Functionalization, Immobilization and Stabilization of Biomolecules in Microfluidic Devices

Jha, Sandeep Kumar; Soni, Amrita; Raj, Rishi; Bala, Smriti; Sharma, Komal; Panwar, Shweta; Singh, Harpreet

doi:10.1007/978-981-15-7998-1_14

Cited by 2 publications

(2 citation statements)

References 90 publications

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“…Recognition elements can be immobilized on the electrode surface in various ways, such as physical adsorption, covalent binding, biotinavidin interaction, electropolymerization, self-assembled monolayers, etc. [33,[183][184][185][186]. The first two methods are the most common.…”

Section: Biorecognition Elements For Protein Detectionmentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

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Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics.

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Section: Biorecognition Elements For Protein Detectionmentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

View full text Add to dashboard Cite

show abstract

“…The material has been deemed suitable for multilayer device fabrication where stable silicon bonding can be formed at low temperatures. [85] However, with the development of new materials, only a few studies published since 2017 utilized surface-functionalized silicon-based microfluidic devices for heavy metal ion detection. Santangelo et al [86] developed a 3D-printed disposable silicon microfluidic device for lead Pb 2+ ion detection in the liquid phase.…”

Section: Substrate Type: Siliconmentioning

confidence: 99%

Microfluidics Evolution and Surface Functionalization: A Pathway to Enhanced Heavy Metal Ion Detection

Xu,

Jaiswal,

Liu

et al. 2024

Advanced Sensor Research

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This review delves into the significant advancements in microfluidic technology since 2017, highlighting its critical role in shrinking device sizes and integrating advanced surface functionalization techniques. It showcases how microfluidics, an interdisciplinary field, has revolutionized fluid manipulation on a microscale, enabling the creation of cost‐effective, portable devices for on‐the‐spot analyses, like heavy metal ion detection. From its early days rooted in ancient observations to cutting‐edge uses of materials like silicon, glass, polydimethylsiloxane (PDMS), and paper, this review charts microfluidics’ dynamic evolution. It emphasizes the transformative impact of surface functionalization methods, including silanization and plasma treatments, in enhancing device materials' performance. Moreover, this review anticipates the exciting convergence of microfluidics with emerging technologies like droplet microfluidics and three‐dimensional (3D) printing, alongside nanotechnology, forecasting a future of sophisticated analytical tools, point‐of‐care diagnostics, and improved detection systems. It acknowledges the hurdles in scaling production and achieving universal reliability and standardization. This review highlights the transformative impact of microfluidic technology on diagnostics and environmental surveillance, emphasizing its utility in deploying compact sensors for comprehensive and concurrent evaluations of water quality.

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Functionalization, Immobilization and Stabilization of Biomolecules in Microfluidic Devices

Cited by 2 publications

References 90 publications

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Microfluidics Evolution and Surface Functionalization: A Pathway to Enhanced Heavy Metal Ion Detection

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