Functional venomics of the Sri Lankan Russell's viper (Daboia russelii) and its toxinological correlations

A proteomic analysis of Pakistan Daboia russelii russelii venom and assessment of potency of Indian polyvalent and monovalent antivenom

“…One of the LAAOs (Accession no. gi|395406796) found in Pakistan RVV was also reported in RVV from Southern India (7.9%) and Sri Lanka (5.2%) [7,8]. However, the relative abundance of LAAOs in RVV from Southern India RVV (7.9%) and Sri Lanka (5.2%) [7,8] is significantly higher as compared to Pakistan RVV (Fig.…”

“…The published reports describing mass of RVV proteins were used as references for the identification of reduced SDS-PAGE protein bands as shown in Supplementary Fig. S1 [8][9][10][11][12][13][30][31][32]. The relative abundance of RVV proteins/peptides determined by SDS-PAGE and LC-MS/MS analysis was found to be nearly identical.…”

“…The relative abundance of a protein in a particular chromatographic fraction was determined by the method described by Tan et al [8] however, with the following modifications (Eq. (1)).…”

“…By LC-MS/MS analysis of tryptic digested proteins (as well as non-tryptic peptides identified by de novo sequencing) of gel-filtration fractions, we have identified 75 proteins/peptides from NCBI databases in the Pakistan RVV proteome which belong to 14 distinct snake venom protein families. In comparison, using a proteomics approach (2D electrophoresis followed by ESI- [8].…”

“…Although venom of Russell's Vipers from different geographic origins of Indian subcontinent are well characterized [2,4,[6][7][8], with the exception of recent analyses of individual components [9][10][11][12][13], there is little information on composition, biochemical properties and pharmacological effects of RVV from Pakistan. Furthermore, snake venom is a largely untapped natural resource of novel pharmacologically active molecules [14], and only limited attempts have been made to explore the biomedically important proteins/peptides from Pakistan RVV.…”