Functional Studies of Complementary Sense Promoter (Replicase Promoter) from Tomato Yellow Leaf Curl Virus: Evidence for Transcription Capability in Non‐host Plants
Abstract:The complementary sense promoter C1 that regulates the transcription of replicase gene in geminiviruses was cloned from tomato yellow leaf curl virus. A transcription fusion of β‐glucuronidase gene (GUS) coding sequence to complementary sense promoter, that replaced the viral replicase coding sequence, was constructed. Protoplasts of Nicotiana tabacum were electroporated to introduce the GUS gene controlled by the C1 promoter. The level of GUS transient gene expression was enhanced by the increase of the plasm… Show more
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