Functional studies of soybean (Glycine max L.) seed LEA proteins GmPM6, GmPM11, and GmPM30 by CD and FTIR spectroscopy

Shih, Ming-Der; Hsieh, Tzung-Yang; Jian, Wei-Ting; Wu, Ming‐Tsung; Yang, Shin-Jin; Hoekstra, Folkert A.; Hsing, Yue‐Ie C.

doi:10.1016/j.plantsci.2012.07.012

Cited by 43 publications

(34 citation statements)

References 50 publications

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“…Desiccation of the protein caused an increase in α-helix content from 4% in solution to 46% in the dried state. As previously shown for other LEA proteins in solution (e.g., Tolleter et al 2007; Shih et al 2012), addition of the desolvating agent trifluoroethanol (TFE) also promoted an increase in α-helices, as did the addition of SDS. Similar results were found with AfrLEA3m, i.e., it was predominantly disordered in solution and adopted a more α-helical structure after drying.…”

Section: Structural and Functional Characterization Of Lea Proteins Fsupporting

confidence: 61%

Molecular approaches for improving desiccation tolerance: insights from the brine shrimp Artemia franciscana

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Menze

2015

Planta

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Organisms inhabiting both aquatic and terrestrial ecosystems frequently are confronted with the problem of water loss for multiple reasons – exposure to hypersalinity, evaporative water loss, and restriction of intracellular water due to freezing of extracellular fluids. Seasonal desiccation can become severe and lead to the production of tolerant propagules and entry into the state of anhydrobiosis at various stages of the life cycle. Such is the case for gastrula-stage embryos of the brine shrimp, Artemia franciscana. Physiological and biochemical responses to desiccation are central for survival and are multifaceted. This review will evaluate the impact of multiple Late Embryogenesis Abundant (LEA) proteins originating from A. franciscana, together with the non-reducing sugar trehalose, on prevention of desiccation damage at multiple levels of biological organization. Survivorship of desiccation-sensitive cells during water stress can be improved by use of the above protective agents, coupled to metabolic preconditioning and rapid cell drying. However, obtaining long-term stability of cells in the dried state at room temperature has not been accomplished and will require continued efforts on both the physicochemical and biological fronts.

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Section: Structural and Functional Characterization Of Lea Proteins Fsupporting

confidence: 61%

Molecular approaches for improving desiccation tolerance: insights from the brine shrimp Artemia franciscana

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Menze

2015

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“…However, our work shows that not only different dehydration rates led to different sucrose content but also that its increased concentration alone is not enough to establish DT and prevent membrane damage resulting in high cell death (Cruz de Carvalho et al, 2012). The elevated sucrose content may be an important part of the DT mechanisms but combined with changes in the proteome (Cruz de Carvalho et al, 2014), particularly LEA proteins, contributing to form a more stable vitrified cytoplasm during slow dehydration that can improve cells DT (Shih et al, 2012), reducing the metabolism and hazardous ROS production (Crowe et al, 1992;Smirnoff, 1992).…”

Section: Osmoregulation and High Sucrose Alone Does Not Contribute Tomentioning

confidence: 78%

Influence of dehydration rate on cell sucrose and water relations parameters in an inducible desiccation tolerant aquatic bryophyte

Carvalho

Silva

Branquinho

et al. 2015

Environmental and Experimental Botany

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“…LEA proteins are believed to protect cells from desiccation-induced damage by acting as hydration buffers or by direct protection of proteins and membranes [62], [63]. Group 3 LEA transcripts predominate the dehydration transcriptome of S. stapfianus and prevent or reduce protein aggregation alone or in combination with disaccharides during dehydration in vitro [64, 65]. The ubiquity of increased LEA protein transcript abundance in the transcriptomes of angiosperm resurrection species during dehydration and their classification as seed maturation proteins underpins the hypothesis that resurrection plants evolved their mechanism of desiccation tolerance from a reprogramming of the developmentally controlled seed desiccation tolerance (first documented by [66]; and discussed by Oliver et al 2005 [4]; and more recently by Farrant and Moore 2011 [6]).…”

Section: Discussionmentioning

confidence: 99%

Sporobolus stapfianus: Insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics

et al. 2017

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BackgroundUnderstanding the response of resurrection angiosperms to dehydration and rehydration is critical for deciphering the mechanisms of how plants cope with the rigors of water loss from their vegetative tissues. We have focused our studies on the C4 resurrection grass, Sporobolus stapfianus Gandoger, as a member of a group of important forage grasses.MethodsWe have combined non-targeted metabolomics with transcriptomics, via a NimbleGen array platform, to develop an understanding of how gene expression and metabolite profiles can be linked to generate a more detailed mechanistic appreciation of the cellular response to both desiccation and rehydration.ResultsThe rehydration transcriptome and metabolome are primarily geared towards the rapid return of photosynthesis, energy metabolism, protein turnover, and protein synthesis during the rehydration phase. However, there are some metabolites associated with ROS protection that remain elevated during rehydration, most notably the tocopherols. The analysis of the dehydration transcriptome reveals a strong concordance between transcript abundance and the associated metabolite abundance reported earlier, but only in responses that are directly related to cellular protection during dehydration: carbohydrate metabolism and redox homeostasis. The transcriptome response also provides strong support for the involvement of cellular protection processes as exemplified by the increases in the abundance of transcripts encoding late embryogenesis abundant (LEA) proteins, anti-oxidant enzymes, early light-induced proteins (ELIP) proteins, and cell-wall modification enzymes. There is little concordance between transcript and metabolite abundance for processes such as amino acid metabolism that do not appear to contribute directly to cellular protection, but are nonetheless important for the desiccation tolerant phenotype of S. stapfianus.ConclusionsThe transcriptomes of both dehydration and rehydration offer insight into the complexity of the regulation of responses to these processes that involve complex signaling pathways and associated transcription factors. ABA appears to be important in the control of gene expression in both the latter stages of the dehydration and the early stages of rehydration. These findings add to the growing body of information detailing how plants tolerate and survive the severe cellular perturbations of dehydration, desiccation, and rehydration.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-1013-7) contains supplementary material, which is available to authorized users.

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Functional studies of soybean (Glycine max L.) seed LEA proteins GmPM6, GmPM11, and GmPM30 by CD and FTIR spectroscopy

Cited by 43 publications

References 50 publications

Molecular approaches for improving desiccation tolerance: insights from the brine shrimp Artemia franciscana

Molecular approaches for improving desiccation tolerance: insights from the brine shrimp Artemia franciscana

Influence of dehydration rate on cell sucrose and water relations parameters in an inducible desiccation tolerant aquatic bryophyte

Sporobolus stapfianus: Insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics

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