Functional study of chromatin factors uncovers strong lineage determining roles and divergent behaviours between normal and malignant haematopoiesis

Abstract

Gracias a toda mi familia, mis tíos, primos y abuelos. Gracias yaya Carmen, se que desde el cielo siempre seguirás mis pasos.Gracias tato por confiar en que puedo con cada reto, por tu alegría, compromiso y criterio. Eres fundamental en mi vida.Gracias mamá y papá. Sois un gran ejemplo de trabajo, compromiso y unión. Gracias por cuidarme y acompañarme siempre. Este trabajo es para vosotros. 11 12 13 ABBREVIATIONS 3C Chromosome conformation capture 3D Three-dimensional Act1 Actin 1 ACTB Actin beta ACTL6A/B (BAF53A/B) Actin like 6A/B AEBP2 AE binding protein 2 AML Acute myeloid leukaemia AmpR Ampicillin resistance ARID1A/B (BAF250A/B) AT-rich interaction domain 1A/B ARID2 (BAF200) AT-rich interaction domain 2 Arp4-6/8 Actin-related protein 4-6/8 ASH2L Absent, small or homeotic disc-like ASXL1-3 Additional sex combs like 1-3 ATAC-seq Assay for transposase-accessible chromatin with high-throughput sequencing ATXN7L1-3 Ataxin 7 like 1-3 AUTS2 Autism susceptibility gene 2 protein B cell Lymphocyte B Ba (BASO) Basophil BAP1 BRCA1-associated protein 1 BAZ1A/B-2A/B Bromodomain adjacent to zinc finger domain 1A/B-2A/B BCL7A-C B-cell CLL/lymphoma 7 protein family member A-C BCOR/BCORL1 BCL6 B cell lymphoma 6 corepressor/BCL6 corepressor-like 1 BDF1 Bromodomain-containing factor 1 BFP Blue fluorescent protein BM Bone marrow bp Base pair BPTF Bromodomain PHD finger transcription factor BRD7/9 Bromodomain containing protein 7/9 BSA Bovine serum albumin C57BL/6J Cas9-neg (Wild-type) CAP-D2/D3/G/G2 Chromosome associated proteins D2/D3/G/G2 CAP-H/H2 Chromosome associated protein H Kleisin proteins Cas9 CRISPR-associated protein 9 cBAF Canonical BAF CBX2-8 Chromobox protein homolog 2-8 CCNC (CDK19) Cyclin-C CDCA5 (Sororin) Cell division cycle associated 5 CDK2AP1 (DOC1) Cyclin-dependent kinase 2 CDK2 associated protein 1 CDK8 Cyclin-dependent kinase 8 CECR2 Cat eye syndrome chromosome region protein 2 CF Chromatin factor CHD3 (Mi-2a)/CHD4 (Mi-2b)/CHD5 (KIAA0444) Chromodomain helicase DNA-binding protein 3-5 CHIP-seq Chromatin immunoprecipitation followed by sequencing CHRAC1 Chromatin accessibility complex subunit 1 CIMA Centre for Applied Medical Research CKIIa1/a2/b Casein kinase II alpha 1/alpha 2/beta CLP Common lymphoid progenitor COMPASS Complex of proteins associated with the Set1 CoREST REST corepressor CPM Counts per million cPRC1 Canonical PRC1 CRISPR Clustered regularly interspaced short palindromic repeats DC Dendritic cell RESULTS .