Functionality and DNA-damage properties of blood cells in lactating cows exposed to glyphosate contaminated feed at different feed energy levels

Schnabel, Karina; Schmitz, Rolf; Frahm, Jana; Meyer, Ulrich; Breves, Gerhard; Dänicke, Sven

doi:10.1080/1745039x.2020.1718474

Cited by 15 publications

(27 citation statements)

References 40 publications

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“…In order to address these questions and investigate the interactions between GLY and CFP, a feeding trial was conducted comprising four feeding groups (14-16 animals/group) arranged in complete two by two factorial design. First results of this study showed no adverse effects of the tested GLY formulation on performance, energy metabolism, health characteristics and hematological parameters [18,19]. In order to get further insights, putative hepatotoxicity of GLY at different hepatic nutrient status was addressed in the present investigations.…”

Section: Introductionmentioning

confidence: 83%

“…A detailed description of the experiments and procedures for this study was published together with results concerning the effects of GLY residues and different CFP on performance, energy metabolism, health characteristics as well as hematological parameters and oxidative stress [18,19]. In short, 61 lactating German Holstein cows (207 ± 49 d in milk; parity of 2.8 ± 1.9 (S1 Table ), mean ± standard deviation) received a total mixed ration (TMR) consisting of 30% maize silage, 30% grass silage, 40% concentrate on dry matter (DM) basis for one week (week 0) as an adaption period.…”

Section: Animal Trialmentioning

confidence: 99%

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Effects of glyphosate residues and different concentrate feed proportions in dairy cow rations on hepatic gene expression, liver histology and biochemical blood parameters

et al. 2021

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Glyphosate (GLY) is worldwide one of the most used active substances in non-selective herbicides. Although livestock might be orally exposed via GLY-contaminated feedstuffs, not much is known about possible hepatotoxic effects of GLY. As hepatic xenobiotic and nutrient metabolism are interlinked, toxic effects of GLY residues might be influenced by hepatic nutrient supply. Therefore, a feeding trial with lactating dairy cows was conducted to investigate effects of GLY-contaminated feedstuffs and different concentrate feed proportions (CFP) in the diets as tool for varying nutrient supply to the liver. For this, 61 German Holstein cows (207 ± 49 days in milk; mean ± standard deviation) were either fed a GLY-contaminated total mixed ration (TMR, GLY groups, mean GLY intake 122.7 μg/kg body weight/day) or control TMR (CON groups, mean GLY intake 1.2 μg/kg body weight/day) for 16 weeks. Additionally, both groups were further split into subgroups fed a lower (LC, 30% on dry matter basis) or higher (HC, 60% on dry matter basis) CFP resulting in groups CONHC (n = 16), CONLC (n = 16), GLYHC (n = 15), GLYLC (n = 14). Blood parameters aspartate aminotransferase, γ-glutamyltransferase, glutamate dehydrogenase, cholesterol, triglyceride, total protein, calcium, phosphorus, acetic acid and urea and histopathological evaluation were not influenced by GLY, whereas all mentioned parameters were at least affected by time, CFP or an interactive manner between time and CFP. Total bilirubin blood concentration was significantly influenced by an interaction between GLY and CFP with temporarily elevated concentrations in GLYHC, whereas the biological relevance remained unclear. Gene expression analysis indicated 167 CFP-responsive genes, while seven genes showed altered expression in GLY groups compared to CON groups. Since expression changes of GLY-responsive genes were low and liver-related blood parameters changed either not at all or only slightly, the tested GLY formulation was considered to have no toxic effects on the liver of dairy cows.

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Section: Introductionmentioning

confidence: 83%

Section: Animal Trialmentioning

confidence: 99%

Effects of glyphosate residues and different concentrate feed proportions in dairy cow rations on hepatic gene expression, liver histology and biochemical blood parameters

et al. 2021

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“…In a feeding study with dairy cows, where glyphosatecontaminated feedstuffs (122.7 μg GL/kg BW for 16 weeks) were compared with control feed, no direct effects of glyphosate were detected on various blood and liver parameters (Heymann et al, 2021;Schnabel et al, 2020), as well as the general health condition of the cows (Schnabel et al, 2017). Nevertheless, long-term indirect health effects via changes in the microbiome have been documented (Gerlach et al, 2014;Krüger et al, 2013).…”

Section: Indirect Effects Of Glyphosate On Animal and Human Healthmentioning

confidence: 99%

Indirect Effects of the Herbicide Glyphosate on Plant, Animal and Human Health Through its Effects on Microbial Communities

Bruggen

Finckh

et al. 2021

Front. Environ. Sci.

