Functional Transcranial Doppler Ultrasound for Monitoring Cerebral Blood Flow

Hage, Benjamin; Truemper, Edward J.; Bashford, Gregory R.

doi:10.3791/62048

Cited by 5 publications

(1 citation statement)

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“…Studies have found that Point‐of‐Care Ultrasound (POCUS) as an emergency medical clinical assessment is an important method for critically ill and injured patients. 2 , 3 , 4 Increasing evidence have proven that ultrasound technology can monitor cerebral arterial blood flow velocity in a rat model of cerebral hemorrhage 5 , 6 and is of great value for early intervention before irreversible ischemic neurological deficits. 7 Moreover, a previous study has shown that transcranial Doppler (TCD) can detect subarachnoid hemorrhage (SAH) 8 and the influence of ultrasonic contrast agents in cerebral vascular diagnosis.…”

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confidence: 99%

Ultrasound‐guided puncture into newborn rat brain

Pao

Zhang

et al. 2023

Ibrain

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Since the brain structure of neonatal rats was not fully formed during the first 4 days, it cannot be detected using ultrasound. The objective of this study was to investigate the use of ultrasound to detect changes in the normal coronal brain structure and determine the puncture depth of the location of the cortex, hippocampus, lateral ventricle, and striatum of newborn Sprague–Dawley (SD) rats of 5−15 days. The animal was placed in a prone position. The specific positions of the cortex, hippocampus, lateral ventricle, and striatum were measured under ultrasound. Then, the SD rats were punctured with a stereotaxic instrument, and dye was injected. Finally, the brains of SD rats were taken to make frozen sections to observe the puncture results. By ultrasound, the image of the cortex, hippocampus, lateral ventricle, and striatum of the rat can be obtained and the puncture depth of the cortex (8 days: 1.02 ± 0.12, 10 days: 1.02 ± 0.08, 13 days: 1.43 ± 0.05), hippocampus (8 days: 2.63 ± 0.07, 10 days: 2.77 ± 0.14, 13 days: 2.82 ± 0.09), lateral ventricle (8 days: 2.08 ± 0.04, 10 days: 2.26 ± 0.03, 13 days: 2.40 ± 0.06), and corpus striatum (8 days: 4.57 ± 0.09, 10 days: 4.94 ± 0.31, 13 days: 5.13 ± 0.10) can be accurately measured. The rat brain structure and puncture depth changed with the age of the rats. Ultrasound technology can not only clarify the brain structure characteristics of 5−15‐d rats but also guide the puncture and injection of the rat brain structure. The results of this study laid the foundation for the future use of ultrasound in experimental animal models of neurological diseases.

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