Functional Study of Transcription Factor MYB305 in Tobacco Flower Development

Abstract

iii 2.3 Results 2.3.1 Expression of myb305 gene 2.3.2 Phylogenetic Analysis 2.3.3 Nuclear localization of MYB305 2.3.4 MYB305 is a transcription activator in yeast 2.3.5 MYB305 activates transcription via its conserved C-terminus 2.3.6 Acidic residues near the MYB305 C-terminus mediate transcriptional activation 2.3.7 MYB305 binds the nec1 and nec5 promoters in vitro 2.3.8 Wildtype, but not mutant, nec1 promoter dirve GUS expression 2.3.9 Temporal expression of myb305, nec1 and nec5 2.3.10 Ectopic expression of MYB305 causes foliar expression of nec1 and nec 5 2.3.11 RNAi knockdown of MYB305 also knocks down expression of NEC1 and NEC5 2.4 Discussion Chapter 3. Knockdown of MYB305 inhibits nectary maturation and petal expansion

An R2R3-MYB transcription factor VyMYB24, isolated from wild grape Vitis yanshanesis J. X. Chen., regulates the plant development and confers the tolerance to drought

“…. N. tabacum and AmMYB330 from Antirrhinum (Figure 1B), which could inhibit plant development in transgenic plants that displayed dwarf phenotype (Tamagnone et al, 1998;Liu, 2010). Our results further demonstrated that overexpressing VyMYB24 caused plant dwarfing, resulting in growth parameters smaller than wild plants, including plant height, leaf area, flower length, and seed weight (Figure 3).…”

“…Members of MYB from other species were involved in plant development, including organ morphogenesis, flower development, and plant growth (Cheng et al, 2009 ; Seo et al, 2009 ; Lau et al, 2015 ). Homology analysis of protein sequences showed that VyMYB24 has a high similarity to NtMYB305 from N. tabacum and AmMYB330 from Antirrhinum ( Figure 1B ), which could inhibit plant development in transgenic plants that displayed dwarf phenotype (Tamagnone et al, 1998 ; Liu, 2010 ). Our results further demonstrated that overexpressing VyMYB24 caused plant dwarfing, resulting in growth parameters smaller than wild plants, including plant height, leaf area, flower length, and seed weight ( Figure 3 ).…”