Functional Transient Receptor Potential Vanilloid 1 is Expressed in Human Urothelial Cells

Charrua, Ana; Reguenga, Carlos; Cordeiro, Miguel; Correia‐de‐Sá, P.; Paule, C.; Nagy, I.; Cruz, Francisco; Avelino, António

doi:10.1016/j.juro.2009.08.022

Cited by 55 publications

(60 citation statements)

References 23 publications

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“…Charrua et al (11) were able to demonstrate TRPV1 currents in human cultured urothelial cells while Xu et al (75) did not see TRPV1 in freshly isolated guinea pig urothelial cells using the same technique.…”

Section: Facs or Magnetically Activated Cell Sorting (Macs)mentioning

confidence: 99%

“…Since that study, others have provided evidence to support the claim that TRPV1 is present in human urothelium. Approaches used have included immunostaining of biopsy material (39) and functional studies, including patch clamping of human urothelial cells (11). In an interesting approach designed to differentiate the influence of TRPV1 on afferent neurons from other cell types, experiments were performed in spinal cord-injured (SCI) rats.…”

Section: Trpv1mentioning

confidence: 99%

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Defining protein expression in the urothelium: a problem of more than transitional interest

Hill

2011

American Journal of Physiology-Renal Physiology

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Yu W, Hill WG. Defining protein expression in the urothelium: a problem of more than transitional interest. Am J Physiol Renal Physiol 301: F932-F942, 2011. First published August 31, 2011 doi:10.1152/ajprenal.00334.2011.-The transitional epithelium of the bladder, the urothelium, is a challenging tissue to study due to its fragility, complex cellular makeup, stratified composition, and intimate connections to both neural and connective tissue elements. With the increasing focus on the urothelium as a mechanosensory tissue with complex autocrine and paracrine signaling activities, there have arisen a number of unresolved controversies in the urothelial literature regarding whether certain important sensory and signaling proteins are expressed by the urothelium. Prominent examples of this include the transient receptor potential (TRP) family member TRPV1 and the purinergic receptor P2X 3. The problem is more than one of scientific bookkeeping since studies utilizing genetic models (primarily knockout mice) claim additional credibility for urothelial functions when phenotypes are discovered. Furthermore, both of the above-mentioned receptors are important therapeutic targets for various bladder disorders including inflammatory and neuropathic pain. The reasons for the confusion about urothelial expression are manifold, but they likely include low expression levels in some cases, poor specificity of antibodies (sometimes lacking adequate controls), the presence of nonurothelial cells resident within the urothelium, and the fact that the urothelium is particularly prone to aspecific adsorption of antibodies. In this review, we attempt to summarize some of the pitfalls with currently accepted practices in this regard, as well as to describe a set of guidelines which will improve the reliability of conclusions related to urothelial expression. It is hoped that this will be of value to investigators studying the urothelium, to those attempting to interpret conflicts in the literature, and hopefully also those charged with reviewing unpublished work. These recommendations will outline a set of "baseline" and "best practice" guidelines by which both researchers and reviewers will be able to evaluate the evidence presented.

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Section: Facs or Magnetically Activated Cell Sorting (Macs)mentioning

confidence: 99%

Section: Trpv1mentioning

confidence: 99%

Defining protein expression in the urothelium: a problem of more than transitional interest

Hill

2011

American Journal of Physiology-Renal Physiology

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“…Capsaicin itself is not selective for TRPV1 either (see Szallasi and Blumberg, 1999). There is also a discrepancy between functional data: in particular, two independent groups were unable to replicate the capsaicin effects detected in human urothelial cells (Charrua et al, 2009b) using mouse (Yamada et al, 2009) and guinea pig (Xu et al, 2009b) urothelial cells, respectively (which may represent another striking species-related difference in TRP expression). Parenthetically, adding to the confusion, increased TRPV1 expression and capsaicin-evoked ATP release was recently reported in cultured human urothelial cells obtained from bladder biopsies of overactive bladder patients compared with control subjects .…”

Section: Transient Receptor Potential Channels As Therapeutic Targmentioning

confidence: 99%

Transient Receptor Potential Channels as Drug Targets: From the Science of Basic Research to the Art of Medicine

2014

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“…TRPV1 is present and active both in the urothelium and in the nerve fibers of several species including humans (69). TRPV4 was initially described in the urothelium of rodents and humans (146).…”

Section: Trp Channel Antagonistsmentioning

confidence: 99%

Urothelial Signaling

Birder

Andersson

2013

Physiological Reviews

381

362

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The urothelium, which lines the inner surface of the renal pelvis, the ureters, and the urinary bladder, not only forms a high-resistance barrier to ion, solute and water flux, and pathogens, but also functions as an integral part of a sensory web which receives, amplifies, and transmits information about its external milieu. Urothelial cells have the ability to sense changes in their extracellular environment, and respond to chemical, mechanical and thermal stimuli by releasing various factors such as ATP, nitric oxide, and acetylcholine. They express a variety of receptors and ion channels, including P2X3 purinergic receptors, nicotinic and muscarinic receptors, and TRP channels, which all have been implicated in urothelial-neuronal interactions, and involved in signals that via components in the underlying lamina propria, such as interstitial cells, can be amplified and conveyed to nerves, detrusor muscle cells, and ultimately the central nervous system. The specialized anatomy of the urothelium and underlying structures, and the possible communication mechanisms from urothelial cells to various cell types within the bladder wall are described. Changes in the urothelium/ lamina propria ("mucosa") produced by different bladder disorders are discussed, as well as the mucosa as a target for therapeutic interventions.

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Functional Transient Receptor Potential Vanilloid 1 is Expressed in Human Urothelial Cells

Cited by 55 publications

References 23 publications

Defining protein expression in the urothelium: a problem of more than transitional interest

Defining protein expression in the urothelium: a problem of more than transitional interest

Transient Receptor Potential Channels as Drug Targets: From the Science of Basic Research to the Art of Medicine

Urothelial Signaling

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