Functional study and regional mapping of 44 hormono-regulated genes isolated 
from a porcine granulosa cell library

Muscle tenderness is an important complex trait for meat quality and thus for genetic improvement through animal breeding. However, the physiological or genetic control of tenderness development in muscle is still poorly understood. In this work, using transcriptome analysis, we found a relationship between gene expression variability and tenderness. Muscle (longissimus dorsi) samples from 30 F(2) pigs were characterized by Warner-Bratzler Shear Force (WBSF) on cooked meat as a measurement of muscle tenderness. Gene expression levels were measured using microarrays for 17 muscle samples selected to represent a range of WBSF values. Using a linear regression model, we determined that samples with WBSF values above 30 N could be effectively analysed for genes exhibiting a significant association of their expression level on shear force (false discovery rate <0.05). These genes were shown to be involved in three functional networks: cell cycle, energy metabolism and muscle development. Twenty-two genes were mapped on the pig genome and 12 were found to be located in regions previously reported to contain quantitative trait loci (QTL) affecting pig meat tenderness (chromosomes 2, 6 and 13). Some genes appear therefore as positional candidate genes for QTL.

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“…Total RNA (2 μg) from the same RNA samples used in microarray experiments was reverse‐transcribed as described by Tosser‐Klopp et al. (2001).…”

Section: Methodsmentioning

confidence: 99%

A muscle transcriptome analysis identifies positional candidate genes for a complex trait in pig

et al. 2008

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“…The integrity of each RNA sample was checked by ethidium bromide staining of the gel. Before reverse transcription, DNase I treatment of RNA was performed as described [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…The comparative RT-PCR was performed as described by Tosser-Klopp et al [ 13 ]. Briefly, 0.5 μCi of α 32 P labelled dCTP (specific activity >3000 Ci.mmole -1 , Perkin Elmer) was added to each reverse transcription to monitor the transcription.…”

Section: Methodsmentioning

confidence: 99%

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Identification of differential gene expression in in vitro FSH treated pig granulosa cells using suppression subtractive hybridization

Bonnet

Frappart

Déhais

et al. 2006

Reprod Biol Endocrinol

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FSH, which binds to specific receptors on granulosa cells in mammals, plays a key role in folliculogenesis. Its biological activity involves stimulation of intercellular communication and upregulation of steroidogenesis, but the entire spectrum of the genes regulated by FSH has yet to be fully characterized.In order to find new regulated transcripts, however rare, we have used a Suppression Subtractive Hybridization approach (SSH) on pig granulosa cells in primary culture treated or not with FSH. Two SSH libraries were generated and 76 clones were sequenced after selection by differential screening. Sixty four different sequences were identified, including 3 novel sequences. Experiments demonstrated the presence of 25 regulated transcripts.A gene ontology analysis of these 25 genes revealed (1) catalytic; (2) transport; (3) signal transducer; (4) binding; (5) anti-oxidant and (6) structural activities. These findings may deepen our understanding of FSH's effects. Particularly, they suggest that FSH is involved in the modulation of peroxidase activity and remodelling of chromatin.

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Generation and characterization of a 12,000-rad radiation hybrid panel for fine mapping in pig

Yerle¹,

Pinton²,

Delcros³

et al. 2002

Cytogenet Genome Res

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We have constructed a 12,000-rad porcine whole-genome radiation hybrid panel to complement the first generation 7,000-rad panel (IMpRH) and allow higher resolution mapping studies both in specific areas of interest and on the whole genome. We analyzed 243 hybrid clones on the basis of their marker retention frequency to produce a final panel of 90 hybrid clones with an average retention frequency of 35.4%. The resolution of this 12,000-rad panel (IMNpRH2) was compared to the resolution of the 7,000-rad panel (IMpRH) by constructing framework maps in the 2.4-Mb region of porcine chromosome 15 containing the acid meat RN gene. In this region, two-point analysis was used to estimate RH distances and demonstrates their reliability with the estimation of physical distances. This study demonstrates that the 12,000-rad panel constitutes a powerful tool for constructing high-resolution maps. Indeed, the resolution of IMNpRH2 (12–14 kb/cR_12,000) is two to three times more than that of IMpRH (35–37 kb/cR_7,000). As expected, the increase in the radiation dose allows an increase of the mapping resolution in terms of kb/cR with the same suppleness of use for mapping experiments. In addition the RH map constructed in the region investigated proved to be more homogeneous on IMNpRH2 than on IMpRH.

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Functional study and regional mapping of 44 hormono-regulated genes isolated from a porcine granulosa cell library

Cited by 4 publications

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A muscle transcriptome analysis identifies positional candidate genes for a complex trait in pig

A muscle transcriptome analysis identifies positional candidate genes for a complex trait in pig

Identification of differential gene expression in in vitro FSH treated pig granulosa cells using suppression subtractive hybridization

Generation and characterization of a 12,000-rad radiation hybrid panel for fine mapping in pig

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