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The herbicide glyphosate interferes with the shikimate pathway in plants and in major groups of microorganisms impeding the production of aromatic amino acids. Glyphosate application on plants results in a slow death, accelerated by reduced resistance to root pathogens. Extensive glyphosate use has resulted in increasing residues in soil and waterways. Although direct glyphosate effects on animals are limited, major concerns have arisen about indirect harmful side effects. In this paper, we focus on indirect effects of sublethal concentrations of glyphosate on plant, animal and human health due to shifts in microbial community compositions in successive habitats. Research results of glyphosate effects on microbial communities in soil, rhizosphere and animal guts have been contradictory due to the different integration levels studied. Most glyphosate studies have tested short-term treatment effects on microbial biomass or general community composition at higher taxonomic levels in soil, rhizosphere or animal intestinal tracts, and found little effect. More detailed studies showed reductions in specific genera or species as well as biological processes after glyphosate application. Plant growth promoting rhizobacteria and beneficial intestinal bacteria often are negatively affected, while pathogenic bacteria and fungi are enhanced. Such shifts in microbial community composition have been implicated in enhanced susceptibility of plants to Fusarium and Rhizoctonia, of birds and mammals to toxic Clostridium and Salmonella species, and of bees to Serratia and Deformed Wing Virus. In animals and humans, glyphosate exposure and concentrations in urine have been associated with intestinal diseases and neurological as well as endocrine problems, but cause-effect relationships need to be determined in more detail. Nevertheless, outbreaks of several animal and plant diseases have been related to glyphosate accumulation in the environment. Long-term glyphosate effects have been underreported, and new standards will be needed for residues in plant and animal products and the environment.

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“…Typhimurium in the RUSITEC system (Bote et al, 2019). Schnabel et al (2017) did not observe adverse effects of a glyphosate-contaminated diet (intake up to 84.5 mg/d) on performance or energy balance of dairy cows, neither did this affect leucocytes or erythrocytes (Schnabel et al, 2020) or the rumen microbiome (Billenkamp et al, 2021).…”

Section: Introductionmentioning

confidence: 92%

Effects of Different Formulations of Glyphosate on Rumen Microbial Metabolism and Bacterial Community Composition in the Rumen Simulation Technique System

et al. 2022

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The use of the herbicide glyphosate and its formulations on protein-rich feedstuff for cattle leads to a considerable intake of glyphosate into the rumen of the animals, where glyphosate may potentially impair the 5-enolpyruvylshikimate-3-phosphate pathway of the commensal microbiota, which could cause dysbiosis or proliferation of pathogenic microorganisms. Here, we evaluated the effects of pure glyphosate and the formulations Durano TF and Roundup® LB plus in different concentrations on the fermentation pattern, community composition and metabolic activity of the rumen microbiota using the Rumen Simulation Technique (RUSITEC). Application of the compounds in three concentrations (0.1 mg/l, 1.0 mg/l or 10 mg/l, n = 4 each) for 9 days did not affect fermentation parameters such as pH, redox potential, NH3-N concentration and production of short-chain fatty acids compared to a control group. Microbial protein synthesis and the degradation of different feed fractions did not vary among the treatments. None of the used compounds or concentrations did affect the microbial diversity or abundance of microbial taxa. Metaproteomics revealed that the present metabolic pathways including the shikimate pathway were not affected by addition of glyphosate, Durano TF or Roundup® LB plus. In conclusion, neither pure glyphosate, nor its formulations Durano TF and Roundup® LB plus did affect the bacterial communities of the rumen.

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Functionality and DNA-damage properties of blood cells in lactating cows exposed to glyphosate contaminated feed at different feed energy levels

Cited by 15 publications

References 40 publications

Effects of glyphosate residues and different concentrate feed proportions in dairy cow rations on hepatic gene expression, liver histology and biochemical blood parameters

Effects of glyphosate residues and different concentrate feed proportions in dairy cow rations on hepatic gene expression, liver histology and biochemical blood parameters

Indirect Effects of the Herbicide Glyphosate on Plant, Animal and Human Health Through its Effects on Microbial Communities

Effects of Different Formulations of Glyphosate on Rumen Microbial Metabolism and Bacterial Community Composition in the Rumen Simulation Technique System

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