Functional variations between Mesenchymal Stem Cells of different tissue origins: A comparative gene expression profiling

Sangeetha, K. N.; Vennila, Rosy; Secunda, R.; Sakthivel, Seethalakshmi; Pathak, Surajit; Sathyanesan, Jeswanth; Surendran, Rajagopal

doi:10.1007/s10529-020-02898-x

Cited by 12 publications

(5 citation statements)

References 50 publications

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“…By comparing gene expression profiles, Hsieh and his team found that BM-MSCs were more capable of osteogenic and adipogenic differentiation, while WJ-MSCs proliferated more [33]. In a study comparing fetal MSC sources (WJ, fetal, and the maternal side of a placenta) and adult MSCs (BM, AT), BM-MSCs showed the greatest capacity for differentiation [34]. However, not all articles go in the same direction.…”

Section: Discussionmentioning

confidence: 99%

TMT-Based Quantitative Proteomics Analysis Reveals Differentially Expressed Proteins between Different Sources of hMSCs

Naudot,

Le Ber,

Marcelo

2023

IJMS

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Mesenchymal stem cells (MSCs) are an attractive therapeutic tool for tissue engineering and regenerative medicine owing to their regenerative and trophic properties. The best-known and most widely used are bone marrow MSCs, which are currently being harvested and developed from a wide range of adult and perinatal tissues. MSCs from different sources are believed to have different secretion potentials and production, which may influence their therapeutic effects. To confirm this, we performed a quantitative proteomic analysis based on the TMT technique of MSCs from three different sources: Wharton’s jelly (WJ), dental pulp (DP), and bone marrow (BM). Our analysis focused on MSC biological properties of interest for tissue engineering. We identified a total of 611 differentially expressed human proteins. WJ-MSCs showed the greatest variation compared with the other sources. WJ produced more extracellular matrix (ECM) proteins and ECM-affiliated proteins and proteins related to the inflammatory and immune response processes. BM-MSCs expressed more proteins involved in osteogenic, adipogenic, neuronal, or muscular differentiation and proteins involved in paracrine communication. Compared to the other sources, DP-MSCs overexpressed proteins involved in the exocytosis process. The results obtained confirm the existence of differences between WJ, DP, and BM-MSCs and the need to select the MSC origin according to the therapeutic objective sought.

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Section: Discussionmentioning

confidence: 99%

TMT-Based Quantitative Proteomics Analysis Reveals Differentially Expressed Proteins between Different Sources of hMSCs

Naudot,

Le Ber,

Marcelo

2023

IJMS

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“…They exhibit a relatively high incidence of bone and cartilage formation[ 50 ], and they can generate more mature bone tissue and more compact cartilage pellets[ 118 ]. BM-MSCs express high levels of CD90, which means that they are more suitable for bone repair and regeneration[ 119 ].…”

Section: Other Common Influencing Factors In the Treatment Of Oamentioning

confidence: 99%

Factors affecting osteogenesis and chondrogenic differentiation of mesenchymal stem cells in osteoarthritis

Yi¹,

Jiang²,

Zuo³

2023

World J Stem Cells

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Osteoarthritis (OA) is a common degenerative joint disease that often involves progressive cartilage degeneration and bone destruction of subchondral bone. At present, clinical treatment is mainly for pain relief, and there are no effective methods to delay the progression of the disease. When this disease progresses to the advanced stage, the only treatment option for most patients is total knee replacement surgery, which causes patients great pain and anxiety. As a type of stem cell, mesenchymal stem cells (MSCs) have multidirectional differentiation potential. The osteogenic differentiation and chondrogenic differentiation of MSCs can play vital roles in the treatment of OA, as they can relieve pain in patients and improve joint function. The differentiation direction of MSCs is accurately controlled by a variety of signaling pathways, so there are many factors that can affect the differentiation direction of MSCs by acting on these signaling pathways. When MSCs are applied to OA treatment, the microenvironment of the joints, injected drugs, scaffold materials, source of MSCs and other factors exert specific impacts on the differentiation direction of MSCs. This review aims to summarize the mechanisms by which these factors influence MSC differentiation to produce better curative effects when MSCs are applied clinically in the future.

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“…Several studies indeed have shown that fermentation of a wide variety of foods, such as whey, citrus pomace extract, and oats, by the lactic acid bacterial group generate postbiotics (polyphenols) that cause trans-acting effects in the host’s organs [ 34 , 39 , 40 ]. Recently, it has been shown that the gut microbiome establishes a gut-skeletal muscle axis via the postbiotic metabolites (acetate and succinate) by increasing muscle energetics through mitochondrial respiration during exercise [ 41 ]. Combinative therapies with a commercially available tyndallized mixture of postbiotics ( Bifidobacterium longum and Lactobacillus acidophilus lysates) along with exercise has been shown to improve even brain function in Alzheimer’s disease mice models [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Postbiotics of Naturally Fermented Synbiotic Mixture of Rice Water Aids in Promoting Colonocyte Health

Anbalagan,

Nandabalan,

Sankar

et al. 2024

Biomolecules

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The eubiotic state of the gut microbiota is primarily brought about by various probiotic species that colonize the gut. It is becoming very clear that the probiotic-metabolite mixtures in the gut luminal milieu is central in establishing cross-kingdom signalling networks to maintain gut-multi-organ axes health. Culturally, different fermented foods and beverages have been regional staples since ancient times, and are known to be enriched with probiotics. However, regional variations including the environment, the staple food source (prebiotics), and fermentation methods, among other factors, influence the fermenting probiotic species. Fermented rice water (FRW), an economical, easy to make, simple beverage is a rich source of synbiotics. Therefore, consumption of fermented rice water allows for the intake of a variety of region-specific live probiotics. The secondary metabolites (postbiotics) present in such symbiotic mixtures may also contribute toward maintaining normal intestinal cellular functions. In this study, we highlight that regional staples such as rice consumed in their fermented form may hold promise in alleviating gut-related diseases. Our results show that simple overnight fermentation of cooked edible rice enables the growth of probiotic bacterial species belonging to the Lactic Acid Bacteria group (Leuconostoc lactis, Weisella confusa, Weisella cibacria, Lactococcus lactis, lactococcus taiwanensis, Lactobacillus fermentum, Lactobacillus nagelii, and Lactobacillus delbrueckii ssp. indicus). Metabolomic analysis of the overnight fermented and over two-nights fermented rice water identified more than 200 postbiotic metabolites. Our results show that postbiotics contributing to energy metabolism, gut-multiorgan axes, and microbial paraprobiotics are enriched in the overnight (~10 h) fermented rice water as compared to the over two-nights fermented rice water. Functional analysis via gene expression studies for nutrient absorption (mct-1 and mct-2) and barrier integrity (occludin and zo-1) reveals significant upregulation of these genes upon FRW treatment of HT29 colon cells. This study is a first-of-its-kind to demonstrate the proof-of-principle that postbiotics of naturally fermented rice water positively modulates colonocyte health.

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Functional variations between Mesenchymal Stem Cells of different tissue origins: A comparative gene expression profiling

Cited by 12 publications

References 50 publications

TMT-Based Quantitative Proteomics Analysis Reveals Differentially Expressed Proteins between Different Sources of hMSCs

TMT-Based Quantitative Proteomics Analysis Reveals Differentially Expressed Proteins between Different Sources of hMSCs

Factors affecting osteogenesis and chondrogenic differentiation of mesenchymal stem cells in osteoarthritis

Postbiotics of Naturally Fermented Synbiotic Mixture of Rice Water Aids in Promoting Colonocyte Health

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