Functionalised peptide hydrogel for the delivery of cardiac progenitor cells

Burgess, Kyle A.; Frati, Caterina; Meade, Kate; Gao, Jie; Díaz, L.; Madeddu, Denise; Graiani, Gallia; Cavalli, Stefano; Miller, Aline F.; Oceandy, Delvac; Quaini, Federico; Saiani, Alberto

doi:10.1016/j.msec.2020.111539

Cited by 33 publications

(24 citation statements)

References 45 publications

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“…Compared with collagen and Matrigel, FEK‐SAPH can self‐assemble into β‐sheet nanofibers at pH 7.2, and has good mechanical strength to improve performance (Figure 2C). 48 …”

Section: Discussionmentioning

confidence: 99%

FEK self‐assembled peptide hydrogels facilitate primary hepatocytes culture and pharmacokinetics screening

Qiao

et al. 2022

J Biomed Mater Res

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A FEFEFKFK (FEK, F, phenylalaninyl; E, glutamyl; K, lysinyl)‐based self‐assembling peptide hydrogel (FEK‐SAPH) was developed to replace sandwich culture (SC) for improved culture of primary hepatocytes in vitro. Under neutral conditions, FEK self‐assembles to form β‐sheet nanofibers, which in turn form FEK‐SAPH. For the culture of rat primary hepatocytes (RPH), the use of FEK‐SAPH simplified operation steps and promoted excellent cell–cell interactions while maintaining the SC‐related RPH polarity trend. Compared with SC, FEK‐SAPH cultured RPH for 14 days, the bile duct network was formed, the secretion of albumin and urea was improved, and the metabolic clearance rate based on cytochrome P450 (CYPs) was comparable. In FEK‐SAPH culture, the expression level of the biliary efflux transporter bile salt export pump increased by 230.7%, while the biliary excretion index value of deuterium‐labeled sodium taurocholate (d8‐TCA) differed slightly from the SC value (72% and 77%, respectively, p = .0195). The inhibitory effect of cholestasis drugs on FEK‐SAPH was significantly higher than that of SC. In FEK‐SAPH, hepatoprotective drugs were more effective in antagonizing hepatotoxicity induced by lithocholic acid (LCA). FEK‐SAPH cultured RPH with hepatoprotective drugs can better recover from LCA‐induced damage. In summary, FEK‐SAPH can be used as a substitute for SC for pharmacokinetic screening to evaluate the drug absorption, disposition, metabolism, excretion, and toxicity (ADMET) in hepatocytes.

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“…Compared with collagen and Matrigel, FEK‐SAPH can self‐assemble into β‐sheet nanofibers at pH 7.2, and has good mechanical strength to improve performance (Figure 2C). 48 …”

Section: Discussionmentioning

confidence: 99%

FEK self‐assembled peptide hydrogels facilitate primary hepatocytes culture and pharmacokinetics screening

Qiao

et al. 2022

J Biomed Mater Res

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“…CPCs (cardiac progenitor cells) have an IGF-1-IGF-1 receptor system, which can promote their survival and growth [ 123 ]. Combined intracardial injection of CPCs and this self-organized polypeptide nanofiber hydrogel showed that the volume of regenerated cardiomyocytes increased and the infarct size decreased [ 124 , 125 ]. HE-cad-Fc (Human E-cadherin fusion protein) can effectively promote the paracrine activity of MSCs (esenchymal stem cells).…”

Section: Hydrogel As a Carrier For Transplanted Cellsmentioning

confidence: 99%

Research Advances of Injectable Functional Hydrogel Materials in the Treatment of Myocardial Infarction

Yang

Guo

et al. 2022

Gels

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Myocardial infarction (MI) has become one of the serious diseases threatening human life and health. However, traditional treatment methods for MI have some limitations, such as irreversible myocardial necrosis and cardiac dysfunction. Fortunately, recent endeavors have shown that hydrogel materials can effectively prevent negative remodeling of the heart and improve the heart function and long-term prognosis of patients with MI due to their good biocompatibility, mechanical properties, and electrical conductivity. Therefore, this review aims to summarize the research progress of injectable hydrogel in the treatment of MI in recent years and to introduce the rational design of injectable hydrogels in myocardial repair. Finally, the potential challenges and perspectives of injectable hydrogel in this field will be discussed, in order to provide theoretical guidance for the development of new and effective treatment strategies for MI.

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“…The results of the study demonstrated that CPC cultured in vitro within the hydrogel spontaneously differentiated towards adult cardiac for one-week phenotypes. Furthermore, after injection, the hydrogel on its own resulted in a reduction in heart damage, and when loaded with CPC, was able to retain them for up to 10 days at the injury site, with a significant reduction in left ventricular dilation [ 122 ]. The RGD peptide was immobilized by aminolysis on a scaffold based on poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)], a bacterial copolymer with desirable mechanical and physical properties, demonstrating that the surface modification enhanced the hydrophilicity of the system, promoted the cell–scaffold interaction, and enhanced the attachment and proliferation of H9C2 myoblast cells [ 123 ] ( Figure 3 ).…”

Section: Synthetic Polymers In Cardiac Regenerationmentioning

confidence: 99%

Polymeric Biomaterials for the Treatment of Cardiac Post-Infarction Injuries

et al. 2021

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Cardiac regeneration aims to reconstruct the heart contractile mass, preventing the organ from a progressive functional deterioration, by delivering pro-regenerative cells, drugs, or growth factors to the site of injury. In recent years, scientific research focused the attention on tissue engineering for the regeneration of cardiac infarct tissue, and biomaterials able to anatomically and physiologically adapt to the heart muscle have been proposed as valuable tools for this purpose, providing the cells with the stimuli necessary to initiate a complete regenerative process. An ideal biomaterial for cardiac tissue regeneration should have a positive influence on the biomechanical, biochemical, and biological properties of tissues and cells; perfectly reflect the morphology and functionality of the native myocardium; and be mechanically stable, with a suitable thickness. Among others, engineered hydrogels, three-dimensional polymeric systems made from synthetic and natural biomaterials, have attracted much interest for cardiac post-infarction therapy. In addition, biocompatible nanosystems, and polymeric nanoparticles in particular, have been explored in preclinical studies as drug delivery and tissue engineering platforms for the treatment of cardiovascular diseases. This review focused on the most employed natural and synthetic biomaterials in cardiac regeneration, paying particular attention to the contribution of Italian research groups in this field, the fabrication techniques, and the current status of the clinical trials.

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Functionalised peptide hydrogel for the delivery of cardiac progenitor cells

Cited by 33 publications

References 45 publications

FEK self‐assembled peptide hydrogels facilitate primary hepatocytes culture and pharmacokinetics screening

FEK self‐assembled peptide hydrogels facilitate primary hepatocytes culture and pharmacokinetics screening

Research Advances of Injectable Functional Hydrogel Materials in the Treatment of Myocardial Infarction

Polymeric Biomaterials for the Treatment of Cardiac Post-Infarction Injuries

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