aluminum gallium nitride (AlGaN) are particularly important because they are often used as the sensing surfaces for sensors based on field-effect transistor or surface acoustic wave sensor structures. Patterned self-assembled monolayer (SAM) templates are composed of two types of organosilane molecules terminated with different functional groups (amino and methyl), which were fabricated on AlN/sapphire substrates by combining photolithography, lift-off process, and self-assembly technique. Clear imaging contrast of SAM micropatterns can be observed by field emission scanning electron microscopy (FE-SEM) operating at a low accelerating voltage in the range of 0.5-1.5 kV. In this work, the formation of green fluorescent protein (GFP) antibody microarrays was demonstrated by the specific protein binding of enhanced GFP (EGFP) labeling. The observed strong fluorescent signal from antibody functionalized regions on the SAM-patterned AlN surface indicates the retained biological activity of specific molecular recognition resulting from the antibody-EGFP interaction. The results reported here show that micropatterning of organosilane SAMs by the combination of photolithographic process and lift-off technique is a practical approach for the fabrication of reaction regions on AlN-based bioanalytical microdevices.

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Functionalization of AlN surface and effect of spacer density on Escherichia coli pili-antibody molecular recognition

Cited by 15 publications

References 39 publications

Specific immobilization of influenza A virus on GaAs (001) surface

Specific immobilization of influenza A virus on GaAs (001) surface

Surface functionalisation of TiO2 evanescent waveguide sensor for E.coli monitoring

Formation of Antibody Microarrays on Aluminum Nitride Surface Using Patterned Organosilane Self-Assembled Monolayers

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